Abstract
A technique using antibody-coated tubes allows the radioimmunological determination of tetanus antibodies in a simple and rapid way. The procedure is based on the competition for125I-tetanus toxin between the insolubilized antibodies and the antibodies present in the unknown sample. When antibodies and125I labeled tetanus toxin are incubated in the antibody-coated polyethylene vessel the detection limit is about 0.016 IU/ml. When tetanus toxin is preincubated together with its antibody in uncoated vessels before the mixture is transferred to antibody-coated vessels, the sensitivity can be raised about tenfold.
The method is especially useful for screening multiple samples.
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Bernáth, S., Habermann, E. Solid-phase radioimmunoassay in antibody-coated tubes for the quantitative determination of tetanus antibodies. Med Microbiol Immunol 160, 47–51 (1974). https://doi.org/10.1007/BF02124342
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DOI: https://doi.org/10.1007/BF02124342