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Ganglienzellen und paraganglionäre Zellen des Ganglion cervicale superius der Ratte nach Parachlorophenylalanin (pCPA)

On the effects of parachlorophenylalanin on nerve cells and paraganglionic cells in the superior cervical ganglion of the rat

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Summary

The effect of parachlorophenylalanine (pCPA) on the paraganglionic and sympathetic nerve cells of the rat superior cervical ganglion was studied. pCPA influences the sympathetic nerve cells of the cervical ganglion to minimal extent. Unspecific changes of the Golgi apparatus and of the Nissl bodies as well as an increase in the number of lysosomes occur. An extended period of treatment causes degeneration of individual ganglionic cells and nerve fibers. The paraganglionic cells in control animals are characterized by dense core vesicles of an unitary type. During pCPA treatment degranulated paraganglionic cells are to be found. The degranulation of the individual cell elements varies.

Fluorescence microscopic study after pCPA application shows a shift of the fluorescence spectrum toward green. The yellow component of the control group decreases after 6 to 12 days and disappears after 18 or 20 days. It is discussed whether the fluorescence microscopic changes are due to the inhibition of serotonine synthesis or to a decrease of the catecholamine synthesis.

Zusammenfassung

Die Wirkung von Parachlorophenylalanin (pCPA) auf die paraganglionären Zellen und sympathischen Ganglienzellen des Ganglion cervicale superius der Ratte wurde untersucht. pCPA beeinflußt die sympathischen Nervenzellen im Ganglion cervicale superius geringgradig. Es finden sich unspezifische Veränderungen des Golgi-Apparates und der Nissl-Schollen sowie eine Zunahme von Lysosomen. Bei langdauernder Behandlung degenerieren einzelne Ganglienzellen und Nervenfasern. Die paraganglionären Zellen sind bei Kontrolltieren durch “dense core vesicles” eines einheitlichen Typs charakterisiert. Im Laufe der pCPA-Behandlung finden sich degranulierte paraganglionäre Zellen. Die Degranulation der einzelnen Zellelemente ist jedoch variabel.

Bei fluorescenzmikroskopischer Untersuchung zeigt sich nach pCPA-Applikation eine Verschiebung des Fluorescenzspektrums zum grünen Bereich hin. Die bei Kontrollen deutliche Gelbkomponente nimmt nach 6–12 Tagen ab und verschwindet schließlich nach 18–20 Tagen. Es wird diskutiert, ob die fluorescenzmikroskopischen Veränderungen aufgrund einer Hemmung der Serotoninsynthese oder aufgrund einer abgeschwächten Katecholaminsynthese entstehen.

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Literatur

  • Angeletti, P. U., Levi-Montalcini, R.: Sympathetic nerve cell destruction in newborn mammals by 6-hydroxydopamine. Proc. nat. Acad. Sci. (Wash.) 65, 114–121 (1970).

    Google Scholar 

  • Angeletti, P. U., Levi-Montalcini, R., Caramia, F.: Structural and ultrastructural changes in developing sympathetic ganglia induced by Guanethidine. Brain Res. 43, 515–525 (1972)

    Google Scholar 

  • Battaglia, G.: Ricerche ultrastrutturali sul glomo carotideo del ratto. Arch. ital. Anat. Embryol. 73, 155–187 (1968)

    Google Scholar 

  • Bein, H. J.: Some pharmacological properties of guanethidine in adrenergic mechanisms p. 162–172. London: CIBA Foundation (Churchill) (1960)

    Google Scholar 

  • Bloom, F. E., Giarman, N. J.: The effects of p-chlorophenylalanine on the content and cellular distribution of 5-HT in the rat pineal gland: combined biochemical and electronmicroscopic analyses. Biochem. Pharmacol. 19, 1213–1219 (1970)

    Google Scholar 

  • Böck, P.: Die Feinstruktur der peribronchialen Mikroparaganglien beim Meerschweinchen. Z. Zellforsch. 110, 243–257 (1970)

    Google Scholar 

  • Böck, P.: Das Glomus caroticum der Maus. Ergebn. Anat. Entwickl.-Gesch. 48, 1–84 (1973)

    Google Scholar 

  • Corrodi, H., Jonsson, G.: The formaldehyde fluorescence method for the histochemical demonstration of biogenic monoamines. A review on the methodology. J. Histochem. Cytochem. 15, 65–78 (1967)

    Google Scholar 

  • Edwards, D. J., Blau, K.: The in vivo formation of p-chlorophenylethylamine in young rats injected with p-chlorophenylalanine. J. Neurochem. 19, 1829–1832 (1972)

    Google Scholar 

  • Elfvin, L. G.: The ultrastructure of the superior cervical sympathetic ganglion of the cat. I. The structure of the ganglion cell processes as studied by serial sections. J. Ultrastruct. Res. 8, 403–440 (1963)

    Google Scholar 

  • Eränkö, L., Eränkö, O.: Effect of guanethidine on nerve cells and small intensely fluorescent cells in sympathetic ganglia of newborn and adult rats. Acta pharmacol. (Kbh.) 30, 403–416 (1971)

