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Fine structural survey of Gudden's tegmental nuclei in the rat: cytology and axosomatic synapses

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Summary

The fine structure of neuronal somata and axosomatic synapses in each subnucleus of Gudden's tegmental nuclei was studied by use of electron microscopy. The pars principalis of the ventral tegmental nucleus of Gudden (TVP) is composed of oblong or triangular, medium sized neurons (11.8x22.6 μm, 211.4 μm2) containing many mitochondria, lysosomes, Golgi apparatus, and rough endoplasmic reticulum composing Nissl bodies. The light oval nucleus with a prominent nucleolus is centrally situated, and indentations of its nuclear envelope are recognized in all neurons. The neuron in the pars ventralis of the dorsal tegmental nucleus of Gudden (TDV) is similar to that in the TVP, but its average size is significantly smaller (10.0x18.8 μm, 151.4 μm2), and its organelles are also less well developed. The pars dorsalis of the dorsal tegmental nucleus of Gudden (TDD) is composed of spindle shaped, small neurons (6.9x16.2 μm, 85.1 μm2) characterized by their irregular shaped nucleus with its invaginated envelope. These neurons have a thin rim of cytoplasm, poorly developed organelles and no Nissl bodies. The average number of axosomatic terminals in a sectional plane is 9.9 in the TVP, 9.6 in the TDV and 2.6 in the TDD, and the bouton covering ratio is 24.3% in the TVP, 26.5% in the TDV and 7.4% in the TDD. The respective percentages of round, flat and pleomorphic type axosomatic terminals were estimated, and the flat type terminals were found to be dominant in the TVP, the pleomorphic type terminals in the TDV, and the round type terminals in the TDD.

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References

  • Arimatsu Y, Seto A, Amano T (1981) An atlas of α-bungarotoxin binding sites and structures containing acetylcholinesterase in the mouse central nervous system. J Comp Neurol 198:603–631

    Google Scholar 

  • Bak IJ, Choi WB (1974) Electron microscopic investigation of synaptic organization of the trochlear nucleus in cat. 1 Normal ultrastructure. Cell Tissue Res 150:409–423

    Google Scholar 

  • Ban T, Zyo K (1963) Experimental studies on the mamillary peduncle and mamillotegmental tracts in the rabbit. Med J Osaka Univ 13:241–270

    Google Scholar 

  • Berman AI (1968) The brain stem of the cat. Wisconsin Univ Press, Madison 34–42

    Google Scholar 

  • Bleier R (1969) Retrograde transsynaptic cellular degeneration in mammillary and ventral tegmental nuclei following limbic decortication in rabbits of various ages. Brain Res 15:365–393

    Google Scholar 

  • Briggs TL, Kaelber WW (1971) Efferent fiber connections of the dorsal and deep tegmental nuclei of Gudden. An experimental study in the cat. Brain Res 29:17–29

    Google Scholar 

  • Boone TB, Aldes LD (1984) The ultrastructure of two distinct neuron populations in the hypoglossal nucleus of the rat. Exp Brain Res 54:321–326

    Google Scholar 

  • Card JP, Riley JN, Moore RY (1986) The motor trigeminal nucleus of the rat: Analysis of neuronal structure and the synaptic organization of noradrenergic afferents. J Comp Neurol 250:469–484

    Google Scholar 

  • Cruce JAF (1977) An autoradiographic study of the descending connections of the mammillary nuclei of the rat. J Comp Neurol 176:631–644

    Google Scholar 

  • Fallon JH, Leslie FM (1986) Distribution of dynorphin and enkephalin peptides in the rat brain. J Comp Neurol 249:293–336

    Google Scholar 

  • Falls WM, King JS (1976) The facial motor nucleus of the opossum: cytology and axosomatic synapses. J Comp Neurol 167:177–204

    Google Scholar 

  • Finley JCW, Maderdrut JL, Petrusz P (1981) The immunocytochemical localization of enkephalin in the central nervous system of the rat. J Comp Neurol 198:541–565

    Google Scholar 

  • Gobel S, Dubner R (1969) Fine structural studies of the main sensory trigeminal nucleus in the cat and rat. J Comp Neurol 137:459–494

    Google Scholar 

  • Gray EG (1959) Axo-somatic and axo-dendritic synapses of the cerebral cortex: an electron microscope study. J Anat 93:420–433

