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Regional localization and characterization of a DNA segment on the long arm of chromosome 21

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Summary

A human genomic DNA fragment, pAM37 (HGM8; D21S22), was mapped to chromosome 21q2.1-q2.21 by in situ hybridization. This segment is therefore situated on the boundary of the “pathological region” of Down syndrome. A genomic restriction map encompassing 35 kb of chromosome 21 was derived and two restriction fragment length polymorphisms (RFLPs) were mapped and characterized. A homologous sequence was detected in the mouse genome but no homologous RNA was detected in a range of human tissues. This DNA segment will contribute to the linkage mapping of chromosome 21 and will facilitate delineation of the pathological region of Down syndrome.

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Author notes

  1. G. S. Rajendran

    Present address: Institute of Molecular and Cellular Biology, University of Singapore, Singapore

Authors and Affiliations

  1. Neurochemistry Department, Institute of Neurology, University of London, Queen Square, WC1N 3BG, London, UK

    D. N. Cooper, A. M. Goate, G. S. Rajendran & L. Lim

  2. Institut für Humangenetik der Universität, Gosslerstrasse 12d, D-3400, Göttingen, Federal Republic of Germany

    S. C. Niemann & J. Schmidtke

  3. MRC Clinical and Population Cytogenetics Unit, Western General Hospital, Crewe Road, EH4 2XU, Edinburgh, UK

    J. R. Gosden & A. R. Mitchell

  4. Department of Molecular Biology and Genetics, Wayne State University, 48201, Detroit, MI, USA

    D. A. Miller

Authors
  1. D. N. Cooper
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  2. S. C. Niemann
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  3. J. R. Gosden
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  4. A. R. Mitchell
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  7. D. A. Miller
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  8. L. Lim
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  9. J. Schmidtke
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Cooper, D.N., Niemann, S.C., Gosden, J.R. et al. Regional localization and characterization of a DNA segment on the long arm of chromosome 21. Hum Genet 75, 129–135 (1987). https://doi.org/10.1007/BF00591073

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  • DOI: https://doi.org/10.1007/BF00591073

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