Summary
Hybridization of albumin clones cDNA with human DNAs digested by several restriction endonucleases reveals two Hae III polymorphisms. The first polymorphism, H1, is of low frequency (f 1=0.05); the second, which is validated by family analysis, occurs frequently (f 2=0.21) and is an intronic polymorphism, probably of substitution—base type.
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References
Barker D, White R (1982) More base-pair change polymorphisms at sites containing CpG. Cytogenet Cell Genet 32:253
Bastié-Sigeac F, Lucotte G (1983) Optimal use of restriction enzymes in the analysis of human DNA polymorphism. Hum Genet 63: 162–165
Dugaiczyk A, Law SW, Dennison OE (1982) Nucleotide sequence and the encoded amino acids of human serum albumin mRNA. Proc Natl Acad Sci USA 79:71–75
Hawkins JW, Dugaiczyk A (1982) The human serum albumin gene: structure of a unique locus. Gene 19:55–58
Kao FT, Hawkins JW, Law ML, Dugaiczyk A (1982) Assignment of the structural gene coding for albumin to human chromosome 4. Hum Genet 62:337–341
Lawn RM, Adelman J, Bock SC, Franke AE, Houck CM, Najarian RC, Seeburg PH, Wion KL (1981) The sequence of human serum albumin cDNA and its expression in E. Coli. Nucl Acids Res 9:6103–6114
Lucotte G Gal A, Nahon JL, Sala-Trepat JM (1982) Eco RI restriction site polymorphism of the albumin gene in different inbred strains of rat. Biochem Genet 20:1105–1115
Murray JC, Demopulos CM, Lawn RM, Motulsky AG (1983) Molecular genetics of human serum albumin restriction enzyme fragment length polymorphism and analbuminemia. Proc Natl Acad Sci USA 80:5951–5955
Rahuel C, Dandieu S, Chaussé AM, Mornet E, Rouger P, Cartron JP, Salmon C, Lucotte G (1983) Rev Franc Transf Immuno-hématol 26:539–552
Rigby PW, Dieckmann M, Rhodes M, Berg P (1977) Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol 113:237–249
Schäffer M, White R (1982) Three random loci in the human genome with base pair change polymorphism. Cytogenet Cell Genet 32: 314–315
Skolnick MH, Francke U (1982) Report of the committee on human gene mapping by recombinant DNA techniques. Cytogenet Cell Genet 32:194–204
Southern E (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–519
Wahl MW, Stern M, Stark (1979) Efficient transfer of large DNA fragments from agarose to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci USA 76:3683–3688
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de Souza, S.L., Frain, M., Mornet, E. et al. Polymorphisms of human albumin gene after DNA restriction by Hae III endonuclease. Hum Genet 67, 48–51 (1984). https://doi.org/10.1007/BF00270557
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DOI: https://doi.org/10.1007/BF00270557