Summary
A total of 30 inbred Wistar rats were orally administered 70 μg/ml solution of N-methyl-N′-nitro-N-nitrosoguanidine (MNNG) for 35 weeks and then tap water for the following 20 to 30 weeks. Four of the 20 females and two of ten males developed carcinoids in the glandular stomach, but no metastasis could be found. Carcinoids developed most frequently in the fundic portion along the greater curvature. Histologically, these tumors were medullary anaplastic carcinomas containing two different endocrine cell populations. The first cell type was argentaffin having electron-dense, somewhat pleomorphic secretory granules (437–810 nm) and the second type was argyrophil having round granules with a dense core and a pale halo (550 nm). None of these tumors showed endocrine immunoactivity for gastrin, somatostatin, insulin, glucagon, and enkephalin. One of these gastric tumors developed into scirrhous carcinoma, but differentiated adenocarcinoma could not be seen in the glandular stomach.
Zusammenfassung
Durch orale Applikation von MNNG (N-methyl-N′-nitro-N-nitrosoguanidin) wurden Carcinoide im Drüsenmagen von Ratten erzeugt. Dreißig Wistar-Ratten wurde eine 70 μg/ml Lösung von MNNG 35 Wochen lang verabreicht, dann erhielten die Tiere 20–30 Wochen lang Leitungswasser. Bei vier von 20 weiblichen und zwei von zehn männlichen Tieren wurden Carcinoide im Drüsenmagen gefunden, Metastasen wurden nicht beobachtet. Die Tumoren waren vorwiegend im Fundusbereich entlang der großen curvatur lokalisiert. Histologisch zeigten die Carcinoide medullär-anaplastische Strukturen, die aus zwei Typen endokriner Zellen bestanden. Der erste Typ war Argentaffin und hatte elektronendichte, mäßig irreguläre Sekretgranula (437–810 nm); der zweite zeigte eine argyrophile Reaktion und hatte runde Sekretgranula mit elektronendichtem Core unter Ausbildung eines Halo (550 nm). Immunhistologische Untersuchungen (PAP-Methode) ergaben keine positiven Reaktionen am Zytoplasma der Tumorzellen gegenüber Antiseren von Gastrin, Insulin, Glucagon und Enkephalin. Einer dieser endokrinen Tumoren entwickelte sich weiter zum scirrhösen Carcinom; ein differenziertes Adenocarcinom des Drüsenmagens wurde nicht festgestellt.
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Tahara, E., Ito, H., Nakagami, K. et al. Induction of carcinoids in the glandular stomach of rats by N-methyl-N′-nitro-N-nitrosoguanidine. J Cancer Res Clin Oncol 100, 1–12 (1981). https://doi.org/10.1007/BF00405896
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DOI: https://doi.org/10.1007/BF00405896