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Hydrocortisone-induced accumulation of fibronectin mRNA and cell surface-associated fibronectin

  • Original Papers
  • Experimental Oncology
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Summary

A Morris hepatoma 7777-derived cell line, DTH-3, was used to study the control of fibronectin gene expression. In cultures of DTH-3 cells in conventional medium supplemented with serum or in chemically defined MX-83 medium supplemented with insulin no cell surface fibronectin was detectable by indirect immunofluorescence techniques using specific polyclonal antibodies. By Northern blot hybridization analysis of dose- and time-dependent accumulation of 8 kb fibronectin mRNA in response to hydrocortisone treatment was demonstrated. Furthermore, 24 h after addition of hydrocortisone an extensive fibrillar fibronectin network was established. The results suggest that the hydrocortisone-dependent induction of fibronectin production might, at least in part, be controlled at the transcriptional level.

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Authors and Affiliations

  1. Fratienhofer Institut für Toxikologie, Nikolai-Fuchs-Strasse 1, D-3000, Hannover 61, Federal Republic of Germany

    Martin Begemann

  2. Medizinische Abteilung des Silikose-Forschungsinstituts der Bergbau-Berufsgenossenschaft Bochum, Hundscheidtstrasse 12, D-4630, Bochum 1, Federal Republic of Germany

    Bruno Voss

  3. Abteilung für Allgemeine Toxikologie, Hamburg University, Medical School, Grindelallee 117, D-2000, Hamburg 13, Federal Republic of Germany

    Dieter Paul

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  2. Bruno Voss
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Begemann, M., Voss, B. & Paul, D. Hydrocortisone-induced accumulation of fibronectin mRNA and cell surface-associated fibronectin. J Cancer Res Clin Oncol 114, 477–481 (1988). https://doi.org/10.1007/BF00391495

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  • DOI: https://doi.org/10.1007/BF00391495

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