Skip to main content
Log in

Ultrastructure of rat pineal gland after preparation by Freeze-etching technique

  • Published:
Cell and Tissue Research Aims and scope Submit manuscript

Summary

The pineal glands of Wistar rats were fixed in glutaraldehyde and prepared by the freeze-etching technique. With regard to the form and internal structure of pineal cells this technique has revealed nothing essentially new. Synaptic ribbons are very rarely found. A regular diagonal arrangement of membrane attached particles between the Schwann cell and the axon of an autonomic nerve terminal was observed.

Three types of contact areas between pineal cells are described. In the first two types, which occur more frequently, the diameter of the area varied from 1000 to 2500 Å, with irregularly aggregated membrane associated particles. The third type, larger but less frequently found, has a surface area of 0.4 μm2 and much more regularly distributed membrane associated particles (ø 88 Å). These contact areas can be considered to be electric coupling areas between pineal cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Antón-Tay, F.: Pineal-brain relationships. In: The pineal gland (ed. by G.E.W. Wolstenholme and J. Knight), p. 213–220. Edinburgh and London: Churchill Livingstone 1971

    Google Scholar 

  • Dermietzel, R.: Visualization by freeze-fracturing of regular structures in glia cell membranes. Naturwissensohaften 60, 208 (1973)

    Google Scholar 

  • Dermietzel, R., Brettschneider, H.: Eine Untersuchung peripherer vegetativer Nervenfasern des Ductus deferens mit Hilfe der Gefrierätztechnik. Z. Zellforsch. 137, 111–124 (1973)

    Google Scholar 

  • Krstić, R.: A freeze-etching study of the rat pineal gland. Proceedings of the Third European Anatomical Congr., p. 108–109. Manchester, Windsor and London: John B. Reed Ltd. 1973

    Google Scholar 

  • Livingston, R. B., Pfenninger, K., Moor, H., Akert, K.: Specialized paranodal and interparanodal glial-axonal junctions in the peripheral and central nervous system: a freezeetching study. Brain Res. 58, 1–24 (1973)

    Google Scholar 

  • Milin, R., Devečerski, V., Krstić, R.: Corpus pinéale-glande de nature sensoneuroendocrine. C. R. Acad. Sci. (Bosnie et Herzégovine) 37, 69–84 (1969)

    Google Scholar 

  • Moor, H., Mühlethaler, K.: Fine structure in frozen-etched yeast cells. J. Cell Biol. 17, 609–628 (1963)

    Google Scholar 

  • Orci, L., Perrelet, A., Like, A. A.: Fenestrae in the rough endoplasmic reticulum of the exocrine pancreatic cells. J. Cell Biol. 55, 245–249 (1972)

    Google Scholar 

  • Payton, B. W., Bennett, M.V.L., Pappas, G. D.: Permeability and structures of junctional membranes at an electrotonic synapse. Science 166, 1641–1643 (1969)

    Google Scholar 

  • Revel, J. P., Yee, A. G., Hudspeth, A. J.: Gap junctions between electronically coupled cells in tissue culture and in brown fat. Proc. nat. Acad. Sci. (Wash.) 68, 2924–2927 (1971)

    Google Scholar 

  • Reynolds, E.: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 17, 208–212 (1963)

    Google Scholar 

  • Staehelin, L. A.: Three types of gap junctions interconnecting intestinal epithelial cells visualized by freeze-etching. Proc. nat. Acad. Sci. (Wash.) 69, 1318–1321 (1972)

    Google Scholar 

  • Wolfe, B. E.: The epiphyseal cell: an electron-microscopic study of its intercellular relationships and intercellular morphology in the pineal body of the albino rat. In: Structure and function of the epiphysis cerebri (ed. by J. Ariëns Kappers and J. P. Schadé), p. 332–376. Amsterdam-London New-York: Elsevier 1965

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Krstić, R. Ultrastructure of rat pineal gland after preparation by Freeze-etching technique. Cell Tissue Res. 148, 371–379 (1974). https://doi.org/10.1007/BF00224264

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00224264

Key words

Navigation