    Google Scholar 

  • Eränkö, O., Eränkö, L.: Small, intensely fluorescent granule-containing cells in the sympathetic ganglion of the rat. Progr. Brain Res. 34, 39–52 (1971)

    Google Scholar 

  • Eränkö, O., Eränkö, L.: Histochemical and electron microscopic observations on sympathetic ganglia. Proc. III. Europ. Anat. Congr. (Manchester) 21–22 (1973)

  • Eränkö, O., Härkönen, M.: Monoamine-containing small cells in the superior cervical ganglion of the rat and an organ composed of them. Acta physiol. scand. 63, 511–512 (1965)

    Google Scholar 

  • Falck, B., Hillarp, N. A., Thieme, G., Torp, A.: Fluorescence of catecholamines and related compounds condensed with formaldehyde. J. Histochem. Cytochem. 10, 348–354 (1962)

    Google Scholar 

  • Falck, B., Owman, Ch.: A detailed methodological description of the fluorescence method for the cellular demonstration of biogenic monoamines. Acta Univ. Lund, Sect. II, 7, 4–23 (1965)

    Google Scholar 

  • Forssmann, W. G.: Studien über den Feinbau des Ganglion cervicale superius der Ratte. I. Normale Struktur. Acta anat. (Basel) 59, 105–140 (1964)

    Google Scholar 

  • Forssmann, W. G.: Präparation biologischer Gewebe für die Elektronenmikroskopie. I. Fixation. Seminar f. Elektr. Mikr. Techn. Akad. Esslingen (1969)

  • Forssmann, W. G., Brühl, B.: Zur Ultrastruktur des Glomus caroticum der Ratte. Z. Anat. Entwickl.-Gesch. (in Vorbereitung)

  • Gal, E. M., Millard, S. A.: The mechanism of inhibition of hydroxylases in vivo by p-chlorophenylalanine: the effect of cyclo-heximide. Biochem. biophys. Acta (Amst.) 227, 32–41 (1971)

    Google Scholar 

  • Gal, E. M., Rogeveen, A. E., Millard, S. A.: DL-(2–14C) p-Chlorophenylalanine as an inhibitor of tryptophan-5-hydroxylase. J. Neurochem. 17, 1221–1235 (1970)

    Google Scholar 

  • Gansler, H.: Elektronenmikroskopische Untersuchungen am Grenzstrangganglion von menschlichem Operationsmaterial. Proc. of the Stockholm Conference on El. Mikr., p. 190–191. Uppsala: Almqvist and Wiksell 1957

    Google Scholar 

  • Grube, D., Aumüller, G., Forssmann, W. G.: Untersuchungen über die EC-Zelle nach Hemmung der Tryptophan-5-Hydroxylase durch PCPA. Verh. Anat. Ges. 68, Anat. Anz., Ergh. 134 (in Druck)

  • Guroff, G.: Irreversible in vivo inhibition of rat liver phenylalanine hydroxylase by p-chlorophenylalanine. Arch. Biochem. Biophys. 134, 610–611 (1969)

    Google Scholar 

  • Ito, A., Schoenberg, S. M.: Central nervous system mechanism responsible for blood pressure elevation induced by p-chlorophenylalanine. J. Pharmacol. exp. Ther. 181, 65–74 (1972)

    Google Scholar 

  • Jensen-Holm, J., Juul, P.: The effects of guanethidine, pre- and postganglionic nerve division on the rat, superior cervical ganglion: cholinesterases and catecholamines. Acta pharmacol. (Kbh.) 28, 283–298 (1970)

    Google Scholar 

  • Jequier, E., Lovenberg, W., Sjoerdsma, A.: Tryptophan hydroxylase inhibition: the mechanism by which p-chlorophenylalanine deplets rat brain serotonine. Med. Pharmacol. 3, 274–278 (1967)

    Google Scholar 

  • Knapp, S., Mandell, A. J.: Parachlorophenylalanine—its three phase sequence of interactions with the two forms of brain tryptophan hydroxylase. Life Sci. 11, 716–771 (1972)

    Google Scholar 

  • Koe, B. K., Weissman, A.: P-chlorophenylalanine: a specific depletor of brain serotonine. J. Pharmacol. exp. Ther. 154, 499–516 (1966)

    Google Scholar 

  • Koe, B. K., Weissman, A.: The pharmacology of para-chlorophenylalanine, selective depletor of serotonine stores. Advanc. Pharmacol. 6, 29–47 (1968)

    Google Scholar 

  • Laverty, R., Phelan, E. L.: Effects of 6-hydroxydopamine on noradrenaline storage and uptake in the rat. Proc. Otago med. School 47, 18–19 (1969)

    Google Scholar 

  • Lever, J. D., Presley, R.: A survey of parenchymal and other elements in sympathetic ganglia. Proc. III. Europ. Anat. Congr. (Manchester) 24–25 (1973)

