Summary
After 2 h intraocular injections of 19 and 190 nmoles ibotenic acid in the rat retina produced an intensive vacuolization of the inner plexiform layer and cellular alterations, in the inner nuclear layer and ganglion cell layer. These alterations consisted of either cytoplasmic swelling accompanied by clumping of the nuclear chromatin or darkening of the cytoplasm along with nuclear condensation. A week later the retinas were thinner than the controls due to the disappearance of the affected cells. Pre-treatment with diazepam prevents the morphological alterations induced by 19 nmoles ibotenic acid; mainly the swelling, which was completely prevented, while the darkening was reduced drastically, although some vacuolization of the inner plexiform layer is still present.
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References
Ben-Ari Y, Lagowska Y, Le Gal La Salle G, Tremblay E, Ottersen OP, Naquet R (1978) Diazepam pretreatment reduces distant hippocampal damage induced by intra-amygdaloid injections of kainic acid. Eur J Pharmacol 52:419–420
Coyle JT, Biziere K, Schwarcz R (1978) Neurotoxicity of excitatory amino acids in the neural retina. In: McGeer EG et al (Ed) Kainic acid as a tool in neurobiology. Raven Press, New York pp 177–188
Curtis DR, Lodge D, McLennan H (1979) The excitation and depression of spinal neurons by ibotenic acid. J Physiol (Lond) 291:19–28
Davies J, Evans RH, Francis AA, Jones AW, Watkins JC (1980) Excitatory amino acids receptors in the vertebrate central nervous system. In: Littauer UZ et al (Ed) Neurotransmitters and their receptors. John Wiley and Son, New York pp 333–347
Garey LJ, Hornung JP (1980) The use of ibotenic acid lesions for light and electron microscopic study of anterograde degeneration in the visual patway of the cat. Neurosci Lett 19:117–123
Gomez-Ramos P, Perez-Rico C (1983) Morphological alterations of retinal cells accompanying inhibition of axonal transport by kainic acid. Exp Eye Res 36:299–304
Goto M, Inomata N, Ono H, Saito KI, Fukuda H (1981) Changes in the electroretinogram and neurochemical aspects of GABA-ergic neurons of retina after intraocular injection of kainic acid in rats. Brain Res 211:293–305
Hampton CK, Garcia C, Redburn DA (1981) Localization of kainic acid-sensitive cells in mammalian retina. J Neurosci Res 6:99–111
Imperato A, Porceddu ML, Morelli M, Fossarello M, Di Chiara G (1981) Benzodiazepines prevent kainate-induced loss of GABA-ergic and cholinergic neurons in the chick retina. Brain Res 213:205–210
Ingham CA, Morgan IG (1983) Dose-dependent effects of intravitreal kainic acid on specific cell types in chicken retina. Neuroscience 9:165–181
Köhler C, Schwarcz R, Fuxe K (1979) Intrahippocampal injections of ibotenic acid provide histological evidence for a neurotoxic mechanism different from kainic acid. Neurosci Lett 15:223–228
Nistri A (1981) Excitatory and inhibitory actions of ibotenic acid on frog spinal motoneurons in vitro. Brain Res 208:397–408
Olney JW (1978) Neurotoxicity of excitatory amino acids. In: McGeer EG et al. (Ed) Kainic acid as a tool in Neurobiology. Raven Press, New York pp 95–121
Puil E (1981) Ibotenic acid: its excitatory and possibly sedative actions in cerebral cortex. Can J Physiol Pharmacol 59:1025–1030
Schwarcz R, Coyle JT (1977) Kainic acid: neurotoxic effects after intraocular injection. Invest Ophthalmol Vis Sci 16:141–148
Schwarcz T, Hökfelt T, Fuxe K, Jonsson G, Goldstein M, Terenius L (1979) Ibotenic acid-induced neuronal degeneration: A morphological and neurochemical study. Exp Brain Res 37:199–216
Yazulla S, Kleinschmidt J (1980) The effects of intraocular injection of kainic acid on the synaptic organization of the goldfish retina. Brain Res 182:287–301
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Perez-Rico, C., Gomez-Ramos, P. Histological study of ibotenic acid-induced modifications of rat retina and their attenuation by diazepam. Cell Tissue Res. 238, 81–85 (1984). https://doi.org/10.1007/BF00215147
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DOI: https://doi.org/10.1007/BF00215147