Summary
The influence of adrenalectomy and administration of hypertonic saline on the amount of vasopressin, oxytocin, and neurophysin contained in the median eminence and the neural lobe of rats was studied by means of the following methods: (i) morphometric and microphotometric analyses of aldehyde fuchsin-stained histological sections of the neurohypophysis; (ii) immunohistochemical demonstration of vasopressin, oxytocin, and neurophysin in the neurohypophysis, and (iii) radioimmunological measurement of vasopressin and oxytocin in extracts of the median eminence and the neural lobe.
Adrenalectomy increases the amount of vasopressin and neurophysin in the external layer of the median eminence but does not change the content of oxytocin. It has no influence on the amount of vasopressin, oxytocin, and neurophysin demonstrable in the inner layer of the median eminence and in the neural lobe two weeks after the operation.
Hypertonic saline markedly diminishes the vasopressin, oxytocin, and neurophysin content of the inner layer of the median eminence and the neural lobe but reduces only slightly, if at all, the amount of vasopressin and neurophysin in the outer layer of the median eminence.
The findings support the concept that osmotic stress reduces only the vasopressin and oxytocin content of the hypothalamus-neural lobe system and has no or only little influence on the vasopressin content of the outer layer of the median eminence.
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References
Bock R, Salland T, Schwabedal PE, Watkins WB (1976) Histochemical and immunohistochemical properties of the CRF-granules and other “Gomori-positive” substances of the rat. Histochemistry 46:81–105
Bock R, Detzer K, Leicht E, Röder R (1980) Functional difference between “classical” neurosecretory material and vasopressin-like substances of the outer layer of the median eminence. Cell Tissue Res 212:257–277
Brinkmann H, Bock R (1970) Quantitative Veränderungen “Gomori-positiver” Substanzen in Infundibulum und Hypophysenhinterlappen der Ratte nach Adrenalektomie und Kochsalz-oder Durstbelastung. J Neuro Visceral Relat 32:48–64
Brownstein M, Russel JT, Gainer H (1980) Synthesis, transport and release of posterior pituitary hormones. Science 207:373–378
Burlet A, Chateau M, Czernichow P (1979) Infundibular localization of vasopressin, oxytocin and neurophysins in the rat; its relationships with corticotrope function. Brain Res 168:275–286
Defendini R, Zimmerman EA (1978) The magnocellular neurosecretory system of the mammalian hypothalamus. In: Reichlin S, Baldessarini RJ, Martin JB (eds) The hypothalamus. Raven-Press, New York, pp 137–152
Dierickx K, Vandesande F, Mey J de (1976) Identification in the external region of the rat median eminence, of separate neurophysin-vasopressin and neurophysin-oxytocin containing nerve fibres. Cell Tissue Res 168:141–151
George JM (1976) Vasopressin and oxytocin are depleted from rat hypothalamic nuclei after oral hypertonic saline. Science 193:146–148
Hashimoto K, Yunoki S, Kageyama J, Ohno N, Takahara J, Ofuju T (1981) Vasopressin and CRF-ACTH in adrenalectomized and dexamethasone-treated rats. Neuroendocrinology 32:87–91
Jackson R, George JM (1980) Oxytocin in microdissected hypothalamic nuclei. Significant differences between prepubertal and sexually mature female rats. Neuroendocrinology 31:158–160
Jones CW, Pickering BT (1969) The effects of water deprivation and sodium chloride imbibition on the hormone content of the neurohypophysis of the rat. J Physiol 203:449–458
Lang RE, Rascher W, Unger Th, Ganten D (1981a) Reduced content of vasopressin in the brain of spontaneously hypertensive as compared to normotensive rats. Neuroscience Letters 23:199–202
Lang RE, Rascher W, Heil J, Unger Th, Wiedemann G, Ganten D (1981b) Angiotensin stimulates oxytocin release. Life Sciences 29:1425–1428
Livingstone A (1973) Ultrastructure of the rat neural lobe during recovery from hypertonic saline treatment. Z Zellforsch 137:361–374
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
Sachs L (1968) Statistische Auswertungsmethoden. Springer, Berlin Heidelberg New York
Schwabedal PE, Partenheimer U, Bock R (1976) Influence of sodium chloride on the amount of CRF-granules and “classical” neurosecretory material (NSM) in the neurohypophysis of bilaterally adrenalectomized rats. Anat Embryol 149:307–313
Sofroniew MV, Weindl A, Wetzstein R (1977) Immunoperoxidase staining of vasopressin in the rat median eminence following adrenalectomy and steroid substitution. Acta Endocrinol Suppl 212:72
Sternberger LA, Hardy PH, Cuculis JJ, Meyer HG (1970) The unlabeled antibody enzyme method of immunohistochemistry. Preparation and properties of soluble antigen-antibody complex (horse-radish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes. J Histochem Cytochem 18:315–333
Stillman MA, Recht LD, Rosario SL, Seif SM, Robinson AG, Zimmerman EA (1977) The effects of adrenalectomy and glucocorticoid replacement on vasopressin and vasopressin-neurophysin in the zona externa of the median eminence of the rat. Endocrinol 101:42–49
Stöhr PhA (1969) Über quantitative Veränderungen “gomoripositiver” Substanzen in Infundibulum und Hypophysenhinterlappen der Ratte nach beidseitiger Adrenalektomie. Z Zellforsch 94:425–433
Vandesande F, Dierickx K (1975) Identification of the vasopressin producing and of the oxytocin producing neurons in the hypothalamic magnocellular neurosecretory system of the rat. Cell Tissue Res 164:153–162
Vandesande F, Dierickx K, Mey J de (1977) The origin of the vasopressinergic and oxytocinergic fibres of the external region of the median eminence of the rat hypophysis. Cell Tissue Res 180:443–452
Watkins WB, Schwabedal PE, Bock R (1974) Immunohistochemical demonstration of a CRF-associated neurophysin in the external zone of the rat median eminence. Cell Tissue Res 152:411–421
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Supported by the Deutsche Forschungsgemeinschaft (Bo 392/6-41 and SFB90, Cardiovasculäres System, A 53)
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Bock, R., Detzer, K., Geiger, A. et al. Effect of adrenalectomy and administration of hypertonic saline on the content of aldehyde fuchsin-positive neurosecretory material and posterior lobe hormones in the median eminence and the neural lobe of rats. Cell Tissue Res. 228, 127–138 (1983). https://doi.org/10.1007/BF00206271
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DOI: https://doi.org/10.1007/BF00206271