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The cytoplasmic ribosomes of Chlamydomonas reinhardtii: Characterization of antibiotic sensitivity and cycloheximide-resistant mutants

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Summary

In vitro protein synthesis was used to characterize the antibiotic sensitivity of cytoplasmic ribosomes from wild-type and antibiotic-resistant strains of Chlamydomonas reinhardtii. Cytoplasmic ribosomes from two cycloheximide-resistant mutants, act-1 and act-2, were resistant to the antibiotic in vitro. The alteration effected by the act-1 mutation, which was dominant in diploids, was localized to the large subunit of the cytoplasmic ribosomes, but no ribosomal protein alterations were detected using two-dimensional gel electrophoresis. The act-2 mutation, which was semidominant in diploids, was frequently associated with a charge alteration in the large subunit ribosomal protein (r-protein) cyL38 that segregated independently from the antibiotic-resistant phenotype in crosses.

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Author notes

  1. G. H. Fleming

    Present address: Department of Biochemistry, University of North Carolina, FLOB 2312-H, 27514, Chapel Hill, NC, USA

Authors and Affiliations

  1. Department of Botany, Duke University, 27706, Durham, NC, USA

    G. H. Fleming & J. E. Boynton

  2. Department of Zoology, Duke University, 27706, Durham, NC, USA

    N. W. Gillham

Authors
  1. G. H. Fleming
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  2. J. E. Boynton
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  3. N. W. Gillham
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Communicated by H. Böhme

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Fleming, G.H., Boynton, J.E. & Gillham, N.W. The cytoplasmic ribosomes of Chlamydomonas reinhardtii: Characterization of antibiotic sensitivity and cycloheximide-resistant mutants. Mol Gen Genet 210, 419–428 (1987). https://doi.org/10.1007/BF00327192

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  • DOI: https://doi.org/10.1007/BF00327192

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