Summary
G2-enriched fractions of Ehrlich ascites tumor cells (up to 80%–85% G2 cells) separated from anaerobically and aerobically cultured asynchronous populations by centrifugal elutriation revealed the same growth characteristics after recultivation under standard conditions: a significant proportion of cells with increased DNA (DNA content>4C) emerged. Interruption of DNA synthesis by deprivation of oxygen may account for polyploidisation (over-replication) of DNA but other mechanisms must be taken into consideration.
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Kroll, C., Schneider, F. Polyploidisation of G2M phase cells separated from aerobically and anaerobically grown Ehrlich ascites tumor cells. J Cancer Res Clin Oncol 113, 298–300 (1987). https://doi.org/10.1007/BF00396389
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DOI: https://doi.org/10.1007/BF00396389