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Tzs, a nopaline Ti plasmid gene from Agrobacterium tumefaciens associated with trans-zeatin biosynthesis

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Summary

The tzs gene, present in nopaline Ti plasmids, confers on Agrobacterium tumefaciens the ability to produce the phytohormone, trans-zeatin (Regier and Morris (1982) Biochem Biophys Res Comm 104:1560–1566). This gene has now been cloned from the nopaline Ti plasmid pTiC58. It occurs outside the T-DNA in a region close to that associated with virulence functions. Sequence studies indicate that tzs has substantial homology with the T-region gene, ipt, which is known to encode a dimethylallylpyrophosphate transferase, the first enzyme of the cytokinin biosynthetic pathway. As expected from its homology with ipt, tzs possesses significant DMA transferase activity but when expressed in Escherichia coli it causes secretion of trans-zeatin.

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References

  • Akiyoshi DE, Morris RO, Hinz R, Mischke BS, Kosuge T, Garfinkel DJ, Gordon MP, Nester EW (1983) Cytokinin-auxin balance in crown gall tumors is regulated by specific loci in the T-DNA. Proc Natl Acad Sci USA 80:407–411

    Google Scholar 

  • Akiyoshi DE, Regier DA, Gordon MP (1985) Cloning and nucleotide sequence of the tzs gene from Agrobacterium tumefaciens strain T3. Nucleic Acids Res 13:2773–2788

    Google Scholar 

  • Barker RF, Idler KB, Thompson DV, Kemp JD (1983) Nucleotide sequence of the T-DNA from Agrobacterium tumefaciens octopine Ti plasmid pTi15955. Plant Mol Biol 2:335–350

    Google Scholar 

  • Barry GF, Rogers SG, Fraley RT, Brand L (1984) Identification of a cloned cytokinin biosynthetic gene. Proc Natl Acad Sci USA 81:4776–4780

    Google Scholar 

  • Biggin MD, Gibson TJ, Hong GF (1983) Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination. Proc Natl Acad Sci USA 80:3963–3965

    Google Scholar 

  • Braun AC (1958) A physiological basis for the autonomous growth of the crown gall tumor cell. Proc Natl Acad Sci USA 44:344–349

    Google Scholar 

  • Buchmann L, Marner F-J, Schröder G, waffenschmidt S, Schröder J (1985) Tumor genes in plants: T-DNA encoded cytokinin biosynthesis. EMBO J 4:853–859

    Google Scholar 

  • Calhoun DH, Gray JE (1981) Detection of proteins coded by cloned DNA segments. BRL Focus 3:10–11

    Google Scholar 

  • Chen CM, Melitz DK (1979) Cytokinin biosynthesis in a cell-free system from cytokinin-autotrophic tobacco tissue cultures. FEBS Lett 107:15–20

    Google Scholar 

  • Chilton M-D, Currier TC, Farrand SK, Bendich AJ, Gordon MP, Nester EW (1974) Agrobacterium tumefaciens DNA and PS8 bacteriophage DNA not detected in crown gall tumors. Proc Natl Acad Sci USA 71:3672–3676

    Google Scholar 

  • Chilton M-D, Drummond MH, Merlo DJ, Sciaky D, Montoya AL, Gordon MP, Nester EW (1977) Stable incorporation of plasmid DNA into higher plant cells: the molecular basis of crown gall tumorigenesis. Cell 11:263–271

    Google Scholar 

  • Depicker A, DeWilde M, DeVos G, DeVos R, Van Montagu M, Schell J (1980) Molecular cloning of overlapping segments of the nonpaline Ti-plasmid pTiC58 as a means of restriction endonuclease mapping. Plasmid 3:193–211

    Google Scholar 

  • Ditta G, Stanfield S, Corbin D, Helinski DR (1980) Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti. Proc Natl Acad Sci USA 77:7347–7351

    Google Scholar 

  • Einset JW (1980) Cytokinins in tobacco crown gall tumors. Biochem Biophys Res Commun 93:510–515

    Google Scholar 

  • Engler G, Depicker A, Maenhaut R, Villarroel R, Van Montagu M, Schell J (1981) Physical mapping of DNA base sequence homologies between an octopine and a nopaline Ti plasmid of Agrobacterium tumefaciens. J Mol Biol 152:183–208

    Google Scholar 

  • Garfinkel DJ, Simpson RB, Ream LW, White FF, Gordon MP, Nester EW (1981) Genetic analysis of crown gall: Fine structure map of the T-DNA by site-directed mutagenesis. Cell 27:143–153

    Google Scholar 

  • Greshoff PM, Skotnicki ML, Rolfe BG (1979) Crown gall teratoma formation is plasmid and plant controlled. J Bacteriol 137:1020–1021

    Google Scholar 

  • Hagiya M, Close TJ, Tait RC, Kado CI (1985) Identification of pTiC58 plasmid-encoded proteins for virulence in Agrobacterium tumefaciens. Proc Natl Acad Sci USA 82:2669–2673

    Google Scholar 

  • Hamilton RH, Fall MZ (1971) The loss of tumor-initiating ability in Agrobacterium tumefaciens by incubation at high temperature. Experientia 27:229–230

    Google Scholar 

  • Hoekema A, de Pater BS, Fellinger AJ, Hooykaas PJJ, Schilperoort RA (1984) The limited host range of an Agrobacterium tumefaciens strain extended by a cytokinin gene from a wide host range T-region. EMBO J 3:3043–3047

