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The genetics of the repair of 5-azacytidine-mediated DNA damage in the fission yeastSchizosaccharomyces pombe

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Abstract

We have recently demonstrated thatSchizosaccharomyces pombe cells treated with the nucleoside analogue 5-azacytidine (5-azaC) require previously characterised G2 checkpoint mechanisms for survival. Here we present a survey of known DNA repair mutations which defines those genes required for survival in the presence of 5-azaC. Using a combination of single-mutant and epistasis analyses we find that the excision, mismatch and recombinational repair pathways are all required in some degree for the repair of 5-azaC-mediated DNA damage. There are distinct differences in the epistatic interactions of several of the repair mutations with respect to 5-azaC-mediated DNA damage relative to UV-mediated DNA damage.

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Author notes

  1. E. M. Taylor

    Present address: MRC Cell Mutation Unit, Sussex University, BN1 9RR, Brighton, UK

Authors and Affiliations

  1. Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, P. O. Box 594, S10 2UH, Sheffield, U. K.

    V. Hegde, R. J. McFarlane, E. M. Taylor & C. Price

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  1. V. Hegde
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  2. R. J. McFarlane
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  3. E. M. Taylor
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Communicated by B. J. Kilbey

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Hegde, V., McFarlane, R.J., Taylor, E.M. et al. The genetics of the repair of 5-azacytidine-mediated DNA damage in the fission yeastSchizosaccharomyces pombe . Molec. Gen. Genet. 251, 483–492 (1996). https://doi.org/10.1007/BF02172377

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  • DOI: https://doi.org/10.1007/BF02172377

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