Summary
The fine structure of the secretory epithelial cells of rat's ventral prostate has been studied following organ culture. Culturing with either testosterone or insulin alone, and with the two hormones combined, were carried out to investigate how insulin modifies the action of testosterone on the maintenance of cellular integrity. After 4 days in hormone-free culture, the secretory epithelial cells showed signs of cellular atrophy and regression, involving loss of the apical microvilli, absence of the apical secretory vacuoles, atrophy of the Golgi apparatus, decrease in rough endoplasmic reticulum and the appearance of autophagic vacuoles. The presence in the medium of either testosterone or insulin alone, or combined, prevented cellular atrophy and regression. The best maintenance of cellular integrity was obtained in a culture containing both hormones. The effects of insulin was approximately equivalent to those of testosterone in the maintenance of cellular integrity.
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Angervall, L., Hesselsjö, R., Nilsson, S., Tisell, L.E.: Action of testosterone on ventral prostate, dorsolateral prostate, coagulating glands and seminal vesicle of castrated alloxan-diabetic rats. Diabetologia 3, 395–401 (1967)
Brandes, D.: The fine structure and histochemistry of prostatic glands in relation to sex hormones. Int. Rev. Cytol. 20, 207–276 (1966)
Brandes, D., Groth, D.P.: Functional ultrastructure of rat prostatic epithelium. Nat. Cancer Inst. Monogr. 12, 47–62 (1963)
Brandes, D., Groth, D.P., Gyorky, F.: Occurrence of lysosomes in the prostatic epithelium of castrate rats. Exp. Cell Res. 28, 61–68 (1962)
Calame, S.S., Lostroh, A.J.: Effect of insulin and lack effect of testosterone on the protein of ventral prostate from castrated mice maintained as organ cultures. Endocrinology 75, 451–454 (1964)
Chase, M.D., Geschwind, J., Bern, G.A.: Synergistic role of prolactin in response of male rat sex accessories to androgen. Proc. Soc. exp. Biol. (N.Y.) 94, 680 (1957)
Dahl, E., Kjaerheim, Å.: The ultrastructure of the accessory sex organs of the male rat. 11. The postcastration involution of the ventral, lateral and the dorsal prostate. Z. Zellforsch. 144, 167–178 (1973)
Dahl, E., Kjaerheim, Å., Tveter, K.J.: The ultrastructure of the accessory sex organs of the male rat. 1. Normal structure. Z. Zellforsch. 137, 345–359 (1973)
DiDio, L.J.A.: Correlative light and electron microscopy of the normal prostatic ventral lobe in rats. Anat. Anz. 128, 170–190 (1971)
Flickinger, C.J.: Ultrastructural observations on the postnatal development of the rat prostate. Z. Zellforsch. 113, 157–173 (1971)
Flickinger, C.J.: Protein secretion in the rat ventral prostate and the relation of Golgi vesicles, cisternae and vacuoles, as studied by electron microscope radioautography. Anat. Rec. 180, 427–448 (1974)
Gittinger, J.W., Lasnitzki, I.: The effect of testosterone and testosterone metabolites on the fine structure of the rat prostate gland in organ culture. J. Endocr. 52, 459–464 (1972)
Grayhack, J.T.: Pituitary factors influencing growth of the prostate. Nat. Cancer Inst. Monogr. 12, 189–199 (1963)
Harkin, J.C.: An electron microscopic study of the castration changes in the rat prostate. Endocrinology 60, 185–199 (1957)
Harkin, J.C.: Prostatic ultrastructure. Nat. Cancer Inst. Monogr. 12, 85–97 (1963)
Helminen, H.J., Ericsson, J.L.E.: On the mechanism of lysosomal enzyme secretion. Electron microscopic and histochemical studies on the epithelial cells of the rat's ventral prostate lobe. J. Ultrastruct. Res. 33, 528–549 (1970)
Helminen, H.J., Ericsson, J.L.E.: Ultrastructural studies on prostatic involution in the rat. Mechanism of autophagy in epithelial cells, with special reference to the rough endoplasmic reticulum. J. Ultrastruct. Res. 36, 708–724 (1971)
Helminen, H.J., Ericsson, J.L.E.: Ultrastructural studies on prostatic involution in the rat. Changes in the secretory pathways. J. Ultrastruct. Res. 40, 152–166 (1972)
Ichihara, I., Pelliniemi, L.J.: Ultrastructure of the basal cell and the acinar capsule of rat ventral prostate. Anat. Anz. 138, 355–364 (1975)
Ichihara, I., Santti, R.S., Pelliniemi, L.J.: Effect of testosterone, hydrocortisone and insulin on the fine structure of the epithelium of rat ventral prostate in organ culture. Z. Zellforsch. 143, 425–438 (1973)
Klaiber, E.L., Lloyd, C., Salomon, A., Broverman, D.M.: Augumentation of the effects of testosterone propionate by Cortisol in immature male rats. Endocrinology 83, 387–389 (1968)
Lostroh, A.J.: Regulation by testosterone and insulin of citrate secretion and protein synthesis in explanted mouse prostates. Proc. nat. Acad. Sci. (Wash.) 60, 1312–1318 (1968)
Lostroh, A.J.: Effects of testosterone and insulin in vitro on maintenance and repair of the secretory epithelium of the mouse prostate. Endocrinology 88, 500–503 (1971)
Luft, J.H.: Improvements in epoxy resin embedding methods. J. biophys. biochem. Cytol. 9, 409–414 (1961)
Sakurai, S.: An electron microscopic study of the rat prostate, with special reference to the effect of hypophysectomy. 1. Report; A supplemental description on the fine structure of normal rat prostate. Jap. J. Urol. 60, 1–14 (1969)
Santti, R.S., Johansson, R.: Action of cyproterone on the rat prostate in organ culture. Gynec. Invest. 2, 276–281 (1972)
Santti, R.S., Johansson, R.: Some biochemical effects of insulin and steroid hormones on prostate in organ cultures. Exp. Cell Res. 77, 111–120 (1973)
Surfin, G., Prutkin, L.: Experimental diabetes and the response of the sex accessory organs of the castrate male rat to testosterone propionate. Invest. Urol. 11, 361–369 (1974)
Szirmai, J.A., Linde, P.C. van der: Effect of castration on the endoplasmic reticulum of the seminal vesicle and other target epithelia in the rat. J. Ultrastruct. Res. 12, 380–395 (1965)
Tisell, L.E.: Effect of cortisone on the growth of the ventral prostate, the dorsolateral prostate, the coagulating glands and the seminal vesicles in castrated adrenalectomized and castrated nonadrenalectomized rats. Acta endocr. (Kbh.) 64, 637–655 (1970)
Trowell, O.A.: The culture of mature organs in a synthetic medium. Exp. Cell Res. 16, 118–147 (1959)
Venable, J.H., Coggeshall, R.L.: A simplified lead citrate stain for use in electron microscopy. J. Cell Biol. 25, 407–408 (1965)
Watson, M.L.: Staining of tissue sections for electron microscopy with heavy metals. J. biophys. biochem. Cytol. 4, 475–478 (1958)
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Ichihara, I. Some ultrastructural effects of testosterone and insulin on the ventral prostate of rats in organ culture. Cell Tissue Res. 181, 327–337 (1977). https://doi.org/10.1007/BF00223108
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DOI: https://doi.org/10.1007/BF00223108