Skip to main content
SpringerLink
Log in

Use of the enzyme-linked immunosorbent assay (ELISA) for the estimation of serum antibodies in an influenza virus vaccine study

  • Published:
Medical Microbiology and Immunology Aims and scope Submit manuscript

Abstract

The value of the enzyme-linked immunosorbent assay (ELISA) for de termining the serum antibody responses of volunteers following immunisation with various inactivated influenza virus vaccines was assessed, and the incidence of seroconversions, as measured by both haemagglutination-inhibition (HI) and ELISA response of the volunteers determined. ELISA was found to be more sensitive than the HI test in detecting serum antibodies, but was also less specific under the conditions used. With regard to efficacy, the whole virus vaccine proved to be more effective in inducing serum antibody in an unprimed population than either tween-ether split or subunit adsorbed vaccines, but the reverse situation held when the population was primed with respect to the antigen concerned.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Avrameas S (1969) Coupling of enzymes to proteins with glutaraldehyde use of the conjugate for the detection of antigens and antibodies. Immunochem 6:43–52

    PubMed  Google Scholar 

  • Aymard-Henry M, Coleman MT, Dowdle WR, Laver WG, Webster RG (1973) Influenza virus neuraminidase and neuraminidase-inhibition test procedures. Bull Wld Hlth Org 48:199–202

    Google Scholar 

  • Bishai FR, Galli R (1978) Enzyme-linked immunosorbent assay for detection of antibodies to influenza A and B parainfluenza type 1 in sera of patients. J Clin Microbiol 8:648–656

    PubMed  Google Scholar 

  • Brady M, Furminger IGS (1976) A surface antigen influenza vaccine. 1. Purification of haemagglutinin and neuraminidase proteins. J Hyg (Camb) 77:161–172

    Google Scholar 

  • Eastwood LM, Jennings R, Milner RDG, Potter CW (1979) Reactogenicity and immunogenicity of a surface-antigen-adsorbed influenza virus vaccine in children. J Clin Path 32:534–537

    PubMed  Google Scholar 

  • Expert Committee on Respiratory Virus Diseases (1959) World Health Organisation Technical Report Number 170. World Health Organisation, Geneva

    Google Scholar 

  • Gilman SC, Docherty JS (1977) Detection of antibodies specific for herpes simplex virus in human sera by the enzyme-linked immunosorbent assay. J Inf Dis Supp 136:S286-S293

    Google Scholar 

  • Gross PA, Davis AE (1979) Neutralisation test in influenza: Use in individuals without haemagglutination inhibition antibody. J Clin Microbiol 10:382–384

    PubMed  Google Scholar 

  • Hobson D, Curry RL, Beare AS, Ward-Gardner A (1972) The role of serum haemagglutination-inhibiting antibody in protection against challenge infection with influenza A2 and B viruses. J Hyg (Camb) 70:767–777

    Google Scholar 

  • Jennings R, Clark A, Oxford JS, Hockley D, Potter CW (1978) Reactogenicity and immunogenicity of whole and ether-tween split influenza A virus vaccines in volunteers. J Infect Dis 138:577–586

    PubMed  Google Scholar 

  • Jennings R, Denton MD, Potter CW (1976) The hamster as an experimental animal for the study of influenza. I. The role of antibody in protection. Med Microbiol Immunol 162:217–226

    PubMed  Google Scholar 

  • Jennings R, Potter CW, Massey PMO, Duerden BI, Martin J, Bevan AM (1981) Responses of volunteers to inactivated influenca virus vaccines. J Hyg (Camb) 86:1–16

    Google Scholar 

  • Kilbourne ED (1978) Influenza as a problem in immunology. J Immunol 120:1447–1452

    PubMed  Google Scholar 

  • Lambre C, Kasturi KN (1979) A microplate immunoenzyme assay for anti-influenza antibodies. J Immunol Meth 26:61–67

    Google Scholar 

  • Leinikki PO, Shekarchi I, Tzan N, Madden DL, Sever JL (1979) Evaluation of enzyme-linked immunosorbent assay (ELISA) for mumps virus antibodies. Proc Soc Exp Biol Med 160:363–367

    PubMed  Google Scholar 

  • Magee JT (1980) An enzyme labelled immunosorbent assay for Brucella abortus antibodies. J Med Microbiol 13:167–172

