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Escherichia coli mutY-dependent mismatch repair involves DNA polymerase I and a short repair tract

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Abstract

Repair of heteroduplex DNA containing an A/G mismatch in a mutL background requires the Escherichia coli mutY gene function. The mutY-dependent in vitro repair of A/G mismatches is accompanied by repair DNA synthesis on the DNA strand bearing mispaired adenines. The size of the mufY-dependent repair tract was measured by the specific incorporation of α-[32P]dCTP into different restriction fragments of the repaired DNA. The repair tract is shorter than 12 nucleotides and longer than 5 nucleotides and is localized to the 3′ side of the mismatched adenine. This repair synthesis is carried out by DNA polymerase I.

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  1. Jyy-Jih Tsai-Wu

    Present address: University of Maryland Cancer Center, 21201, Baltimore, Maryland, USA

Authors and Affiliations

  1. Department of Biological Chemistry, School of Medicine, University of Maryland at Baltimore, 21201, Baltimore, Maryland, USA

    Jyy-Jih Tsai-Wu & A-Lien Lu

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  1. Jyy-Jih Tsai-Wu
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  2. A-Lien Lu
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Communicated by R. Devoret

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Tsai-Wu, JJ., Lu, AL. Escherichia coli mutY-dependent mismatch repair involves DNA polymerase I and a short repair tract. Molec. Gen. Genet. 244, 444–450 (1994). https://doi.org/10.1007/BF00286698

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  • DOI: https://doi.org/10.1007/BF00286698

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