    Google Scholar 

  • Groenewegen HJ, Ahlenius S, Haber SN, Kowall NW, Nauta WJH (1986) Cytoarchitecture, fiber connections, and some histochemical aspects of the interpeduncular nucleus in the rat. J Comp Neurol 249:65–102

    Google Scholar 

  • Hayakawa T, Seki M, Zyo K (1981) Studies on the efferent projections of the interpeduncular complex in cats. Okajimas Folia Anat Jpn 58:1–16

    Google Scholar 

  • Hayakawa T, Zyo K (1982) Organization of the habenulo-interpeduncular connections in cats: A horseradish peroxidase study. Brain Res 240:3–11

    Google Scholar 

  • Hayakawa T, Zyo K (1984) Comparative cytoarchitectonic study of Gudden's tegmental nuclei in some mammals. J Comp Neurol 216:233–244

    Google Scholar 

  • Hayakawa T, Zyo K (1984) Comparative anatomical study of the tegmentomammillary projections in some mammals: A horseradish peroxidase study. Brain Res 300:335–349

    Google Scholar 

  • Hayakawa T, Zyo K (1985) Afferent connections of Gudden's tegmental nuclei in the rabbit. J Comp Neurol 235:169–181

    Google Scholar 

  • Hayakawa T, Zyo K (1986) Subcortical afferents to the nucleus reticularis tegmenti pontis in the rabbit: A retrograde horseradish peroxidase study. Okajimas Folia Anat Jpn 63:159–178

    Google Scholar 

  • Herkenham M, Nauta WJH (1979) Efferent connections of the habenular nuclei in the rat. J Comp Neurol 187:19–48

    Google Scholar 

  • Hunt S, Schmidt J (1978) Some observations of the binding patterns of α-bungarotoxin in the central nervous system of the rat. Brain Res 157:213–232

    Google Scholar 

  • Ide LS, Killackey HP (1985) Fine structural survey of the rat's brainstem sensory trigeminal complex. J Comp Neurol 235:145–168

    Google Scholar 

  • Irle E, Markowitsch HJ (1982) Connections of the hippocampal formation, mammillary bodies, anterior thalamus and cingulate cortex. A retrograde study using horseradish peroxidase in the cat. Exp Brain Res 47:79–94

    Google Scholar 

  • Irle E, Sarter M, Guldin WO, Markowitsch HJ (1984) Afferents to the ventral tegmental nucleus of Gudden in the mouse, rat and cat. J Comp Neurol 228:509–541

    Google Scholar 

  • Kubota Y, Inagaki S, Shiosaka S, Cho HJ, Tateishi K, Hashimoto E, Hamaoka T, Tohyama M (1983) The distribution of cholecystokinin octapeptide-like structures in the lower brain stem of the rat: An immunohistochemical analysis. Neuroscience 9:587–604

    Google Scholar 

  • Langford LA, Coggeshall RE (1980) The use of potassium ferricyanide in neural fixation. Anat Rec 197:297–303

    Google Scholar 

  • Liu R, Chang L, Wickern G (1984) The dorsal tegmental nucleus: An axoplasmic transport study. Brain Res 310:123–132

    Google Scholar 

  • Marchand ER, Riley JN, Moore RY (1980) Interpeduncular nucleus afferents in the rat. Brain Res 193:339–352

    Google Scholar 

  • Morest DK (1961) Connexions of the dorsal tegmental nucleus in rat and rabbit. J Anat 95:229–246

    Google Scholar 

  • Nakamura Y (1975) An electron microscope study of the red nucleus in the cat, with special reference to the quantitative analysis of the axosomatic synapses. Brain Res 94:1–17

    Google Scholar 

  • Nauta WJH (1958) Hippocampal projections and related neural pathways to the midbrain in the cat. Brain 81:319–341

    Google Scholar 

  • Niimi K, Koizuka M, Kawamura S, Abe K (1972) Efferent projections of the mamillary body in the cat. Okajimas Folia Anat Jpn 49:129–156

    Google Scholar 

  • Ottersen OP, Storm-Mathisen J (1984) Neurons containing or accumulating transmitter amino acids. In: Björklund A, Hökfelt T, Kuhar MJ (eds) Handbook of chemical neuroanatomy, vol 3. Elsevier, Amsterdam New York Oxford 141–246

