Abstract
Fluorescence in situ hybridization (FISH) to interphase nuclei was performed to order probes corresponding to bands 11q22-q23 where the ataxia-telangiectasia (AT) gene(s) have been located. Cosmid probes and one phage probe previously localized to this chromosome 11 region by FISH to metaphase chromosomes, were hybridized to interphase nuclei of the somatic cell hybrid J1a, which contains chromosome 11 as the only human chromosome. Two-color FISH was used with a centromeric reference probe marker. The following order was obtained: cen-D11S385 (CJ52.75)-CJ52.3-D11S384 (CJ52.193) CJ52.114-D11S424 (CJ52.77)-D11S132-NCAM-D11S351 (CJ52.208)-tel. The validity of using the centromeric probe was illustrated by showing that a probe corresponding to 11p13 hybridized more closely to the centromere than a probe corresponding to 11q22-q23, and by using cosmids hybridized three by three.
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Brandriff BF, Gordon LA, Tynan KT, Olsen AS, Mohrenweiser HW, Fertitta A, Carrano AV, Trask BJ (1992) Order and genomic distances among members of the carcinoembryonic antigen (CEA) gene family determined by fluorescence in situ hybridization. Genomics 12:773–779
Cherif D, Julier C, Delattre O, Derré J, Lathrop GM, Berger R (1990) Simultaneous localisation of cosmids and chromosome R-banding by fluorescence microscopy: application to regional mapping of human chromosome 11. Proc Natl Acad Sci USA 87:6639–6643
Cornelis F, Cherif D, James M, Tokino T, Davies J, Girault D, Bernard C, Croquette M-F, Theau D, Avet-Loiseau H, Derre J, Litt M, Berger R, Nakamura Y, Lathrop M, Julier C (1991) Precise localisation of a gene responsible for ataxia telangiectasia on chromosome 11q. Cytogenet Cell Genet 58:1957
Foroud T, Wei S, Ziv Y, Sobel E, Lange E, Chao A, Goradia T, Huo Y, Tolun A, Chessa L, Charmley P, Sanal O, Salman N, Julier C, Concannon P, McConville C, Taylor AMR, Shiloh Y, Lange K, Gatti RA (1991) Localization of an ataxia-telangiecta a locus to a 3 cM interval on chromosome 11q23: linkage analysis of 111 families by an international consortium. Am J Hum Genet 49:1263–1279
Gatti RA, Berkel I, Boder E, Braedt G, Charmley P, Concannon P, Ersoy F, Foroud T, Jaspers NGJ, Lange K (1988) Localization of an ataxia-telangiectasia gene to chromosome 11q22–23. Nature 336:577–580
James MR, Richard CW, Julier C, Cornelis F, Cherif D, Berger R, Tokino T, Nakamura Y, Myers R, Cox D, Lathrop GM (1991) Towards a combined meiotic-, radiation-hybrid and YAC based physical map of chromosome 11q22-q23. Cytogenet Cell Genet 58:1964
Julier C, Nakamura Y, Lathrop GM, O'Connell P, Leppert M, Litt M, Mohamdas T, Lalouel JM, White R (1990) A detailed genetic map of the long arm of chromosome 11. Genomics 7:335–345
Kao FT, Jones C, Puck TT (1976) Genetics of somatic mammalian cells: Genetic, immunologic and biochemical analysis with Chinese hamster cell hybrids containing selected human chromosomes. Proc Natl Acad Sci USA 73:193–197
Lawrence JB, Singer RH, McNeil J (1990) Interphase and metaphase resolution of different distances within the human distrophin gene. Science 240:928–932
Lench NJ, Heim RA, Carpenter S, Swift MR (1991) Genetic and physical mapping of DNA markers close to the ataxia-telangiectasia locus. Cytogenet Cell Genet 58:1965
Trask BJ (1992) Gene mapping by in situ hybridization. Curr Opin Genet Dev 1: 82–87
Trask B, Pinkel D, Engh G van den (1989) The proximity of DNA sequences in interphase cell nuclei is correlated to genomic distance and permits ordering of cosmids spanning 250 kilobases pairs. Genomics 5:710–717
Trask BJ, Massa H, Kenwrick S, Gitschier J (1991) Mapping of human chromosome Xq28 by two-color fluorescence in situ hybridization of DNA sequences to interphase cell nuclei. Am J Hum Genet 48:1–15
Trask BJ, Massa HF, Burmeister M (1992) Fluorescence in situ hybridization establishes the order cen-DXS28(C7)-DXS67(B24)-DXS68(L1)-tel in human chromosome Xp21.3. Genomics 13:455–457
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Cherif, D., Der-Sarkissian, H. & Berger, R. Ordering markers in the region of the ataxia-telangiectasia gene (11q22-q23) by fluorescence in situ hybridization (FISH) to interphase nuclei. Hum Genet 93, 1–6 (1994). https://doi.org/10.1007/BF00218903
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DOI: https://doi.org/10.1007/BF00218903