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Cationic glycoproteins in sera of patients with acute infections identified as kappa light chain glycosylated IgG

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Abstract

In half of the sera from patients with acute bacterial infections and 15% of the sera from patients with acute viral infections glycoproteins were found that form a scalariform pattern in the cationic range upon isoelectric focusing. The cationic glycoproteins appeared with the clinical illness. After subsidence of the symptoms they disappeared within 4 to 6 weeks. The proteins were identified as immunoglobulin G (IgG) by determination of the NH2-terminal amino acid sequence. Remarkably, these IgG only contained light chains of the kappa type with high proportions of carbohydrates. Both, N-glycosidic- and O-glycosidic-bound glycans were present. The glycosylated light chains may render the cationic IgG multireactive. Thus, it may be part of an early nonspecific immune defense mechanism.

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Abbreviations

aa:

amino acid

IEF:

isoelectric focusing

IgG:

immunoglobulin G

Ig/H:

immunoglobulin heavy chain

Ig/L:

immunoglobulin light chain

M r :

relative molecular mass

PAGE:

polyacrylamide gelelectrophoresis

PBS:

phosphate-buffered saline

PVDF:

polyvinylidenedifluoride

SDS:

sodium dodecylsulfate

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Authors and Affiliations

  1. Hygiene-Institut der Universität, Abteilung Medizinische Mikrobiologie, Kreuzbergring 57, D-37075, Göttingen, Germany

    Klaus Ritter, Antje Wiebusch, Helmut Eiffert & Reiner Thomssen

  2. Neurologische Klinik der Universität, D-37075, Göttingen, Germany

    Michael Mäder

  3. Max-Planck-Institut für experimentelle Medizin, D-37075, Göttingen, Germany

    Hartmut Kratzin

Authors
  1. Klaus Ritter
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  2. Antje Wiebusch
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  3. Michael Mäder
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  4. Helmut Eiffert
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  5. Hartmut Kratzin
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  6. Reiner Thomssen
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Ritter, K., Wiebusch, A., Mäder, M. et al. Cationic glycoproteins in sera of patients with acute infections identified as kappa light chain glycosylated IgG. Med Microbiol Immunol 182, 107–117 (1993). https://doi.org/10.1007/BF00190263

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  • DOI: https://doi.org/10.1007/BF00190263

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