Abstract
Cytotoxic effects of a leukocidin from Fusobacterium necrophorum were demonstrated on bovine hepatic cells. The cytotoxic response was dose-dependent and could be inhibited by homologous antiserum. Scanning electron microscopy revealed damaged hepatic cell surface. These findings indicated a pathogenic role of the leukocidin in F. necrophorum infections.
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References
Aalbaek B (1971) Sphaerophorus necrophorus a study of 23 strains. Acta Vet Scand 12:344–364
Coyle-Dennis IE, Lauerman LH (1979) Correlations between leukocidin production and virulence of two isolates of Fusobacterium necropharum. Am J Vet Res 40:274–276
Donta ST, Shaffer SJ (1980) Effects of Clostridium difficile toxin on tissue-cultured cells. J Inf Dis 141:218–221
Fales WH, Warner IF, Teresa GW (1977) Effects of Fusobacterium necrophorum leukocidin on rabbit peritoneal macrophages in vitro. Am J Vet Res 38:491–495
Gladstone GP, van Heyningen WE (1951) Staphylococcal leukocidin. Brit J Exp Pathol 38:123–127
Kessel RW, Braum W (1965) Cytotoxicity of endotoxin in vitro. Effects on macrophages from normal guinea pig. Aust J Exp Biol Med Sci 43:511–522
Kanoe M, Iriki M (1985) Effects of Fusobacterium necrophorum haemolysin on peripheral leukocytes in vitro. FEMS Microbiol Lett 28:187–191
Kanoe M, Iwaki K (1986) Adherence of Fusobacterium necrophorum to bovine hepatic cells. FEMS Microbiol Lett 35:245–248
Kanoe M, Imagawa H, Toda M, Sato A, Inoue M, Yoshimoto Y (1976) Bacteriology of bovine hepatic abscesses. Jpn J Vet Sci 38:263–268
Kanoe M, Ishii T, Mizutani K, Blobel H (1986) Partial characterization of leukocidin from Fusobacterium necrophorum. Zbl Bakt Hyg A 261:170–176
Roberts DS (1967) The pathogenic synergy of Fusiformis necrophorus and Corynebacterium pyogenes. Influence of the leukocidal exotoxin of F. necrophorus. Brit J Exp Path 48:665–673
Scanlan CM, Berg JN, Fales WH (1982) Comparative in vitro leukotoxin production of three bovine strains of Fusobacterium necrophorum. Am J Vet Res 43:1329–1333
Simon PC, Stovell PL (1969) Diseases of animals associated with Sphaerophorus necrophorus: Characteristics of the organism. Vet Bull 39:311–315
Tarnvik A, Sundqvist G, Gothefors L, Gustafsson H (1986) Meningitis caused by Fusobacterium necrophorum. eur J Clin Microbiol 5:353–355
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Ishii, T., Kanoe, M., Inoue, T. et al. Cytotoxic effects of a leukocidin from Fusobacterium necrophorum on bovine hepatic cells. Med Microbiol Immunol 177, 27–32 (1987). https://doi.org/10.1007/BF00190308
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DOI: https://doi.org/10.1007/BF00190308