Summary
Specific secretory cells in the hypophyseal pars tuberalis of Djungarian hamsters maintained under different photoperiods were investigated immunocytochemically by means of the colloidal gold technique using antibodies against rat thyrotropin (TSH). Secretory cells of animals kept under long photoperiods (LD16:8) showed positive staining of secretory granules (diameters 90–130 nm), whereas other intracellular structures were free of immunoreactivity. In animals kept under short photoperiods (LD8:16) secretory cells displayed increased numbers of secretory granules, but these organelles were devoid of immunoreactivity. In contrast, immunoreactivity of thyrotropes in the pars distalis did not differ between the two groups of animals investigated. The present results confirm earlier light-microscopical studies that in the pars tuberalis specific secretory cells show TSH-like immunoreactivity; however, they differ in their reactivity pattern from classical thyrotropes in the pars distalis.
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Aguado LI, Hancke JL, Rodríguez S, Rodríguez EM (1982) Changes in the luteinizing hormone content of the rat pars tuberalis during estrous cycle and after lesions in the preoptic area. Neuroendocrinology 35:178–185
Asa SL, Kovacs K, Bilbao JM (1983) The pars tuberalis of the human pituitary. A histologie, immunohistochemical, ultrastructural and immunoelectron microscopic analysis. Virchows Arch [A] 399:49–59
Cameron E, Foster CL (1972) Some light and electron microscopical observations on the pars tuberalis of the pituitary gland of the rabbit. J Endocrinol 54:505–511
Dellmann H-D, Stoeckel ME, Hindelang-Gertner C, Porte A, Stutinski F (1974) A comparative ultrastructural study of the pars tuberalis of various mammals, the chicken and the newt. Cell Tissue Res 148:313–329
Fitzgerald KT (1979) The structure and function of the pars tuberalis of the vertebrate adenohypophysis. Gen Comp Endocrinol 37:383–399
Gross DS (1984) The mammalian hypophyseal pars tuberalis: A comparative immunocytochemical study. Gen Comp Endocrinol 56:283–298
Gross DS, Turgeon JL, Waring DW (1984) The ovine pars tuberalis: A naturally occurring source of partially purified gonadotropes which secrete luteinizing hormone in vitro. Endocrinology 114:2084–2091
Rütten A, Hewing M, Wittkowski W (1988) Seasonal ultrastructural changes of the hypophyseal pars tuberalis in the hedgehog (Erinaceus europaeus L.). Acta Anat 133:217–223
Stoeckel ME, Porte A (1984) Fine structure and development of the pars tuberalis in mammals. In: Motta PM (ed) Ultrastructure of endocrine cells and tissues. Martinus Nijhoff Publ, Boston pp 29–38
Williams LM, Morgan PJ (1988) Demonstration of melatoninbinding sites on the pars tuberalis of the rat. J Endocrinol 119:R1-R3
Wittkowski W, Hewing M, Hoffmann K, Bergmann M, Fechner J (1984) Influence of photo-period on the ultrastructure of the hypophyseal pars tuberalis of the Djungarian hamster, Phodopus sungorus. Cell Tissue Res 238:213–216
Wittkowski W, Bergmann M, Hoffmann K, Pera F (1988) Photoperiod-dependent changes in TSH-like immunoreactivity of cells in the hypophyseal pars tuberalis of the Djungarian hamster, Phodopus sungorus. Cell Tissue Res 251:183–187
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Bergmann, M., Wittkowski, W. & Hoffmann, K. Ultrastructural localization of thyrotropin (TSH)-like immunoreactivity in specific secretory cells of the hypophyseal pars tuberalis in the Djungarian hamster, Phodopus sungorus . Cell Tissue Res. 256, 649–652 (1989). https://doi.org/10.1007/BF00225616
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DOI: https://doi.org/10.1007/BF00225616