Summary
31P-NMR spectra were obtained from adenosine-5′-tetraphosphate under a variety of conditions and the four discernible resonances assigned. The pK-value of the metal-free compound was determined to be 6.4, the pK-value of the Mg2+ complex to be 5.3. The dissociation constant for the AP4⋅Mg2+ complex was estimated to be 10−4 M from the downfield shift of the resonances assigned to theγ- andδ-phosphorus nuclei. The binding of a second metal ion can also be followed by NMR; the dissociation constant for this ternary complex is several orders of magnitude larger than that for the binary complex.
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Klaus, W., Rösch, P. & Goody, R.S. 31P-NMR spectra of AP4 . Res. Exp. Med. 185, 145–150 (1985). https://doi.org/10.1007/BF01854900
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DOI: https://doi.org/10.1007/BF01854900