Summary
A fragment that contains a (CA)n sequence from the 3′ untranslated region of the dystrophin gene can be amplified by the polymerase chain reaction and shows length polymorphism in a Caucasian population. The two common alleles differ by 4bp. This new genetic marker has a heterozygosity of about 35% and is typed more rapidly than a conventional restriction fragment length polymorphism. Its localisation at the 3′ end of the dystrophin gene makes it a useful tool for diagnostic applications in families with Duchenne/ Becker muscular dystrophy, and for the analysis of intragenic recombination.
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Oudet, C., Heilig, R. & Mandel, J.L. An informative polymorphism detectable by polymerase chain reaction at the 3′ end of the dystrophin gene. Hum Genet 84, 283–285 (1990). https://doi.org/10.1007/BF00200576
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DOI: https://doi.org/10.1007/BF00200576