    Google Scholar 

  • Paxinos G, Butcher LL (1985) Organizational principles of the brain as revealed by cholin acetyltransferase and acetylcholinesterase distribution and projections. In: Paxinos G (ed) The rat nervous system. vol 1. Forebrain and midbrain. Academic Press, Sydney 487–522

    Google Scholar 

  • Petrovický P (1971) Structure and incidence of Gudden's tegmental nuclei in some mammals. Acta Anat 80:273–286

    Google Scholar 

  • Petrovický P (1973) Note on the connections of Gudden's tegmental nuclei. 1. Efferent ascending connections in the mamillary peduncle. Acta Anat 86:165–190

    Google Scholar 

  • Pickel VM, Sumal KK, Reis DJ, Miller RJ, Hervonen A (1980) Immunohistochemical localization of enkephalin and substance P in the dorsal tegmental nuclei in human fetal brain. J Comp Neurol 193:805–814

    Google Scholar 

  • Rotter A, Birdsall NJM, Field PM, Raisman G (1979) Muscarinic receptors in the central nervous system of the rat. II. Distribution of binding of [3H]propylbenzilylcholine mustard in the midbrain and hindbrain. Brain Res Rev 1:167–183

    Google Scholar 

  • Shibata H, Suzuki T (1984) Efferent projections of the interpeduncular complex in the rat, with special reference to its subnuclei: A retrograde horseradish peroxidase study. Brain Res 296:345–349

    Google Scholar 

  • Shibata H, Suzuki T, Matsushita M (1986) Afferent projections to the interpeduncular nucleus in the rat, as studied by retrograde and anterograde transport of wheat germ aggulutinin conjugated to horseradish peroxidase. J Comp Neurol 248:272–284

    Google Scholar 

  • Smaha LA, Kaelber WW (1973) Efferent fiber projections of the habenula and the interpeduncular nucleus. An experimental study in the opossum and cat. Exp Brain Res 16:291–308

    Google Scholar 

  • Spencer RF, Sterling P (1977) An electron microscope study of motoneurons and interneurons in the cat abducens nucleus identified by retrograde intraaxonal transport of horseradish peroxidase. J Comp Neurol 176:65–86

    Google Scholar 

  • Tredici G, Pizzini G, Milanesi S (1976) The ultrastructure of the nucleus of the oculomotor nerve (somatic efferent portion) of the cat. Anat Embryol 149:323–346

    Google Scholar 

  • Uchizono K (1965) Characteristic of excitatory and inhibitory synapses in the central nervous system in the cat. Nature 207:642–643

    Google Scholar 

  • Veazey RB, Amaral DG, Cowan WM (1982) The morphology and connections of the posterior hypothalamus in the cynomolgus monkey (Macaca fascicularis). II. Efferent connections. J Comp Neurol 207:135–156

    Google Scholar 

  • Westman J (1971) Quantitative estimation of the proportion of perikaryal surface area covered with boutons, a possibility to distinguish different nerve cell populations. Brain Res 32:203–207

    Google Scholar 

  • Westrum LE, Black R (1971) Fine structural aspects of the synaptic organization of the spinal trigeminal nucleus (pars interpolaris) of the cat. Brain Res 25:265–287

    Google Scholar 

  • Yamazoe M, Shiosaka S, Emson PC, Tohyama M (1985) Distribution of neuropeptide Y in the lower brain stem: An immunohistochemical analysis. Brain Res 335:109–120

    Google Scholar 

  • Zyo K, Hayakawa T, Seki M, Ryu T (1978) Caudal projections of the habenular nuclei in cats: Fibers from the lateral habenular nucleus. Med J Osaka Univ 28:229–243

    Google Scholar 

Download references

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  1. Department of Anatomy, Hyogo College of Medicine, Mukogawa-cho, 663, Nishinomiya, Hyogo, Japan

    T. Hayakawa & K. Zyo

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  1. T. Hayakawa
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  2. K. Zyo
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Hayakawa, T., Zyo, K. Fine structural survey of Gudden's tegmental nuclei in the rat: cytology and axosomatic synapses. Anat Embryol 177, 485–493 (1988). https://doi.org/10.1007/BF00305136

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