Abstract
The origin of urinary enzymes is obscure. We therefore determined the levels of urinary, serum and kidney enzymes (lysozyme, amylase, alkaline phosphatase, glutamate transaminase, lactic dehydrogenase, leucine aminopeptidase and β-glucuronidase) and isozymes (alkaline phosphatase, glutamate transaminase, leucine aminopeptidase and β-glucuronidase) in Wistar rats with nephrotoxin nephritis or aminonucleoside nephrosis.
The urinary enzyme activities varied in several ways in rats with nephrotoxin nephritis and with aminonucleoside nephrosis. A significant difference in the isozymes was found in the two groups. The isozyme-pattern of urinary enzymes resembles that of kidney enzymes, but not that of serum enzymes.
These findings indicate that the urinary enzymes are mainly formed by destruction and renewal of renal tissue.
Zusammenfassung
Es wurden Urin-, Serum-und Nierenenzyme von Wistarratten nach experimenteller Nierenschädigung untersucht und die Aktivitäten von 6 Urinenzymen (Lysozym, alkalische Phosphatase, Glutamin-Oxalacetat-Transaminase, Lactat-Dehydrogenase, Leucin-Aminopeptidase und β-Glucuronidase) bestimmt und gleichzeitig auch Isoenzyme (alkalische Phosphatase, Glutamin-Oxalacetat-Transaminase und β-Glucuronidase) untersucht.
Wir finden im Aktivitätsverlauf der Urinenzyme und der Isoenzyme deutliche Unterschiede zwischen den Ratten, denen Nephrotoxin und denen Aminonucleosid injiziert wurde. Bei den Isoenzymen der verschiedenen Urinenzyme war bei beiden Gruppen ein deutlicher Unterschied zu beobachten. Das Muster der Isoenzyme ist bei Urin-und Nierenfermenten ähnlich, weicht aber von dem der Serumfermente ab.
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Miyao, M., Hasegawa, Y., Kochi, T. et al. Die Enzymausscheidung im Urin. Z. Kinder-Heilk. 112, 124–132 (1972). https://doi.org/10.1007/BF00443499
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DOI: https://doi.org/10.1007/BF00443499