Summary
The ultrastructure of the pineal parenchymal cells, pinealocytes and glia-like cells, of the Chinese hamster (Cricetulus griseus) is described. Pinealocyte nuclei contain spindle-shaped inclusions consisting of bundles of closely packed parallel filaments of about 8 nm in thickness. Mitochondria contain lamellar and tubular cristae. Smaller and larger mitochondria are usually localized separately in individual pinealocytes. Flattened mitochondria contain two to four closely arranged, unusual lamellar cristae, which lie parallel to the surface of the mitochondria. These cristae exhibit particular structures. Rough endoplasmic reticulum is frequently found in close association with such flattened mitochondria. Tubular expansions of the outer membrane of mitochondria occur frequently. The pinealocyte cytoplasm contains stacks of flattened cisternae, which are continuous with both smooth and rough endoplasmic reticulum. Pinealocyte cell bodies and processes contain granulated vesicles. Although larger granulated vesicles measuring up to 300 nm in diameter occasionally occur, smaller granulated vesicles (about 100 nm in diameter) and larger ones are usually localized separately in individual cells. Vacuoles containing flocculent material are found in groups in close association with rough endoplasmic reticulum. Glia-like cells with darker cytoplasm and nuclei lie surrounding pericapillary or intercellular spaces. The glia-like cells are characterized by the presence of abundant lipid droplets and occasional pigment granules. Bundles of filaments run parallel to the long axis of the processes of the glia-like cells.
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References
Arstila AU, Hopsu VK (1967) Nuclear and cytoplasmic microfilaments in the pineal chief cells of the rat. Z Zellforsch 80:22–28
Barratt GF, Nadakavukaren MJ, Frehn JL (1977) Effect of melatonin implants on gonadal weights and pineal gland fine structure of the golden hamster. Tissue Cell 9:335–345
Benson B, Krasovich M (1977) Circadian rhythm in the number of granulated vesicles in the pinealocytes of mice. Effects of sympathectomy and melatonin treatment. Cell Tissue Res 184:499–506
Bucana CD, Nadakavukaren MJ, Frehn JL (1971) Annulate lamellae in hamster pineal gland. Tissue Cell 3:405–412
Bucana CD, Nadakavukaren MJ, Frehn JL (1973) Ultrastructural features of the pineal gland from cold-exposed golden hamsters. J Neurocytol 2:237–247
Bucana CD, Nadakavukaren MJ, Frehn JL (1974) Novel features of hamster pinealocyte ultrastructure. Tissue Cell 6:85–93
Clabough JW (1971) Ultrastructural features of the pineal gland in normal and light deprived golden hamsters. Z Zellforsch 114:151–164
Clattenburg RE, Singh RP, Montemurro DG (1972) Intranuclear filamentous inclusions in neurons of the rabbit hypothalamus. J Ultrastruct Res 39:549–555
David S, Nathaniel EJH (1978) Intranuclear inclusions in the developing neurons of the rat cuneate nuclei. Cell Tissue Res 193:525–532
Feldman ML, Peters A (1972) Intranuclear rods and sheets in rat cochlear nucleus. J Neurocytol 1:109–127
Field EJ, Peat A (1971) Intranuclear inclusions in neurones and glia: a study in the ageing mouse. Gerontologia 17:129–138
Gregorek JC, Seibel HR, Reiter RJ (1977) The pineal complex and its relationship to other epithalamic structure. Acta Anat 99:425–434
Hewing M (1978) A liquor contacting area in the pineal recess of the golden hamster (Mesocricetus auratus). Anat Embryol 153:295–304
Hewing M (1980) Synaptic ribbons in the pineal system of normal and light deprived golden hamsters. Anat Embryol 159:71–80
Ito T, Matsushima S (1968) Electron microscopic observations on the mouse pineal, with particular emphasis on its secretory nature. Arch Histol Jpn 30:1–15
Johnson JE Jr (1980) Fine structural alterations in the aging rat pineal gland. Exp Aging Res 6:189–211
Juillard MT, Collin JP (1980) Pools of serotonin in the pineal gland of the mouse: the mammalian pinealocyte as a component of the diffuse neuroendocrine system. Cell Tissue Res 213:273–291
Kachi T (1979) Demonstration of circadian rhythm in granular vesicle number in pinealocytes of mice and the effect of light: semiquantitative electron microscopic study. J Anat 129:603–614
