Summary
The preparation of the complement-fixing antigen of Respiratory syncytial virus (4 strains) from established cell lines (5 cell lines) is described. The following problems are discussed:
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1.
Determination of antigenic character of established cell lines.
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2.
Characterization of anticell titres from animal RS-antisera.
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3.
Comparative examination of different RS-virusantigens prepared on different established cell lines.
The best results were obtained with the cell linesHelsi andFl in combination with the virus strainsRandall andLong.
Zusammenfassung
Es werden Untersuchungen zur Gewinnung komplement-bindender RS-Virus-Antigene vorgelegt. Drei Problemstellungen wurden bearbeitet:
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1.
Das antigenetische Verhalten von Permanentzellstämmen.
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2.
Die Bestimmung von Antizelltitern in RS-Antiseren.
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3.
Die vergleichende Prüfung verschiedener RS-Virus-Antigene, die auf unterschiedlichen Zellstämmen präpariert wurden.
Die günstigsten Resultate bezüglich der Präparation eines komplementbindenden RS-Antigens konnten bei Verwendung der ZellstämmeHelsi undFl in Kombination mit den VirusstämmenRandall undLong erzielt werden.
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Unserem verehrten Lehrer, Herrn OMR Prof. Dr. Urbach, zum 60. Geburtstag gewidmet.
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Brunnemann, H., Stelzner, A. & Hartmann, M. über das antigenetische Verhalten verschiedener Permanentzellstämme sowie über deren unterschiedliche Verwendbarkeit für die Gewinnung eines komplementbindenden Respiratory syncytial virus-Antigens. Z. med. Mikrobiol. u. Immunol. 155, 275–282 (1970). https://doi.org/10.1007/BF02275113
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DOI: https://doi.org/10.1007/BF02275113