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In vitro studies on the self-differentiating capacities of quail adenohypophysis epithelium

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Summary

Isolated epithelial rudiments of 3–4 days quail embryo adenohypophysis were cultivated in vitro. Differentiation of glandular cells occured when culture conditions allowed the formation of explants characterized by the presence of a fibroblastic sheet and of an epithelial roof surrounding a central cavity. Differentiation did not occur when culture conditions did not allow the fibroblastic sheet to be established or resulted in the absence of a cavity. The importance of the explant structure in differentiation is also indicated by the location of glandular cells. In vitro they differentiate near the cavity derived from Rathke's pouch lumen while in situ they first appear at the periphery of the rudiment, near the basement membrane.

The results of this study show that the adenohypophyseal primordium can differentiate without any mesenchymal influence. The observed differentiation seems to be correlated with fibroblast-secreted material, and this hypo-thesis is discussed.

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References

  • Augusti-Tocco, G., Chiarugi, V.P.: Surface glycosaminoglycans as a differentiation cofactor in neuroblastoma cell cultures. Cell Differ. 5, 161–171 (1976)

    Google Scholar 

  • Banerjee, S.D., Cohn, R.H., Bernfield, M.R.: Basal lamina of embryonic salivary epithelia. Production by the epithelium and role in maintening lobular morphology. J. Cell Biol. 73, 445–463 (1977)

    Google Scholar 

  • Bernfield, M.R., Banerjee, S.D.: Acid mucopolysaccharide (glycosaminoglycan) at the epithelial-mesenchymal interface of mouse embryo salivary glands. J. Cell Biol. 52, 664–673 (1972)

    Google Scholar 

  • Betz, T.W., Jarskär, R.: Chicken pars distalis development. Cell Tissue. Res. 155, 291–320 (1974)

    Google Scholar 

  • Bluemink, J.G., Van Maurik, P., Lawson, K.A.: Intimate cell contacts at the epithelial/mesenchymal interface in embryonic mouse lung. J. Ultrastruct. Res. 55, 257–270 (1976)

    Google Scholar 

  • Chiarugi, V.P.: Cell-coat glycosaminoglycans in cellular transformation and differentiation. Exp. Cell Biol. 44, 251–259 (1976)

    Google Scholar 

  • Chiarugi, V.P., Vannuchi, S.: Surface heparan sulfate as a control element in eukariotic cells: a working model. J. Theor. Biol. 61, 459–475 (1976)

    Google Scholar 

  • Conklin, J.L.: The development of the human foetal adenohypophysis. Anat. Rec. 160, 79–92 (1968)

    Google Scholar 

  • Conrad, G.W., Hart, G.W.: Heparan sulfate biosynthesis by embryonic tissues and primary fibroblast populations. Dev. Biol. 44, 253–269 (1975)

    Google Scholar 

  • Conrad, G.W., Hamilton, C., Haynes, E.: Differences in glycosaminoglycans synthesized by fibroblast-like cells from chick cornea, heart, and skin. J. Biol. Chem. 252, 6861–6870 (1977)

    Google Scholar 

  • Cutler, L.S., Chaudhry, A.P.: Intercellular contacts at the epithelial-mesenchymal interface during the prenatal development of the rat submandibular gland. Dev. Biol. 33, 229–240 (1973)

    Google Scholar 

  • Ferrand, R.: Différenciation en greffe chorio-allantoïdienne de l'epithelium de la poche de Rathke d'embryons de caille et de poulet. C.R. Soc. Biol. 163, 2669–2672 (1969)

    Google Scholar 

  • Ferrand R.: Etude expérimentale de l'influence de la vascularisation et de la nature du mésenchyme support dans l'évolution de la poche de Rathke de l'embryon d'oiseau. C. R. Ass. Anat. 151, 328–334 (1971)

    Google Scholar 

  • Ferrand, R.: Etude expérimentale des facteurs de la differenciation cytologique de l'adénohypophyse chez l'embryon de poulet. Arch. Biol. 3, 297–371 (1972)

    Google Scholar 

  • Franco, N., Guedenet, J.C., Grignon, G.: Ultrastructure des cellules folliculaires de l'adénohypophyse du poulet au cours de la vie embryonnaire et de la période post-natale. J. Micr. Biol. Cell. 26, 13a (1976)

