Zusammenfassung
Einhundertzwanzig männlichen Sprague-Dawley-Ratten wurden jeweils 3000 Zellen eines Yoshida-Sarkoms in die Wand des Colon descendens verimpft. Am 8. Tag nach der Tumorimplantation wurden die Tiere randomisiert in vier Versuchsgruppen von jeweils 30 Ratten aufgeteilt. Die Wirkung von Cyclophosphamid (70 mg/kg), BCNU (25 mg/kg) und Methyl-CCNU (45 mg/kg) bei einmaliger i.p. Gabe wurde untersucht. Das ins Colon transplantierte Yoshida-Sarkom ist gegeüber allen drei Chemotherapeutika sensibel. Bei den gewählten Dosierungen zeigte Cyclophosphamide die günstigsten Ergebnisse. Die beiden Nitrosoharnstoffe waren in ihrer Antitumoraktivität vergleichbar, während bei Methyl-CCNU die toxischen Wirkungen sich als ausgeprägter erwiesen.
Der Einsatz des Modells im Rahmen der tierexperimentellen Prüfung neuer Cytostatika wird diskutiert.
Summary
Three thousand Yoshida sarcoma cells were inoculated into the wall of the descending colon of each of 120 male Sprague-Dawley rats. On day 8 after the tumor implantation, the animals were at random divided into four groups of 30 rats each. The effect of cyclophosphamide (70 mg/kg), BCNU (25 mg/kg), and methyl-CCNU (45 mg/kg) after single i.p. application was investigated. The Yoshida sarcoma transplanted into the colon is sensitive to all three chemotherapeutic drugs. At the doses given cyclophosphamide showed the best results. The two nitrosoureas had a comparable antitumor activity but methyl-CCNU showed a more distinct toxic effect.
The introduction of this model for testing new cytostatics in animal experiments is discussed.
References
Ahmann, D.L.: Nitrosoureas in the management of disseminated malignant melanoma. Cancer Treat. Rep. 60, 747–750 (1976)
Baker, L.H., Talley, R.W., Matter, R. et al.: Phase III comparison of the treatment of advanced gastrointestinal cancer with bolus weekly 5-fluorouracil. A South West Oncology Group Study. Cancer 38, 1–7 (1976)
Baker, L.H., Vaitkevicius, V.K., Gehan, E.: Randomized prospective trial comparing 5-fluorouracil (NSC-19893) to fluorouracil and methyl CCNU (NSC-95441) in advanced gastrointestinal cancer. Cancer Treat. Rep. 60, 733–737 (1976)
Ball, C.R., Double, J.A.: Transplantable colon tumors as chemotherapy screening models. Cancer 36, 2437–2440 (1975)
Bhide, S., Habs, M., Reinbold, H., Schmähl, D.: Studies on the effect of cyclophosphamide on Yoshida sarcoma ascites cells implanted into the colon wall of rats. Arzneimittelforsch. (Drug Res.) 29, 477–479 (1979)
Carter, S.K., Shabel, F.M., Jr., Broder, J.E.: 1-3-Bis-(2-chloroethyl)-1-nitrosourea (BCNU) and other nitrosoureas in cancer treatment. Adv. Cancer Res. 16, 237–332 (1972)
Douglass, D., Levin, P.T., Moertel, C.G.: Nitrosoureas: Useful agents for the treatment of advanced gastrointestinal cancer. Cancer Treat. Rep. 60, 769–779 (1976)
Livingston, R.B.: Nitrosourea combinations in lung cancer. Cancer Treat. Rep. 60, 757–759 (1976)
Moertel, C.G.: Therapy of advanced gastrointestinal cancer with the nitrosoureas. Cancer Chem. Rep. 4, 27–34 (1973)
Moertel, C.G., Schutt, A.J., Reisemeier, R.J.: Therapy for gastrointestinal cancer with the nitrosoureas alone and in drug combination. Cancer Treat. Rep. 60, 729–732 (1976)
Moertel, C.G.: Cancer Campaign, Vol. 2, pp. 207–216. Stuttgart, New York: G. Fischer 1978
Shabel, F.M.: Nitrosoureas: A review of experimental antitumor activity. Cancer Treat. Rep. 60, 665–698 (1976)
Sych, F., Habs, M., Schmähl, D.: Chemotherapy studies in autochthonous rat tumors. Intestinal cancer. Z. Krebsforsch. 92, 105–117 (1978)
Traum, B.L., Haut, A., Rivkin, S.: A phase II study of methyl CCNU in the treatment of solid tumors and lymphomas. A South West Oncology Group Study. Cancer 35, 1148–1153 (1975)
Walker, M.D., Gehan, E.A.: Clinical studies in malignant gliomas and their treatment with nitrosoureas. Cancer Treat. Rep. 60, 713–716 (1976)
Wasserman, T.H., Slavik, M., Carter, S.K.: Clinical comparison of the nitrosoureas. Cancer 36, 1258–1262 (1975)
Wolf, J.: Nitrosoureas as single agents in the treatment of pulmonary cancer. Cancer Treat. Rep. 60, 653–656 (1976)
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Bhide, S., Habs, M., Reinbold, H. et al. Antitumor activity of 1,3-bis-(2-chloroethyl)-1-nitrosourea (BCNU) and 1-(2-chloroethyl)-3-(4-methyl-cyclohexyl)-1-nitrosourea (MeCCNU) on yoshida sarcoma ascites cells implanted into the colon wall of rats. J Cancer Res Clin Oncol 94, 63–67 (1979). https://doi.org/10.1007/BF00405350
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DOI: https://doi.org/10.1007/BF00405350
Schlüsselwörter
- Sprague-Dawley-Ratten
- Verimpfen von Yoshida-Sarkom-Zellen
- Colon descendens
- Cyclophosphamid
- BCNU
- Methyl-CCNU