Skip to main content
SpringerLink
Log in

Synthesis of mitochondrial DNA during the cell cycle of the petite negative yeast Schizosaccharomyces pombe

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

Synthesis of mitochondrial DNA (mitDNA) and nuclear DNA (nucDNA) during growth of synchronously dividing cultures of Schizosaccharomyces pombe (S. pombe) was followed by pulse labelling with radioactive adenine and determination of its rate of incorporation into total protoplast DNA and into the DNA of DNase-treated mitochondria at different stages of the cell cycle. It could be demonstrated that both mitDNA and nucDNA were synthesised discontinuously and at different points in the cell cycle.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bostock CJ (1969) Mitochondrial DNA in the fission yeast Schizosaccharomyces pombe. Biochim Biophys Acta 195:579–581

    Google Scholar 

  • Bostock CJ, Donachie WD, Masters M, Mitchison JM (1966) Synthesis of enzymes and DNA in synchronous cultures of Schizosaccharomyces pombe. Nature 21:808–810

    Google Scholar 

  • Blamire J, Cryer DR, Finkelstein DB, Marmur J (1972) Sedimentation properties of yeast nuclear and mitochondrial DNA. J Mol Biol 67:11–24

    Google Scholar 

  • Cottrell SF, Avers CJ (1970) Evidence of mitochondrial synchrony in synchronous cultures of yeast. Biochem Biophys Res Commun 38:973–980

    Google Scholar 

  • Del Giudice L, Wolf K (1980) Evidence for a joint control of nuclear and mitochondrial DNA synthesis in the petite negative yeast Schizosaccharomyces pombe. In: Schwemmler W and Schenk HEA (eds) Endocytobiology. Walter de Gruyter, Berlin-New York, pp 779

    Google Scholar 

  • Del Giudice L, Wolf K, Buono C, Manna F (1981) Nucleo-cytoplasmic interactions in the petite negative yeast Schizosaccharomyces pombe. Inhibition of nuclear and mitochondrial DNA syntheses in the absence of cytoplasmic protein synthesis. Mol Gen Genet 181:306–308

    Google Scholar 

  • Del Giudice L, Wolf K, Sassone-Corsi P, Alvino C (1978) Circular molecules of heterogeneous size from mitochondrial fractions of the petite negative yeast Schizosaccharomyces pombe. Mol Gen Genet 164:289–293

    Google Scholar 

  • Sena PE, Welch JW, Halvorson HO, Fogel S (1975) Nuclear and mitochondrial deoxyribonucleic acid replication during mitosis in Saccharomyces cerevisiae. J Bacteriol 123:497–504

    Google Scholar 

  • Smith D, Tauro P, Schweizer E, Halvorson HO (1968) The replication of mitochondrial DNA during the cell cycle in Saccharomyces lactis. Proc Natl Acad Sci USA 60:936–942

    Google Scholar 

  • Tabak HF, Weijers PJ (1976) The genetic complexity of Schizosaccharomyces pombe mitochondrial DNA. FEBS Letters 69:211–215

    Google Scholar 

  • Wells JR (1974) Mitochondrial DNA synthesis during the cell cycle of Saccharomyces cerevisiae. Exptl Cell Res 85:278–286

    Google Scholar 

  • Williamson DH, Moustacchi E (1971) The synthesis of mitochondrial DNA in synchronously dividing cultures of Saccharomyces cerevisiae. Biochem Biophys Res Commun 42:195–201

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. C. N. R., International Institute of Genetics and Biophysics, Naples, Italy

    L. Del Giudice, F. Manna & N. Pagliuca

  2. Genetisches Institut der Universität München, Munich, Federal Republic of Germany

    K. Wolf

Authors
  1. L. Del Giudice
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. K. Wolf
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. F. Manna
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. N. Pagliuca
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by W. Gajewski

Rights and permissions

Reprints and permissions

About this article

Cite this article

Del Giudice, L., Wolf, K., Manna, F. et al. Synthesis of mitochondrial DNA during the cell cycle of the petite negative yeast Schizosaccharomyces pombe . Molec. Gen. Genet. 182, 252–254 (1981). https://doi.org/10.1007/BF00269666

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00269666

Keywords

Search

Navigation