Summary
The immediate effect of CO2-laser incision to the dorsal skin and underlying muscular tissue of rats was studied by light and electron microscopical methods. In the subcutaneous layer of skeletal muscle cells an increased susceptibility was found, resulting in distinct zones of cellular changes which are attributed mainly to thermal effects of the laser beam. The importance of these findings for the surgical application of lasers is discussed.
Zusammenfassung
Der Zustand der dorsalen Haut und des darunterliegenden Muskelgewebes der Ratte wurde unmittelbar nach CO2-Laser-Schnitten mit Hilfe von licht- und elektronenmikroskopischen Methoden untersucht. In der subkutanen Skelettmuskelschicht konnte eine erhöhte Empfindlichkeit des Gewebes festgestellt werden, die in einer ausgeprägten Abfolge von Zellveränderungen ihren Ausdruck findet. Diese Schädigungen werden hauptsächlich den thermischen Effekten der Laserstrahlung zugeschrieben. Die Bedeutung dieser Untersuchungsergebnisse in Hinblick auf die Verwendung von Laser in der Chirurgie wird besprochen.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Amy, R. L., Storb, R.: Selective mitochondrial damage by a ruby laser microbeam using janus green as a vital specific dye absorbent. Science150, 756–758 (1965)
Ben-Bassat, Mi., Ben-Bassat, Mo., Kaplan, I.: A study of the ultrastructural features of the cut margin of skin and mucous membrane specimens excised by carbon dioxide laser. J. Surg. Res.21, 77–84 (1976)
Derr, V. W., Klein, E., Fine, S.: Free radical occurrence in some laser-irradiated biological materials. Fed. Proc.24, Suppl. 14, S99-S103 (1965)
Dittrich, H., Drüen, B., Schlake, W.: Die Anwendung des Lasers in der Thorax-, Herz- und Gefäßchirurgie. Thoraxchir.23, 505–510 (1975)
Dittrich, H., Lunkenheimer, P. P., Drüen, B., Mey, J.: Experimentelle Ergebnisse zur Laserchirurgie. 119. Tag. der Vereinigung Nordwestdeutscher Chirurgen, Lübeck, 2–4. Juni 1977
Eichler, J., Knof, J., Lenz, H., Salk, J., Schäfer, G.: Temperature distribution in tissue during laser irradiation. Rad. Environm. Biophys.15, 277–287 (1978)
Frishman, A., Gassner, S., Kaplan, I., Ger, R.: Excision of subcutaneous fibrosarcoma in mice. Israel. J. Med. Sci.10, 637–641 (1974)
Gonzales, R., Edlich, R. F., Goodale, R. L., Polanyi, T. G., Borner, B. A., Wangensteen, O. H.: Rapid control of hepatic haemorrhage by laser radiation. Surg. Gynecol. Obstet.121, 198–200 (1970)
Gorisch, W., Boergen, K. P., McCord, R. C., Weinberg, W., Hillenkamp, F.: Temperature measurements of isolated mesenteric blood vessels of the rabbit during laser irradiation. In: Laser Surgery II, Kaplan, I. (ed.), pp. 202–207. Jerusalem: Academic Press 1978
Hall, R. R.: Haemostatic incision of the liver: Carbondioxide laser compared with surgical diathermy. Br. J. Surg.58, 538–540 (1971)
Jako, G. J.: Laser surgery of the vocal cords. Laryngoscope82, 2204–2215 (1972)
Kaplan, I., Ger, R.: The carbon dioxide laser in clinical surgery. Israel. J. Med. Sci.9, 79–83 (1973)
Luft, J. H.: Improvements in epoxy resin embedding methods. J. Biophys. Biochem. Cyt.9, 409–414 (1961)
Lunkenheimer, P. P., Drüen, B., Sowislo, W., Freytag, G., Weritz, D., Dittrich, H.: Wound healing after scalpel-, laser- and thermocautery-surgery. In: Laser Surgery II, Kaplan, I. (ed.), pp. 334–349. Jerusalem: Academic Press 1978
Lynn, J. A.: Rapid toluidine blue staining of epon-embedded and mounted “adjacent” sections. Am. J. Clin. Pathol.44, 57–58 (1965)
Mendelson, J. A., Ackerman, N. B.: Studies of biologically significant forces following laser irradiation. Fed. Proc.24, Suppl. 14, 111–115 (1965)
Mihashi, S., Jako, G. J., Incze, J., Strong, M. S., Vaughan, C. W.: Laser surgery in otolaryngology: Interaction of CO2 laser and soft tissue. Ann. N.Y. Acad. Sci.267, 263–294 (1976)
Mullins, F., Jennings, B., McClusky, L.: Liver resection with continuous wave carbon dioxide laser. Am. Surg.34, 717–722 (1968)
Palade, G. E.: A study of fixation for electron microscopy. J. Exp. Med.95, 285–298 (1952)
Pariente, R.: The Sharplan 791 laser in the treatment of skin tumors. In: Laser Surgery, Kaplan, I. (ed.), pp. 31–53. Jerusalem: Academic Press 1976
Plenk, H., Kyrle, P., Fischer, R.: Bringt der CO2-Laser-Schnitt Vorteile? Morphologische und experimentelle Untersuchungen. Wien. Klin. Wochenschr. (in press, 1979)
Reynolds, E. S.: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell. Biol.17, 208–212 (1963)
Rounds, D. E., Olson, R. S., Johnson, F. M.: The effect of laser on cellular respiration. Z. Zellforsch.87, 193–198 (1968)
Strahs, K. R., Burt, J. M., Berns, M. W.: Contractility changes in cultured cardiac cells following laser microirradiation of myofibrils and the cell surface. Exp. Cell Res.113, 75–83 (1978)
Author information
Authors and Affiliations
Additional information
This study was supported by a grant of the “Dr. Theodor Körner-Foundation”
Prof. Dr. P. Kyrle died before the paper was completed
Rights and permissions
About this article
Cite this article
Viehberger, G., Fischer, R., Kyrle, P. et al. Ultrastructure of skeletal muscle after CO2-laser incision. Res. Exp. Med. 176, 69–79 (1979). https://doi.org/10.1007/BF01852112
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01852112