    Google Scholar 

  • Holsters M, Silva B, Van Vliet F, Genetello C, DeBlock M, Dhaese P, Depicker A, Inze D, Engler G, Villarroll R, Van Montagu M, Schell J (1980) The functional organization of the A. tumefaciens plasmid pTiC58. Plasmid 3:212–230

    Google Scholar 

  • Hommes NG, Akiyoshi DE, Morris RO (1985) Assay and partial purification of the cytokinin biosynthetic enzyme dimethylallylpyrophosphate: 5′AMP transferase. Methods Enzymol 110:340–347

    Google Scholar 

  • Knauf VC, Nester EW (1982) Wide host range cloning vectors: a cosmid clone bank of an Agrobacterium Ti plasmid. Plasmid 8:45–54

    Google Scholar 

  • Letham DS, Tao GQ, Parker CW (1982) An overview of cytokinin metabolism. In: Wareing PF (ed) Plant growth substances. Academic Press, London, pp 143–153

    Google Scholar 

  • Lipman DJ, Pearson WR (1985) Rapid and sensitive protein similarity searches. Science 227:1435–1441

    Google Scholar 

  • Lundquist RC, Close TJ, Kado CI (1984) Genetic complementation of Agrobacterium tumefaciens Ti plasmid mutants in the virulence region. Mol Gen Genet 193:1–7

    Google Scholar 

  • MacDonald EMS, Akiyoshi DE, Morris RO (1982) Combined high performance liquid chromatography-radioimmunoassay for cytokinins. J Chromatogr 214:101–109

    Google Scholar 

  • MacDonald EMS, Morris RO (1985) Isolation of cytokinins by immunoaffinity chromatography and analysis by HPLC-radioimmunoassay. Methods Enzymol 110:347–358

    Google Scholar 

  • Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, New York

    Google Scholar 

  • Miller CO (1974) Ribosyl-trans-zeatin, a major cytokinin produced by crown gall tumor tissue. Proc Natl Acad Sci USA 71:334–338

    Google Scholar 

  • Miller J (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory, New York

    Google Scholar 

  • Morris RO, Akiyoshi DE, MacDonald EMS, Morris JW, Regier DA, Zaerr JB (1982) Cytokinin metabolism in relation to tumor induction by Agrobacterium tumefaciens. In: Wareing PF (ed) Plant growth substances. Academic Press, London, pp 175–183

    Google Scholar 

  • Norrander J, Kempe T, Messing J (1983) Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis. Gene 26:101–106

    Google Scholar 

  • Regier DA, Morris RO (1982) Secretion of trans-zeatin by Agrobacterium tumefaciens: a function determined by the nopaline Ti plasmid. Biochem Biophys Res Commun 104:1560–1566

    Google Scholar 

  • Roberts TM, Bikel I, Yocum RR, Livingston DM, Ptashne M (1979) Synthesis of Simian virus 40 T-antigen in Escherichia coli. Proc Natl Acad Sci USA 76:5596–5600

    Google Scholar 

  • Rosenberg M, Court D (1979) Regulatory sequences involved in the promotion and termination of RNA transcription. Annu Rev Genet 13:319–353

    Google Scholar 

  • Sancar A, Hack AM, Rupp WD (1979) Simple method for identification of plasmid-coded proteins. J Bacteriol 137:692–693

    Google Scholar 

  • Sanger F, Nicklen S, Coulsen AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467

    Google Scholar 

  • Scott IM, Browning G, Eagles J (1980) Ribosylzeatin and zeatin in tobacco crown gall tissues. Planta 147:269–273

    Google Scholar 

  • Shine J, Dalgarno L (1974) The 3′-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites. Proc Natl Acad Sci USA 71:1342–1346

    Google Scholar 

  • Vieira J, Messing J (1982) The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19:259–268

    Google Scholar 

  • Watson B, Currier TC, Gordon MP, Chilton M-D, Nester EW (1975) Plasmid required for virulence of Agrobacterium tumefaciens. J Bacteriol 123:255–264

    Google Scholar 

  • Wilbur WJ, Lipman DJ (1983) Rapid similarity searches of nucleic acid and protein data banks. Proc Natl Acad Sci USA 80:726–730

    Google Scholar 

  • Zaenen I, Van Larebeke N, Teuchy H, Schell J (1974) Supercoiled circular DNA in crown gall inducing Agrobacterium strains. J Mol Biol 86:109–127

    Google Scholar 

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Authors and Affiliations

  1. Department of Agricultural Chemistry, Oregon State University, 97331, Corvallis, OR, USA

    J. S. Beaty, G. K. Powell, L. Lica, D. A. Regier, E. M. S. MacDonald, N. G. Hommes & R. O. Morris

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  1. J. S. Beaty
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  2. G. K. Powell
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  3. L. Lica
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  4. D. A. Regier
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  5. E. M. S. MacDonald
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  6. N. G. Hommes
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  7. R. O. Morris
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Communicated by J. Schell

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Beaty, J.S., Powell, G.K., Lica, L. et al. Tzs, a nopaline Ti plasmid gene from Agrobacterium tumefaciens associated with trans-zeatin biosynthesis. Molec Gen Genet 203, 274–280 (1986). https://doi.org/10.1007/BF00333966

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  • DOI: https://doi.org/10.1007/BF00333966

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