Kessel RG (1968) Annulate lamellae. J Ultrastruct Res, Suppl 10:1–76
Lin H-S (1965) Microcylinders within mitochondrial cristae in the rat pinealocyte. J Cell Biol 25:435–441
Lin H-S, Hwang B-H, Tseng C-Y (1975) Fine structural changes in the hamster pineal gland after blinding and superior cervical ganglionectomy. Cell Tissue Res 158:285–299
Matsushima S, Morisawa Y (1981) Quantitative morphological studies on pinealocytes of the Chinese hamster, Cricetulus griseus. Jikeikai Med J, Suppl 1, 28:29–34
Matsushima S, Reiter RJ (1975) Comparative ultrastructural studies of the pineal gland of rodents. In: Hess M (ed) Ultrastructure of endocrine and reproductive organs. John Wiley and Sons, New York, pp 335–356
Matsushima S, Kachi T, Mukai S, Morisawa Y (1977) Functional relationships between sympathetic nerves and pinealocytes in the mouse pineal: quantitative electron microscopic observations. Arch Histol Jpn, Suppl 40:279–291
Nathaniel DR (1976) Helical inclusions and atypical cristae in the mitochondria of the rabbit thyroid gland. J Ultrastruct Res 57:194–203
Pévet P (1979) Secretory processes in the mammalian pinealocyte under natural and experimental conditions. In: Ariëns Kappers J, Pévet P (eds) Progress in brain research. The pineal gland of vertebrates including man. Elsevier/North Holland, Amsterdam, Vol 52, pp 149–194
Pévet P, Kuyper MA (1978) The ultrastructure of pinealocytes in the golden mole (Amblysomus hottentotus) with special reference to the granular vesicles. Cell Tissue Res 191:39–56
Pévet P, Yadav M (1980) The pineal gland of equatorial mammals. I. The pinealocytes of the Malaysian rat (Rattus sabanus). Cell Tissue Res 210:417–433
Seite R, Leonetti J, Luciani-Vuillet J (1977) Cyclic AMP and ultrastructural organization of the nerve cell nucleus: Stimulation of nuclear microtubules and microfilaments assembly in sympathetic neurons. Brain Res 124:41–51
Sheridan MN (1975) Pineal gland fine structure: dense cored vesicles. In: Knigge KM, Scott DE, Kobayashi H, Ishii S (eds) Brain-endocrine interaction. II. The ventricular system in neuroendocrine mechanisms. S Karger, Basel, pp 324–336
Sheridan MN, Reiter RJ (1968) The fine structure of the hamster pineal gland. Am J Anat 122:357–376
Sheridan MN, Reiter RJ (1970) Observations on the pineal system in the hamster. II. Fine structure of the deep pineal. J Morphol 131:163–178
Sheridan MN, Reiter RJ (1973) The fine structure of the pineal gland in the pocket gopher, Geomys bursarius. Am J Anat 136:363–382
Sheridan MN, Sladek JR Jr (1975) Histofluorescence and ultrastructural analysis of hamster and monkey pineal. Cell Tissue Res 164:145–152
Sheridan MN, Walker SH (1975) Fine structural features of pineal gland development. In: Hess M (ed) Ultrastructure of endocrine and reproductive organs. John Wiley and Sons, New York, pp 317–333
Siegesmund KA, Dutta CR, Fox CA (1964) The ultrastructure of the intranuclear rodlet in certain nerve cells. J Anat 98:93–97
Spacek J, Lieberman AR (1980) Relationships between mitochondrial outer membranes and agranular reticulum in nervous tissue: Ultrastructural observations and a new interpretation. J Cell Sci 46:129–147
Spurr AR (1969) A low viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res 26:31–43
Theron JJ, Biagio R, Meyer AC, Boekkooi S (1979) Microfilaments, the smooth endoplasmic reticulum and synaptic ribbon fields in the pinealocytes of the baboon (Papio ursinus). Am J Anat 154:151–162
Willey TJ, Schultz RL (1971) Intranuclear inclusions in neurons of the cat primary olfactory system. Brain Res 29:31–45
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Acknowledgements. Professor Kazuya Mikamo, Department of Biological Sciences, Asahikawa Medical College, is gratefully acknowledged for providing the animals. We wish to thank Professor Russel J. Reiter, University of Texas Health Science Center at San Antonio, for the review of the manuscript
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Matsushima, S., Morisawa, Y. Ultrastructural observations on the pineal gland of the Chinese hamster, Cricetulus griseus . Cell Tissue Res. 222, 531–546 (1982). https://doi.org/10.1007/BF00213853
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DOI: https://doi.org/10.1007/BF00213853