  • Luft, J. H.: Improvements in epoxy resin embedding methods. J. biophys. biochem. Cytol. 9, 409–414 (1961)

    Google Scholar 

  • Matthews, M., Raisman, R.: Two cell types in the superior cervical ganglion of the rat. J. Anat. (Lond.) 103, 397–398 (1968)

    Google Scholar 

  • Matthews, M., Raisman, R.: The ultrastructure and somatic efferent synapses of small granule-containing cells in the superior cervical ganglion. J. Anat. (Lond.) 105, 255–282 (1969)

    Google Scholar 

  • McGeer, E. G., Peters, D. A., McGeer, P. L.: Inhibition of rat brain tryptophan hydroxylase by 6-halotryptophans. Life Sci. 7, 605–615 (1968)

    Google Scholar 

  • Möllmann, H., Knoche, H., Niemeyer, H., Alfes, H., Kienecker, E. W., Decker, S.: Experimenteller Beitrag zur Kenntnis der biogenen Amine im Glomus caroticum des Kaninchens. Elektronen- und fluoreszenzmikroskopische Untersuchungen nach Reserpin und pCPA. Z. Zellforsch. 124, 238–246 (1972)

    Google Scholar 

  • Möllmann, H., Niemeyer, D. H., Alfes, H., Knoche, H.: Mikrospektrofluorometrische Untersuchungen der biogenen Amine im Glomus caroticum des Kaninchens nach Reserpin- und pCPA-Applikation. Z. Zellforsch. 126, 104–115 (1972)

    Google Scholar 

  • Peters, D. A., Filczewski, M., Mazerkiewicz-Kwilecki, I. M.: Effect of parachlorophenylalanine on catecholamine-synthesis in rat brain, heart and adrenals. Biochem. Pharmacol. 21, 2282–2284 (1972)

    Google Scholar 

  • Pick, J.: Pigment, abnormal mitochondria and laminar bodies in human sympathetic neuron. An electron microscopical study. Z. Zellforsch. 82, 118–135 (1967)

    Google Scholar 

  • Reynolds, E. S.: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 17, 208–212 (1963)

    Google Scholar 

  • Ribaupierre, F. de, Siegrist, G., Dunant, Y., Dolivo, M.: Etude de la morphologie et de la fonction probable des cellules chromaffins du ganglion sympathique cervical supérieur du rat. J. Physiol. (Paris) 58, 601 (1966)

    Google Scholar 

  • Siegrist, G., Dolivo, M., Dunant, Y., Foroglou-Kerameus, C., Ribaupierre, F. de, Rouiller, Ch.: Ultrastructure and function of the chromaffine cells in the superior cervical ganglion of the rat. J. Ultrastruct. Res. 25, 381–407 (1968)

    Google Scholar 

  • Siegrist, G., Ribaupierre, F. de, Dolivo, M., Rouiller, Ch.: Les cellules chromaffins des ganglions cervicaux supérieurs du rat. J. Microscopie 6, 791–794 (1966)

    Google Scholar 

  • Smith, A. R., Kappers, J. A., Jongkind, J. F.: Alterations in the distribution of yellow fluorescing rabbit pinealocytes produced by p-chlorophenylalanine and different conditions of illumination. J. Neurol. Transmiss. 33, 91–111 (1972)

    Google Scholar 

  • Wacker, Ph., Forssmann, W. G.: Entspeicherung und Synthesehemmung von Catecholaminen in der Nebenniere der Ratte. Verh. Anat. Ges. 65, Anat. Anz., Ergh. 128, 37–50 (1971)

    Google Scholar 

  • Wende, C. v. d., Johnson, J. C.: Interaction of serotonin with the catecholamines. II. Activation and inhibition of adrenochrome formation. Biochem. Pharmacol. 19, 2001–2007 (1970)

    Google Scholar 

  • Williams, T. H.: The question of the intraganglionic (connector) neuron of the autonomic nervous system. J. Anat. (Lond.) 101, 603–604 (1967)

    Google Scholar 

  • Williams, T. H.: Electron microscopic evidence for an autonomic interneuron. Nature (Lond.) 214, 309–310 (1967)

    Google Scholar 

  • Yamauchi, A., Lever, D., Kemp, K. W.: Catecholamine loading and depletion in the rat superior cervical ganglion. J. Anat. (Lond.) 114, 271–282 (1973)

    Google Scholar 

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Authors and Affiliations

  1. Anatomisches Institut der Universität Heidelberg, Heidelberg, Deutschland

    Ch. Heym, D. Grube & W. G. Forssmann

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  1. Ch. Heym
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  2. D. Grube
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  3. W. G. Forssmann
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Mit Unterstützung der Deutschen Forschungsgemeinschaft, Antrag Fo 77/4.

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Heym, C., Grube, D. & Forssmann, W.G. Ganglienzellen und paraganglionäre Zellen des Ganglion cervicale superius der Ratte nach Parachlorophenylalanin (pCPA). Z. Anat. Entwickl. Gesch. 143, 223–237 (1974). https://doi.org/10.1007/BF00519867

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