Summary
Correlated thin-section, freeze-fracture and tracer examinations were used to examine the blood-nerve barrier of the Vater-Pacini corpuscles in cat mesentery. A laminar inner core and a multilayered outer core enfolded the terminal nerve fiber of the corpuscle. The lamellar cells of both cores were characterized by numerous vesicular membrane invaginations. Freeze-fracture images and tracer experiments employing lanthanum nitrate proved that these invaginations are static structures mediating in neither active pinocytosis nor the transcellular transport of metabolites. In both inner and outer cores, lamellar cells were connected to one another by tight junctions of either the zonula or the fascia type, that occurred between lamellar-cell processes within the lamella and between the cells of adjacent lamellae. Intravascularly applied lanthanum lay at the out-ermost regions of the corpuscles without entering their internal zones, apparently because lamellar-cell tight junctions hindered further penetration. The results of our investigations suggest strongly that the Vater-Pacini corpuscle lamellae enfolding the nerve terminal form an effective diffusion barrier against the permeation of tissue fluids, thus preserving the corpuscle internal circumference.
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References
Adrian ED, Umrath K (1929) The impulse discharge from the Pacinian corpuscle. J Physiol 68:139–154
Claude P, Goodenough DA (1973) Fracture faces of zonulae occludentes from “tight” and “leaky” epithelia. J Cell Biol 58:390–400
Farquhar MG, Palade GE (1963) Junctional complexes in various epithelia. J Cell Biol 17:375–412
Friend DS, Gilula NB (1972) Variations in tight and gap junctions in mammalian tissues. J Cell Biol 53:758–776
Gammon GD, Bronk DW (1935) The discharge of impulses from Pacinian corpuscles in the mesentery and the relation to vascular changes. Am J Physiol 114:77–84
Gotow T, Hashimoto PH (1981) Graded differences in tightness of ependymal intercellular junctions within and in the vicinity of the rat median eminence. J Ultrastruct Res 76:293–311
Gray JAB, Sato M (1955) The movement of sodium and other ions in Pacinian corpuscles. J Physiol 129:594–607
Grynszpan-Wynograd O, Nicolas G (1980) Intercellular junctions in the adrenal medulla: a comparative freeze-fracture study. Tissue Cell 12:661–672
Hull BE, Staehelin LA (1976) Functional significance of the variations in the geometrical organization of tight junction networks. J Cell Biol 68:688–704
Kristensson K, Olsson Y (1971) The perineurium as a diffusion barrier to protein tracers. Acta Neuropathol 17:127–138
Loewenstein WR (1958) Generator processes of repetitive activity in a Pacinian corpuscle. J Gen Physiol 41:825–845
Malinovský L, Berková V, Páč L (1982) The ultrastructure of axon processes in sensory corpuscles. Z Mikrosk Anat Forsch 96:844–856
Martinez-Palomo A, Erlij D (1975) Structure of tight junctions in epithelia with different permeability. Proc Natl Acad Sci 72:4487–4491
Nishi K, Oura C, Pallie W (1969) Fine structure of Pacinian corpuscles in the mesentery of the cat. J Cell Biol 43:539–552
Olsson Y, Rease TS (1971) Permeability of vasa nervorum and perineurium in mouse sciatic nerve studied by fluorescence and electron microscopy. J Neuropathol Exp Neurol 30:105–119
Pallie W, Nishi K, Oura C (1970) The Pacinian coypuscle, its vascular supply and the inner core. Acta Anat 77:508–520
Pease DC, Quilliam TA (1957) Electron microscopy of the Pacinian corpuscle. J Biophys Biochem Cytol 3:331–357
Sasaki T, Higashi S, Tachikawa T, Yoshiki S (1983) Morphology and permeability of junctional complexes in maturing ameloblasts of rat incisors. Acta Anat 116:74–83
Sato M (1961) Response of Pacinian corpuscles to sinusoidal vibration. J Physiol 159:391–409
Saxod R (1978) Development of cutaneous sensory receptors in birds. In: Jacobsen M (ed) Handbook of sensory physiology. Vol IX Springer, Berlin pp 337–417
Schiller A, Taugner R (1979) Junctions between interstitial cells of the renal medulla: A freeze-fracture study. Cell Tissue Res 203:231–240
Shinowara NL, Michel ME, Rapoport SI (1982) Morphological correlates of permability in the frog perineurium: Vesicles and “transcellular channels”. Cell Tissue Res 227:11–22
Spencer PS, Schaumburg HH (1973) An ultrastructural study of the inner core of the Pacinian corpuscle. J Neurocytol 2:217–235
Tice LW, Carter RL, Cahill MC (1977) Tracer and freeze-fracture observations on developing tight junctions in fetal rat thyroid. Tissue Cell 9:395–417
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Sakada, S., Sasaki, T. Blood-nerve barrier in the Vater-Pacini corpuscle of cat mesentery. Anat Embryol 169, 237–247 (1984). https://doi.org/10.1007/BF00315629
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DOI: https://doi.org/10.1007/BF00315629