Summary
Capsaicin injected into the scrotal skin of rats was observed to induce a decrease in the amount of horseradish peroxidase (HRP) transported in the pudendal nerve to the sixth lumbar dorsal root ganglion on the pretreated side. This was seen as a decrease in the number of HRP-labelled neurones compared to the control side. A morphometric study confirmed that the effect of capsaicin was exerted predominantly on the small neurones. Injection of nerve growth factor (NGF) into the pudendal nerve prevented the deleterious effects of capsaicin, thereby suggesting a possible site of action and mechanism for the effect of capsaicin on peripheral nerves.
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References
Almon RR, McClure WO (1974) The effect of nerve growth factor (NGF) upon axoplasmic transport in sympathetic neurons of the mouse. Brain Res 74:255–267
Fitzgerald M (1983) Capsaicin and sensory neurones — a review. Pain 15:109–130
Gamse R, Holzer P, Lembeck F (1980) Decrease of substance P in primary afferent neurones and impairment of neurogenic plasma extravasation by capsaicin. Brit J Pharmacol 68:207–213
Gamse R, Molnar A, Lembeck F (1979) Substance P release from spinal cord slices by capsaicin. Life Sci 25:629–636
Gamse R, Petsche U, Lembeck F, Jancsó G (1982) Capsaicin applied to peripheral nerve inhibits axoplasmic transport of substance P and somatostatin. Brain Res 239:447–462
Jessell TM, Iversen LL, Cuello AC (1978) Capsaicin-induced depletion of substance P from primary sensory neurones. Brain Res 152:183–188
Lawson SN (1979) The postnatal development of large light and small dark neurones in mouse dorsal root ganglia: A Statistical analysis of cell numbers and size. J Neurocytol 8:275–294
Lawson SN, Nickels SM (1980) The use of morphometric techniques to analyse the effect of neonatal capsaicin treatment on rat dorsal root ganglia and dorsal roots. J Physiol (Lond) 303:12P
Levi-Montalcini R (1982) Developmental neurobiology and the natural history of nerve growth factor. Ann Rev Neurosci 5:341–362
Levi-Montalcini R, Caramia F, Luse SA, Angeletti PU (1968) In vitro effects of the nerve growth factor on the fine structure of the sensory nerve cells. Brain Res 8:347–362
Mayer N, Lembeck F, Goedert M, Otten U (1982) Effects of antibodies against nerve growth factor on the post natal development of substance P containing neurons. Neurosci Lett 29:47–52
McLachlan EM, Jänig W (1983) The cell bodies of origin of sympathetic and sensory axons in some skin and muscle nerves of the cat hindlimb. J Comp Neurol 214:115–130
Mesulam MM (1978) Tetramethyl benzidine for horseradish peroxidase: a non-carcinogenic blue reaction-product with superior sensitivity for visualizing neural afferents and efferents. J Histochem Cytochem 26:106–117
Miller MS, Buck SH, Sipes IG, Yamamura HI, Burks TF (1982) Regulation of substance P by nerve growth factor: Disruption by capsaicin. Brain Res 250:193–196
Nagy JI (1982) Capsaicin: A chemical probe for sensory neuron mechanisms. In: Iversen, Iversen, Synder (eds) Handbook of psychopharmacology. Plenum Publishing Co. New York, 15:185–235
Nagy JI, Hunt SP, Iversen LL, Emson PC (1981) Biochemical and anatomical observations on the degeneration of peptidecontaining primary afferent neurons after neonatal capsaicin. Neuroscience 6:1923–1934
Otten U, Lorez HP, Businger F (1983) Nerve growth factor antagonizes the neurotoxic action of capsaicin on primary sensory neurones. Nature 301:515–517
Salt TE, Hill RG (1980) The effect of microiontophoretically applied capsaicin and substance P on single neurons in the rat and cat brain. Neurosci Lett 20:329–334
Scadding JW (1980) The permanent anatomical effects of neonatal capsaicin on somatosensory nerves. J Anat 131:473–484
Szolcsányi J (1982) Capsaicin type pungent agents producing pyrexiy. In: Milton AS (ed) Handbook of experimental pharmacology. Pyretics and antipyretics. Springer, New York, 60:437–478
Szolcsányi J, Jancsó A, Jóo F (1975) Functional and fine structural characteristics of the sensory neuron blocking effect of capsaicin. Naunyn Schmiedeberg's Arch Pharmacol 287:157–169
Taylor DCM, Korf H-W, Pierau Fr-K (1982) Distribution of sensory neurones of the pudendal nerve in the dorsal root ganglia and their projection to the spinal cord. Cell Tissue Res 226:555–564
Taylor DCM, Pierau Fr-K, Szolcsányi J (1984a) Long-lasting inhibition of horseradish peroxidase (HRP) transport in sensory nerves induced by capsaicin pretreatment of the receptive field. Brain Res 298:45–49
Taylor DCM, Pierau Fr-K, Szolcsányi J (1984b) Nerve growth factor prevents capsaicin induced inhibition of axoplasmic transport in small dorsal root ganglion neurones. Neurosci Lett Suppl 18:336
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Taylor, D.C.M., Pierau, F.K. & Szolcsányi, J. Capsaicin-induced inhibition of axoplasmic transport is prevented by nerve growth factor. Cell Tissue Res. 240, 569–573 (1985). https://doi.org/10.1007/BF00216346
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DOI: https://doi.org/10.1007/BF00216346