Summary
Repair of methylated bases in Saccharomyces cerevisiae was measured by two methods: in vitro in cell extracts, and in vivo, by determining the loss of methylated bases from yeast DNA after treatment of stationary cultures with [3H]-N-methyl-N′-nitro-N-nitrosoguanidine. Whereas no repair activity could be detected by the in vitro method, the methylated bases were removed in vivo very efficiently. These contradictory results of in vitro and in vivo repair measurements suggest that either the repair enzymes of yeast are sufficiently different from those of bacteria and mammalian cells that they are not active in the in vitro assay, or that methylated bases are repaired in yeast by a different pathway.
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Communicated by B. Kilbey
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Goth-Goldstein, R., Johnson, P.L. Repair of alkylation damage in Saccharomyces cerevisiae . Mol Gen Genet 221, 353–357 (1990). https://doi.org/10.1007/BF00259399
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DOI: https://doi.org/10.1007/BF00259399