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New cell lines of gastric and pancreatic cancer: distinct morphology, growth characteristics, expression of epithelial and immunoregulatory antigens

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Abstract

Two new cell lines from stomach cancers and one from a pancreatic carcinoma are presented. MZ-GC-1 was established from a hepatic metastasis of a well differentiated gastric adenocarcinoma. MZ-GC-2 was derived from ascites induced by a poorly differentiated gastric adenocarcinoma. MZ-PC-1 originated from the pleural effusion of a moderately well differentiated pancreatic ductal adenocarcinoma. MZ-GC-1 cells were adherent and partially polarized, connected tightly via desmosomes. In contrast MZ-GC-2 cells consisted of slightly adherent or floating subpopulations and displayed no desmosomes. MZ-PC-1 cells were adherent and showed polarized growth, connected by apical junctional complexes. Cell doubling times were 7 days for MZ-GC-1 and 45 h for MZ-GC-2 and MZ-PC-1 cells. MZ-GC-2 and MZ-PC-1 gave rise to nude mouse tumours, resembling the original lesions. Chromosome analysis of the cell lines revealed a high range of numerical abnormalities. Each cell line had cytokeratin patterns fitting well to typical in vivo patterns. Furthermore the cell lines expressed a panel of antigens typical for gastrointestinal epithelia. Unique for MZ-PC-1 were high amounts of secreted Ca19-9. γ-Interferon enhanced HLA-class I antigens up to twofold and induced ICAM-1 expression on each cell line. HLA-class II antigens were differentially enhanced by γ-interferon. Due to their distinct characteristics the three tumour cell lines may be useful models in the investigation of the cell biology and immunogenicity of gastrointestinal tumours.

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Authors and Affiliations

  1. I. Medizinische Klinik und Poliklinik, Johannes Gutenberg-Universität, Langenbeckstrasse 1, D-55101, Mainz, Germany

    M. Heike, K. H. Meyer zum Büschenfelde & W. G. Dippold

  2. Institut für Pathologie, Johannes Gutenberg-Universität, Mainz, Germany

    O. Röhrig & R. Moll

  3. Institut für Pathologie, Heinrich Heine-Universität, Düsseldorf, Germany

    H. E. Gabbert

  4. II. Medizinische Klinik, Krankenhaus Nordwest, Frankfurt/Main, Germany

    A. Knuth

Authors
  1. M. Heike
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  2. K. H. Meyer zum Büschenfelde
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  3. W. G. Dippold
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  4. O. Röhrig
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  5. R. Moll
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  6. H. E. Gabbert
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  7. A. Knuth
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Additional information

This work has been supported by the Deutsche Forschungsgemeinschaft, Bonn, Germany (He 1752-2-1) and by an Investigator Award from the Cancer Research Institute, New York, USA (A. Knuth).

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Heike, M., Meyer zum Büschenfelde, K.H., Dippold, W.G. et al. New cell lines of gastric and pancreatic cancer: distinct morphology, growth characteristics, expression of epithelial and immunoregulatory antigens. Vichows Archiv A Pathol Anat 426, 375–384 (1995). https://doi.org/10.1007/BF00191347

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  • DOI: https://doi.org/10.1007/BF00191347

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