Summary
Polyacrylamide gel isoelectric focusing followed by electroblotting with enzyme immunoassay was done for the investigation of allotypes of properdin factor B (BF) in serum from 326 Japanese subjects. A new BF F variant tentatively designated BF*Fb1 (b=basic) was detected, the isoelectric point of each band of homozygous BF Fb1 being higher than of BF FF. Family data were in accordance with transmission by mendelian inheritance. The allele frequencies calculated from 326 Japanese subjects were 0.7945, 0.1825, 0.0215, and 0.0015 for BF*S, BF*F, BF*Fb1, and BF*F075, respectively, with that of variant BF*Fb1 being a polymorphic frequency. The distribution of phenotypes fitted the Hardy-Weinberg equilibrium.
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References
Abbal M, Thomsen M, Carubon-Thomsen A, Archambeau J, Calot M, Fathallah D (1985) Two subtypes of BfF by isoelectricfocusing: differential linkage to other HLA markers. Hum Genet 69: 181–183
Alper CA, Boenisch T, Watson L (1972) Genetic polymorphism in human glycine-rich beta-glycoprotein. J Exp Med 135:68–80
David V, Fauchet R, Phengsavath H, Guenet L, Le Gall JY (1983) Properdin factor B (Bf) polymorphism: subtyping of SS phenotypes. Hum Genet 64:189–190
Dykes DD, Polesky HF, Crawford MH (1981) Properdin factor B (Bf) distribution in North and Central American population. Electrophoresis 2:320–323
Geserick G, Patzelt D, Schroder H, Nagai T (1983) Isoelectrofocusing in the study of the Bf system: existence of two common subtypes of the Bf allele. Vox Sang 44:178–182
Hauptmann G, Tongio M, Mayer S (1976) Bf polymorphism: study of a new variant (F0.55). Hum Genet 33:275–278
Hauptmann G, Wertheimer E, Tongio MM, Mayer S (1977) Bf polymorphism: another variant (S0.8). Hum Genet 36:109–111
Horai S, Juji T, Nakajima H (1979) Hyplotype analysis of the linkage group HLA-A:HLA-B: Bf in Japanese. Hum Genet 51:307–314
Mauff G, Hauptmann G, Hitzeroth HW, Gauchel G, Scherz R (1978) The nomenclature of properdin factor B allotypes. Z Immunitätsforsch 154:115–120
Nakamura S, Ooue O, Abe K (1984) Genetic polymorphism of the seventh component of complement in a Japanese population. Hum Genet 66:279–281
Nakamura S, Abe K (1985) Genetic polymorphism of human factor I (C3b inactivator). Hum Genet 71:45–48
Nerl C, O'Neill GJ (1982) Factor B polymorphism in North American Blacks: study of a new variant Bf F1.35. Hum Genet 61:357–359
Suzuki K, Matsumoto H (1986) A new BF variant (F025). Hum Hered 36:336–338
Teisberg P (1970) High voltage agarose gel electrophoresis in the study of C3 polymorphism. Vox Sang 19:47–56
Teng YS, Tan SG (1982) Subtyping of properdin factor B (Bf) by isoelectrofocusing. Hum Hered 32:362–366
Tokunaga K, Araki C, Juji T, Omoto K (1982) Polymorphism of properdin factor B in Japanese. Description of a rare variant and data of association with HLA and C2. Hum Genet 60:42–45
Weidinger S, Schwarzfischer F, Burgemeister R, Cleve H (1984) Two new Bf S subtypes revealed by isoelectric focusing and immunofixation. Hum Genet 68:90–92
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Nakamura, S., Ohue, O. & Abe, K. A new BF F variant by polyacrylamide gel isoelectric focusing. Hum Genet 77, 295–296 (1987). https://doi.org/10.1007/BF00284492
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DOI: https://doi.org/10.1007/BF00284492