Summary
A general in vivo procedure for cloning Escherichia coli genes into cosmids has been developed. The method we describe here uses a deleted Mu phage (a mini-Mu) to transpose E. coli genes into cosmids during mini-Mu replication. The resulting cosmids clones are packaged in-vivo into λ phage particles. Plasmids carrying a particular DNA sequence can be selectively recovered after infection of a new host with the in vivo constructed genomic cosmid library. This system was used succesfully to clone several E. coli genes.
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Communicated by J. Schell
Dedicated to Dr. Luis F. Leloir on the occasion of his 80th birthday, September 6, 1986
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de Mendoza, D., Gramajo, H.C. & Rosa, A.L. In vivo cloning of DNA into multicopy cosmids by mini-Mu-cosduction. Mol Gen Genet 205, 546–549 (1986). https://doi.org/10.1007/BF00338096
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DOI: https://doi.org/10.1007/BF00338096