Skip to main content
SpringerLink
Log in

Studies on the juxtaglomerular apparatus

III. Quantitative morphology after treatment with deoxycorticosterone (DOC)

  • Published:
Cell and Tissue Research Aims and scope Submit manuscript

Summary

Quantitative morphological analysis of the Juxtaglomerular apparatus of rats treated with deoxycorticosterone (DOC) and salt revealed hypoplasia of the epithelioid cells as a concomitant of the inhibition of renin synthesis. The granular content of the Juxtaglomerular cells was significantly decreased, while the nucleus-to-cytoplasm ratio and the volume density of leiomyofilaments in the epithelioid cells increased. No morphological alterations were observed in the remaining constituents of the Juxtaglomerular apparatus, including the macula densa. DOC-treatment also induced the appearance of inclusions in the podocytes in the cells of the mesangium, and in the cells of Bowman's capsule. The possible mechanisms of degranulation of the Juxtaglomerular cells after treatment with DOC are discussed. The ultrastructural appearance of the epithelioid cells after treatment with DOC corresponds to an inhibition of secretion, since the rough endoplasmic reticulum and the Golgi apparatus undergo marked regressive changes.

Zusammenfassung

Die quantitativ-morphologische Analyse des juxtaglomerulären Apparates nach DOC-Behandlung zeigte, daß mit der Hemmung der Reninbildung eine Hypoplasie der epitheloiden Zellen des juxtaglomerulären Apparates einhergeht. Der Granulagehalt der juxtaglomerulären Zellen nahm ab; die Kern-Plasma-Relation änderte sich und die Leiomyofibrillen in den Epitheloidzellen nahmen zu. An den übrigen Anteilen des juxtaglomerulären Apparates, insbesondere der Macula densa, wurden keine morphologischen Veränderungen beobachtet. Die DOC-Behandlung führte darüber hinaus zu einer Bildung von Einschlüssen in den Podocyten, den Mesangiumzellen und den Zellen der Bowman-Membran. Die verschiedenen Möglichkeiten, über welche Faktoren die juxtaglomerulären Zellen nach DOC-Behandlung degranuliert werden, werden diskutiert. Das ultrastrukturelle Bild der Epitheloidzellen nach DOC-Behandlung entspricht deutlich einer gehemmten Sekretion, da das Ergastoplasma und der Golgi-Apparat ganz besonders stark zurückgebildet werden.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Barajas, L., Latta, H.: The juxtaglomerular apparatus in adrenalectomized rats. Light and electron microscopic observations. Lab. Invest. 12, 1046–1059 (1963)

    Google Scholar 

  • Bernardis, L. L., Kamura, S.: Blood pressure and dietary sodium as regulators of juxtaglomerular cell function. Fed. Proc. 23, 445 (1965)

    Google Scholar 

  • Bohle, A., Sitte, H.: Der juxtaglomeruläre Apparat der Niere. In: Aktuelle Probleme der Nephrologie. IV. Symp. Ges. Nephrologie 1966, p. 3. Berlin-Heidelberg-New York: Springer 1966

    Google Scholar 

  • Bohle, A., Tomsche, U.: Das Verhalten der epitheloiden Zellen der Vasa afferentia der Nierenkörperchen bei experimenteller Hypotonie. Beitr. path. Anat. 113, 399–413 (1953)

    Google Scholar 

  • Bucher, O., Riedel, B., Erkocak, A.: Die epitheloiden Zellen des juxtaglomerulären Apparates der Meerschweinchenniere vor und nach bilateraler Adrenalektomie. Verh. Anat. Ges., Anat. Anz., Erg.-H. 120, 243–249 (1967)

    Google Scholar 

  • Christlieb, A. R., Amsterdam, E. A., Hickler, R. B.: Suppression of murine renal renin activity in the absence of sodium retention with desoxycorticosterone acetate but not with depot desoxycorticosterone. Amer. J. Med. Sci. 263, 457–464 (1972)

    Google Scholar 

  • Dunihue, F. W.: The effect of bilateral adrenalectomy on the juxtaglomerular apparatus. Anat. Rec. 96, 536–549 (1946)

    Google Scholar 

  • Dunihue, F. W.: The effect of adrenal insufficiency and of desoxycorticosterone acetate on the juxtaglomerular apparatus. Anat. Rec. 103, 442–449 (1949)

    Google Scholar 

  • Dunihue, F. W., Boldosser, W. G.: Observations on the similarity of mesangial to juxtaglomerular cells. Lab. Invest. 12, 1228–1239 (1963)

