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Serial determinations of “blast cell count“ during kidney allograft rejection in dogs

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Research in Experimental Medicine

Summary

Cellular and humoral responses were quantitated during acute and hyperacute kidney allograft rejection in dogs. Elevatednumbers of DNA synthesizing leukocytes appeared in the peripheral blood during primary rejection. These modifications were quantitated by serial determinations of the rate of tritiated thymidine incorporation in blood mononuclear cell suspensions. The rise of DNA synthesizing cells was paralleled by progressive mononuclear cell infiltrations in the kidney parenchyma. The decline in the level of tritiated thymidine incorporation coincided with the appearance of cytotoxic antibodies and with extensive kidney damage. Hyperacute rejections occurred in all these animals after secondary transplantation of a kidney from the original donor. All animals showed cytotoxic antibodies and displayed no increase in the rate of DNA synthesizing blood mononuclear cells.

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References

  1. Brack, G. H., Miller, J.: Changes in human mixed lymphocyte culture reactivity as an indicator of kidney rejection. Transplant Proc.4, 655 (1972)

    PubMed  Google Scholar 

  2. Buda, J., Suciu-Foca, N., Siegal, B., Jacob, J., Patner, A., Reemtsma, K.: Determination of lymphocyte activation in transplant recipients. Proceedings of the XVIII Biennial International Congress of the International College of Surgeons, Rome. Excerpta Medica (1972)

  3. Ceppellini, R., Bonnard, G. D., Coppo, F., Miggiano, V. C., Popisil, M., Curtoni, E. S., Pelligrino, M.: Mixed leukocyte cultures and HL-A antigens. 1. Reactivity of young fetuses, newborns and mothers at delivery. Transplant Proc.3, 58 (1971)

    PubMed  Google Scholar 

  4. Dimitriu, A., Debray-Sachs, M., Descamps, B., Sultan, C., Hamburger, J.: Tritiated thymidine incorporation in blood leukocytes of renal allograft recipients. Transplant Proc.3, 1577 (1971)

    Google Scholar 

  5. Hamburger, J., Dimitriu, A., Banker, L., Debray-Sachs, M., Auvert, J.: Collection of lymph from kidneys homotransplanted in man: cell transformationin vivo. Nature232, 633 (1971)

    PubMed  Google Scholar 

  6. Harris, J., Bagai, R., Rashid, A., Hyslop, D., Stewart, T.: Nucleic acid synthesis in peripheral blood lymphocytes as an indicator of rejection. Transplant Proc.4, 659 (1972)

    PubMed  Google Scholar 

  7. Häyry, P., Lindstrom, B. L., Virolainen, M., Pasternack, A., Lindfors, O.: Immunobiological diagnosis of rejection in dogs with renal allografts. Surgery71, 494 (1972)

    PubMed  Google Scholar 

  8. Häyry, P., Pasternack, A., Virolainen, M.: Cell proliferation within graft and in blood during renal allograft rejection. Transplant Proc.4, 195 (1972)

    PubMed  Google Scholar 

  9. Hersh, E. M., Butler, W. T., Rosen, R. D., Morgan, R. O., Suki, W.:In vitro studies of the human response to organ allografts: appearance and detection of circulating activation lymphocytes. J. Immunol.107, 571 (1971)

    PubMed  Google Scholar 

  10. Merrill, J. P.: Diagnosis and management of rejection in allograft kidneys. Transplant Proc.3, 287 (1971)

    PubMed  Google Scholar 

  11. Miller, J., Hattler, B., Davis, M., Johnson, M. C.: Cellular and humoral factors governing caine mixed lymphocyte cultures after renal transplantation. Transplantation12, 65 (1971)

    PubMed  Google Scholar 

  12. Parker, J. R., Mowbray, J. F.: Peripheral blood leucokyte changes during human renal allograft rejection. Transplantation11, 201 (1971)

    PubMed  Google Scholar 

  13. Pederson, N. C., Morris, B.: The role of the lymphatic system in the rejection of homografts: a study of lymph from renal transplants. J. exp. Med.131, 936 (1970)

    PubMed  Google Scholar 

  14. Reemtsma, K.: Heterotransplantation: theoretical considerations. Transplant. Proc.3, 49 (1971)

    PubMed  Google Scholar 

  15. Suciu-Foca, N., Buda, J., Thiem, T., Suciu, T.: Serial determinations of blast cell count during allograft rejection in rats. Transplantation14, 711 (1972)

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Surgery, Francis Delafield Hospital, College of Physicians and Surgeons, of Columbia Presbyterian Medical Center, 10032, New York, New York, USA

    J. Buda (Director of Surgery), N. Suciu-Foca, T. Thiem & K. Reemtsma

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  1. J. Buda
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  2. N. Suciu-Foca
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  3. T. Thiem
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  4. K. Reemtsma
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Additional information

Supported by Grant 0113-26920, N.Y.S.C. 48408 from the Kidney Disease Institute, State of New York.

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Buda, J., Suciu-Foca, N., Thiem, T. et al. Serial determinations of “blast cell count“ during kidney allograft rejection in dogs. Res. Exp. Med. 162, 227–233 (1974). https://doi.org/10.1007/BF01851244

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  • DOI: https://doi.org/10.1007/BF01851244

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