Abstract
Monoclonal antibody (mAb) uptake in metastatic lung lesions was depicted and evaluated by digital autoradiography. The models examined were experimental metastases of a human melanoma in nude mice and spontaneous metastases of human melanoma in immunocompromized young rats. By comparing uptake patterns in local (s.c.) tumours and in lung processes of various sizes it was found that patterns were essentially similar in both types of malignant tissue. From the point of view of visualization, however, the high blood content of lung tissue resulted in high background and low contrast. This could be overcome by the use of rapidly cleared antibody fragments.
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References
Bailly M, Doré J-F (1991) Human tumor spontaneous metastasis in immunosuppressed newborn rats: II. Multiple selections of human melanoma metastatic clones and variants. Int J Cancer 49:750–757
Bailly M, Doré J-F (1992) Clonal drift and role of chromosome dosage in human melanoma metastatic cell lines: a statistical analysis. Anticancer Res (in press)
Beatty JD, Duda BB, Williams LE, Sheibani K, Paxton RJ, Beatty BG, Philben VJ, Werner JL, Shively JE, Vlahos WG, Kokal WA, Riihimaki DU, Terz JJ, Wagman LD (1986) Preoperative imaging of colorectal carcinoma with 111In-labeled anti-carcinoembryonic antigen monoclonal antibody. Cancer Res 46:6494–6502
Cobb LM (1989) Intratumour factors influencing the access of antibody to tumour cells. Cancer Immunol Immunother 28:235–240
Fodstad O, Aamdal S, McMenamin M, Nesland JM, Pihl A (1988) A new experimental metastasis model in athymic nude mice, the human malignant melanoma LOX. Int J Cancer 41:442–449
Fraker PJ, Speck JC (1980) Protein and cell membrane iodinations with a sparingly soluble chloroamide, 1,3,4,6-tetrachloro-3α,6α-diphenylglycoluril. Biochem Biophys Res Commun 80:849–857
Fujimori K, Covell DG, Fletcher JE, Weinstein JN (1989) Modeling analysis of the global and microscopic distribution of immunoglobulin G, F(ab′)2, and Fab in tumors. Cancer Res 49:5656–5663
Jain RK (1990) Physiological barriers to delivery of monoclonal antibodies and other macromolecules in tumors. Cancer Res 50 [Suppl]:814s-819s
Jirtle RL (1988) Chemical modification of tumour blood flow. Int J Hyperthermia 4:355–371
Le Doussal J-M, Gautherot E, Martin M, Barbet J, Delaage M (1991) Enhanced in vivo targeting of an asymmetric bivalent hapten to double-antigen-positive mouse B cells with monoclonal antibody conjugate cocktails. J Immunol 146:169–175
Mach J-P, Carrel S, Merenda C (1974) In vivo localization of radiolabeled antibodies to carcinoembryonic antigen in human colon carcinoma grafted into nude mice. Nature 248:704–706
Matzku S, Schmid J, Tilgen W (1990) Autoradiographic evaluation of monoclonal antibodies' access to melanoma-associated antigens in melanoma xenografts. J Invest Dermatol 95:671–676
Reinhold HS, Zurcher C, Van den Berg-Blok AE (1990) Differential heat sensitivity of tumour microvasculature. Eur J Cancer 26:541–543
Schmid U, Bihl H, Matzku S (1990 a) Antibody accumulation in small tissue samples: assessment by quantitative autoradiography. Nucl Med Biol 17:585–596
Schmid U, Schirrmacher V, Bihl H, Matzku S (1990 b) Quantitation of antibody uptake in spontaneous metastases. Br J Cancer 62:64–66
Sevick EM, Jain RK (1989) Viscous resistance to blood flow in solid tumors: effect of hematocrit on intratumor blood viscosity. Cancer Res 49:3513–3519
Sharkey RM, Filion D, Fand I, Primus FJ, Goldenberg DM (1986) A human colon cancer metastasis model for radioimmunodetection. Cancer Res 46:3677–3683
Stickney DR, Anderson LD, Slater JB, Ahlem CN, Kirk GA, Schweighardt SA, Frincke JM (1991) Bifunctional antibody: a binary radiopharmaceutical delivery system for imaging colorectal carcinoma. Cancer Res 51:6650–6655
Suter L, Bröcker EB, Brüggen J, Ruiter DJ, Sorg C (1983) Heterogeneity of primary and metastatic human malignant melanoma as detected with monoclonal antibodies in cryostat sections of biopsies. Cancer Immunol Immunother 16:53–58
Tilgen W, Matzku S (1990) Antibody-mediated modulation of melanoma-associated antigens: access of monoclonal antibodies into melanoma cells and melanoma tissue. In: Ferrone S (ed) Human melanoma: from basic research to clinical applications. Springer, Heidelberg, Berlin, New York, pp 119–132
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Schmid, U., Bailly, M., Fodstad, O. et al. Monoclonal antibody accumulation in experimental and spontaneous lung metastases of human malignant melanoma in rodents. J Cancer Res Clin Oncol 119, 342–345 (1993). https://doi.org/10.1007/BF01208842
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DOI: https://doi.org/10.1007/BF01208842