  • Franks, L.M., Wilson, P.D.: Origin and ultrastructure of cells in vitro. Internat. Rev. Cytol. 48, 55–139 (1977)

    Google Scholar 

  • Frémont, P.H., Ferrand, R.: Quail Rathke's pouch differentiation. An electron microscopic study. Anat. Embryol. (Berl.) 153, 23–36 (1978)

    Google Scholar 

  • Goodyer, C.G., Hall, C.St.G., Guyda, H., Robert, F., Giroud, C.J.P.: Human fetal pituitary in culture: hormone secretion and response to somatostatin, luteinizing hormone releasing factor, thyrotropin releasing factor and dibutyryl cyclic AMP. J. Clin. Endocrinol. Metab. 45, 73–85 (1977)

    Google Scholar 

  • Grignon, G.: Développement du complexe hypothalamo-hypophysaire chez l'embryon de poulet. Nancy, Société d'impressions typographiques (1956)

    Google Scholar 

  • Grignon, G.: Aspects histophysiologiques du développement du complexe hypothalamo-hypophysaire chez l'embryon de poulet. Bull. Soc. Sc. (Nancy) 86–100 (1957)

  • Hay, E.D., Meier, S.: Glycosaminoglycan synthesis by embryonic inductors: neural tube, notochord, and lens. J. Cell Biol. 62, 889–898 (1974)

    Google Scholar 

  • Horvath, E., Kovacs, K., Penz, G., Ezrin, C.: Origin, possible function and fate of “follicular cells” in the anterior lobe of the human pituitary. An electron microscopic study. Amer. J. Pathol. 77, 199–212 (1974)

    Google Scholar 

  • Katsuyama, T., Poon, K., Spicer, S.S.: The ultrastructural histochemistry of the basement membranes of the exocrine pancreas. Anat. Rec. 188, 371–386 (1977)

    Google Scholar 

  • Kelley, R.O., Lauer, R.B.: On the nature of the external surface of cultured human embryo fibroblasts: an ultrastructural and cytochemical analysis utilising stain and lectin probes. Differentiation 3, 91–97 (1975)

    Google Scholar 

  • Kosher, R.A., Lash, J.W., Minor, R.R.: Environmental enhancement of in vitro chondrogenesis. IV Stimulation of somite chondrogenesis by exogenous chondromucoprotein. Dev. Biol. 35, 210–220 (1973)

    Google Scholar 

  • Le Douarin, N.: Etude expérimentale de l'organogenèse du tube digestif et du foie chez l'embryon de poulet. Bull. Biol. 98, 544–676 (1964)

    Google Scholar 

  • Le Douarin, N.: An experimental analysis of liver development. Med. Biol. 53, 427–455 (1975)

    Google Scholar 

  • Lehtonen, E.: Epithelio-mesenchymal interface during mouse kidney tubule induction in vivo. J. Embryol. Exp. Morphol. 34, 695–705 (1975)

    Google Scholar 

  • Lehtonen, E.: Transmission of signals in embryonic induction. Med. Biol. 54, 108–128 (1977)

    Google Scholar 

  • Le Lièvre, C., Le Douarin, N.: Etude expérimentale du développement de la glande thyroïde chez l'embryon de poulet. Anné Biol. 9, 285–291 (1970)

    Google Scholar 

  • Lippman, M.: Glycosaminoglycans and cell division. In: Epithelial-mesenchymal interactions. Williams and Wilkins, eds. (Baltimore) 208–229 (1968)

  • Mathan, M., Hermos, J.A., Trier, J.S.: Structural features of the epithelio-mesenchymal interface of rat duodenal mucosa during development. J. Cell Biol. 52, 577–588 (1972)

    Google Scholar 

  • Pastan I.H., Johnson, G.S., Anderson, W.B.: Role of cyclic nucleotides in growth control. Annu. Rev. Biochem. 44, 491–522 (1975)

    Google Scholar 

  • Pawlikowski, M., Kunert-Radek, J.: Investigations upon adenohypophysial cell proliferation in vitro: influence of oestradiol, thyrotropin releasing hormone, dibutyryl cyclic AMP and theophyl-line. Endocrinol Exp. (Bratisl.) 9, 135–140 (1975)