    PubMed  Google Scholar 

  • Meiklejohn G, Kempe CH, Thalman WG, Lennette EH (1952) Evaluation of monovalent influenza vaccines. II. Observations during an influenza A-prime epidemic. Amer J Hyg 55:12–21

    PubMed  Google Scholar 

  • Mostow SR, Schild GC, Dowdle WR, Wood JR (1975) Application of the single radial diffusion test for assay of antibody to influenza type A viruses. J Clin Microbiol 2:531–541

    PubMed  Google Scholar 

  • Murphy BR, Holley HP Jr, Berquist EJ, Levine MM, Spring SB, Maassab HF, Kendal AP, Chanock RM (1979) Cold-adapted variants of influenza A virus: Evaluation in adult sero-negative volunteers of A/Scotland/840/74 and A/Victoria/3/75 cold-adapted recombinants derived from the cold-adapted A/Ann Arbor/6/60/strain. Inf Imm 23:253–259

    Google Scholar 

  • Pandemic Working Group of the Medical Research Council (United Kingdom) Committee on Influenza and Other Respiratory Virus Vaccines (1977) Antibody responses and reactogenicity of graded doses of inactivated influenza A/New Jersey/76 whole-virus vaccine in humans. J Infect Dis 136:SuppI S475-S483

    Google Scholar 

  • Parker JC, O'Beirne AJ, Collins MJ Jr (1979) Sensitivity of enzyme-linked immunosorbent assay, complement fixation and haemagglutination inhibition serological tests for detection of Sendai virus antibody in laboratory mice. J Clin Microbiol 9:444–447

    PubMed  Google Scholar 

  • Parkman PD, Galasso GJ, Top FH Jr, Noble GR (1976) Summary of clinial trials of influenza vaccines. J Infect Dis 134:100–107

    Google Scholar 

  • Potter CW, Clark A, Jennings R, Schild GC, Wood JM, McWilliam PKA (1980) Reactogenicity and immunogenicity of inactivated influenza A (HINI) virus vaccine in unprimed children. J Biol Stand 8:35–48

    PubMed  Google Scholar 

  • Richardson LS, Yolken RH, Beshe RB, Camargo E, Kim HW, Chanock RM (1978) Enzyme-linked immunosorbent assay for measurement of serological response to respiratory syncytial virus infection. Inf Imm 20:660–664

    Google Scholar 

  • Schild GC, Pereira MS, Chakraverty P (1975) Single radial heamolysis: a new method for the assay of antibody to influenza haemagglutinin. Bull Wld Hlth Org 52:43–50

    Google Scholar 

  • Schild GC, Stuart-Harris CH (1976) The molecular virology and replication of influenza viruses. In “Influenza: The Viruses and the Disease”. Edward Arnold (Publishers) London, pp 22–50

    Google Scholar 

  • Sever JL (1962) Application of a microtechnique to viral serological investigations. J Immunol 88:320–329

    PubMed  Google Scholar 

  • Six HR, Kasel JA (1978) Radioimmunoprecipitation assay for quantitation of serum antibody to the haemagglutinin of type A influenza virus. J Clin Microbiol 7:165–171

    PubMed  Google Scholar 

  • Vejtorp M (1978) Enzyme-linked immunosorbent assay for determination of rubella 1gG antibodies. Acta Path Microbiol Scand 86:387–392

    Google Scholar 

  • Wisdom GB (1976) Enzyme immunoassay. Clin Chem 22:1243–1255

    PubMed  Google Scholar 

  • Wood JM, Schild GC, Newman RW, Seagroatt V (1977) An improved single radial immunodiffusion technique for the assay of influenza haemagglutinin antigen. Application for potency determination of inactivated whole virus and subunit vaccines. J Biol Stand 5:237–247

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Depcrtment of Virology, Academic Division of Pathology, University of Sheffield School of Medicine, Beech Hill Road, S 10 2RX, Sheffield, England

    R. Jennings, T. Smith & C. W. Potter

Authors
  1. R. Jennings
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. T. Smith
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. C. W. Potter
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jennings, R., Smith, T. & Potter, C.W. Use of the enzyme-linked immunosorbent assay (ELISA) for the estimation of serum antibodies in an influenza virus vaccine study. Med Microbiol Immunol 169, 247–258 (1981). https://doi.org/10.1007/BF02125524

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02125524

Keywords

Search

Navigation