Abstract
We produced monoclonal antibodies against the adult antigen ofParagonimus westermani to investigate the expression of the stage-specific antigen of adult flukes. Two hybridoma cell lines, A1-2 and A4-1, were established. A1-2 reacted specifically only with the adult antigen in an enzyme-linked immunosorbent assay (ELISA), whereas A4-1 reacted with both adult and larval antigens. By immunoblotting analysis, A1-2 was found to react with four bands with molecular weights of 35000, 17000, 15500, and 12500 in the adult antigen but with none in the larval antigen. A4-1 was found to bind to the band of 27000 daltons in the adult antigen as well as those of 28000 and 26000 daltons in the larval antigen. By immunohistochemical methods, a positive reaction was observed only in the parenchymal tissues of the adult flukes with A1-2. A 4-1 reacted with the substance that located on the gut epithelium and in the luminal contents of both the adult and larval flukes. These results indicate that the adultP. westermani possesses an antigen or antigenic determinant specific to the adult stage, as well as that common to both the larval and adult stages.
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Araki K, Nogami S, Nakamura M, Akashi M (1985) Many cases of human paragonimiasis westermani in Oita Prefecture. Jpn J Parasitol 34 (Suppl):73
DeLellis RA (1981) Basic techniques of immunohistochemistry. In: DeLellis RA (ed) Diagnostic immunohistochemistry. Masson, New York, pp 7–16
Kanzaki T, Tanaka M, Nozaki T, Kanazawa T, Kobayashi M, Yokogawa M, Yoshida S (1985) Early clinical symptoms and signs of paragonimiasis westermani, time course when intradermal test withParagonimus westermani antigen turns positive and effects of praziquantel to paragonimiasis. Jpn J Thoracic Dis 23:368–374
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
Miyazaki I (1982) Paragonimiasis. In: Hillyer GV, Hopla CE (eds) CRC handbook series in zoonoses, section C. Parasitic zoonoses, vol 3. CRC Press Inc., Boca Raton, pp 143–164
Norden AP, Aronstein WS, Strand M (1982)Schistosoma mansoni: identification, characterization, and purification of the spine glycoprotein by monoclonal antibody. Exp Parasitol 54:432–442
Strand M, McMillan AX, Pan XQ (1982)Schistosoma mansoni: reactivity with infected human sera and monoclonal antibody characterization of a glycoprotein in different developmental stages. Exp Parasitol 54:145–156
Sugiyama H, Hinoue H, Akasaka K, Sugimoto M, Kozaki S, Horiuchi T, Tomimura T (1987a) Enzyme-linked immunosorbent assay for the detection of serum antibodies in cats and rats infected withParagonimus westermani. Jpn J Parasitol 36 (Suppl):32
Sugiyama H, Sugimoto M, Akasaka K, Horiuchi T, Tomimura T, Kozaki S (1987b) Characterization and localization ofParagonimus westermani antigen stimulating antibody formation in both infected cat and rat. J Parasitol 73:363–367
Suzuki T, Tani S, Ishida K, Ohmori Y, Sugawara M, Banzai A (1978) A case of subcutaneous infection with lung fluke,Paragonimus westermani, in Akita Prefecture, Japan. Jpn J Parasitol 27:91–96
Towbin H, Staehelin TJ, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76:4350–4354
Yokogawa M (1965) Paragonimus and paragonimiasis. In: Daves B (ed) Advances in parasitology, vol 3. Academic Press, London, pp 99–158
Yokogawa M (1982) Paragonimiasis. In: Hillyer GV, Hopla CE (eds) CRC handbook series in zoonoses, section C. Parasitic zoonoses, vol 3. CRC Press Inc., Boca Raton, pp 143–164
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Sugiyama, H., Hinoue, H., Katahira, J. et al. Production of monoclonal antibody to characterize the antigen ofParagonimus westermani . Parasitol Res 75, 144–147 (1988). https://doi.org/10.1007/BF00932714
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DOI: https://doi.org/10.1007/BF00932714