    Google Scholar 

  • Reynolds, S.: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 17, 208–212 (1963)

    Google Scholar 

  • Rutter, W.J., Kemp, J.D., Bradshaw, W.S., Clark, W.R., Ronzio, R.A., Sanders, T.G.: Regulation of specific protein synthesis in cytodifferentiation. J. Cell Physiol. 72, 1–18 (1968)

    Google Scholar 

  • Saxen, L., Karkinen-Jaaskelainen, M., Lehtonen, E., Nordling, S., Wartiovaara J.: Inductive tissue interactions. In: The cell surface in animal embryogenesis and development. Poste and Nicolson, eds. (New York) 331–392 (1976)

  • Schechter, J.: The ultrastructure of the stellate cells in the rabbit pars distalis. Anat. Rec. 160, 422 (1968)

    Google Scholar 

  • Schechter, J.: The ultrastructure of the stellate cells in the rabbit pars distalis. Amer. J. Anat. 126, 477–488 (1969)

    Google Scholar 

  • Schechter, J.: The cytodifferentiation of the rabbit pars distalis: an electron microscopic study. Gen. Comp. Endocrinol. 16, 1–20 (1971)

    Google Scholar 

  • Sear, C.H.J., Grant, M.E., Jackson, D.S.: Biosynthesis and release of glycoproteins by human skin fibroblasts in culture. Biochem. J. 168, 91–103 (1971)

    Google Scholar 

  • Sherer, G.K.: Tissue interaction in chick liver development: a reevaluation. I-epithelial morphogenesis: the role of vascularity in mesenchymal specificity. Dev. Biol. 46, 281–295 (1975)

    Google Scholar 

  • Spooner, B.S., Cohen, H.I., Faubion, J.: Development of the embryonic mammalian pancreas: the relationship between morphogenesis and cytodifferentiation. Dev. Biol. 61, 119–130 (1977)

    Google Scholar 

  • Stachura, M.E.: Interaction of somatostatin inhibition and dibutyryl cyclic AMP or potassium stimulation of growth hormone release from perifused rat pituitaries. Endocrinology 101, 1044–1053 (1977)

    Google Scholar 

  • Stamatoglou, S.C. Ultrastructural relationship between cell and substrate coats in serum-free and serum-supplemented cultures. J. Ultrastruct. Res. 60, 203–211 (1977)

    Google Scholar 

  • Vracko, R.: Basal lamina scaffold. Anatomy and significance for maintenance of orderly tissue structure. Amer. J. Pathol. 77, 314–346 (1974)

    Google Scholar 

  • Yaoi, Y., Kanaseki, T.: Role of microexudate carpet in cell division. Nature (London) 237, 283–285 (1973)

    Google Scholar 

  • Yaoi, Y., Onoda, T., Takahashi, H.: Inhibition of mitosis in suspension culture of chick embryo cells. Nature (London) 237, 285–286 (1972)

    Google Scholar 

  • Yoshimura, F., Soji, T., Sato, S., Yokohama, M.: Development and differentiation of rat pituitary follicular cells under normal and some experimental conditions with special reference to an interpretation of renewal cell system. Endocrinol. Jpn. 24, 435–449 (1977)

    Google Scholar 

  • Zacchei, A.M.: Lo sviluppo embrionale della quaglia giapponese (Coturnix cotunix japonica). Arch. Anat. 66, 36–62 (1961)

    Google Scholar 

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  1. Laboratoires de Biologie du Développement et de Physiologie Animale et Cellulaire, Université de Nantes, Faculté des Sciences, 2 rue de la Houssinière, F-44072, Nantes, France

    Patrick H. Frémont & Raymond Ferrand

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  1. Patrick H. Frémont
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  2. Raymond Ferrand
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This work was supported by a grant from DGRST, no 77.7.0665.

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Frémont, P.H., Ferrand, R. In vitro studies on the self-differentiating capacities of quail adenohypophysis epithelium. Anat Embryol 156, 255–267 (1979). https://doi.org/10.1007/BF00299626

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