    Google Scholar 

  • Dunihue, F. W., Robertson, W. van: The effect of desoxycorticosterone acetate and of sodium on the juxtaglomerular apparatus. Endocrinology 61, 293–299 (1957)

    Google Scholar 

  • Fischer, E. R., Tamura, M.: Effect of aldosterone on juxtaglomerular index and Zona glomerulosa of normotensive and hypertensive rats. Proc. Soc. exp. Biol. (N.Y.) 118, 402–405 (1965)

    Google Scholar 

  • Gross, F.: Renin und Hypertensin, physiologische oder pathologische Wirkstoffe? Klin. Wschr. 36, 693–706 (1958)

    Google Scholar 

  • Gross, F., Brunner, H., Ziegler, M.: Renin-angiotensin system, aldosterone, and sodium balance. Recent Progr. Hormone Res. 21, 119–167 (1965)

    Google Scholar 

  • Gross, F., Sulser, F.: Pressorische Substanzen in den Nieren experimentell hypertonischer Ratten. Naunyn-Schmiedebergs Arch. exp. Path. Pharmak. 229, 374–380 (1956)

    Google Scholar 

  • Hartroft, P. M., Hartroft, W. S.: Studies on renal juxtaglomerular cells. I. Variations produced by sodium chloride and desoxycorticosterone acetate. J. exp. Med. 97, 415–427 (1953)

    Google Scholar 

  • Hatt, P. Y.: L'appareil juxtaglomerulaire dans l'hypertension artérielle experiméntelle. Rev. franç. Étud. clin. biol. 8, 358–365 (1963)

    Google Scholar 

  • Hatt, P. Y.: The juxtaglomérular apparatus. In: Ultrastructure of the kidney, A. J. Dalton and F. Haguenau, eds., p. 101–141. New York: Academic Press 1967

    Google Scholar 

  • Meyer, D., Mittmeyer, P. J.: Relationship between serum sodium concentration, hematocrit, and juxtaglomerular granulation index of the mouse kidney. V. Symp. Ges. Nephr., Lausanne 1967. In: G. Peters and F. Roch-Ramel, ed Progress in nephrology, p. 322–341. Berlin-Heidelberg-New York: Springer 1969

    Google Scholar 

  • Peter, St., Lazar, J., Gross, F.: Untersuchungen der juxtaglomerulären Zellen bei adrenalektomierten Ratten. Verh. Anat. Ges., Anat. Anz., Erg.-H. 67, 161–167 (1973)

    Google Scholar 

  • Peter, St., Lazar, J., Gross, F., Forssmann, W. G.: Studies on the juxtaglomerular apparatus. II. Quantitative morphology after adrenalectomy. Cell Tiss. Res. 151, 457–469 (1974)

    Google Scholar 

  • Pitcock, J. A., Hartroft, P. M.: Pressor activity (renin) of kidneys from sodium deficient rats and correlation with granulation of juxtaglomerular cells. Fed. Proc. 18, 500 fp. (1959)

    Google Scholar 

  • Schaechtelin, G., Riedel, B.: Renin-Aktivität in Nieren ohne “Renin-Granula”. Naunyn-Schmiedebergs Arch. Pharmak. exp. Path. 255, 65–66 (1966)

    Google Scholar 

  • Tobian, L. A.: Physiology of the juxtaglomerular cells. Ann. intern. Med. 52, 395–410 (1960)

    Google Scholar 

  • Tobian, L. A.: Renin release and its role in renal function and the control of salt balance and arterial pressure. Fed. Proc. 26, 48–54 (1967)

    Google Scholar 

  • Tobian, L. A., Janecek, J., Tomboulian, A.: Correlation between granulation of juxtaglomerular cells and extractable renin in rats with experimental hypertension. Proc. Soc. exp. Biol. (N.Y.) 100, 94–96 (1959)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Anatomy, University of Heidelberg, Germany

    St. Peter, F. Gross & W. G. Forssmann

  2. Department of Pharmacology, University of Heidelberg, Germany

    J. Lazar

Authors
  1. St. Peter
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. Lazar
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. F. Gross
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. W. G. Forssmann
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

These studies were supported by the “Deutsche Forschungsgemeinschaft” (SFB90, “Cardiovasculäres System”). — The authors acknowledge the valuable assistance of Miss C. Denereaz and Miss K. Kahl.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Peter, S., Lazar, J., Gross, F. et al. Studies on the juxtaglomerular apparatus. Cell Tissue Res. 151, 471–480 (1974). https://doi.org/10.1007/BF00222992

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00222992

Key words

Search

Navigation