Summary
Isolated glomeruli of the rainbow trout have been exposed in vitro to125I-angiotensin II (0.88 × 10−9 M) and binding sites located by light-microscopic autoradiography. These studies provide evidence of specific binding of angiotensin II by glomeruli. Binding was significantly inhibited by excess (10−5 M) unlabelled angiotensin II, but a high degree of non-specific binding also occurred. The mammalian competitive antagonist, saralasin (3 × 10−7 M) did not influence125I-angiotensin II binding to fish glomeruli. Intense binding of125I-angiotensin II was noted at the vascular pole of some glomeruli.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bianchi C, Gutkowska J, Thibault G, Garcia R, Genest J, Cantin M (1986) Distinct localization of atrial natriuretic factor and angiotensin II binding sites in the glomerulus. Am J Physiol 251:F594–602
Brown JA (1985) Renal microvasculature of the rainbow trout,Salmo gairdneri: Scanning electron microscopy of corrosion casts of glomeruli. Anat Rec 213:505–513
Brown JA, Oliver JA (1985) The renin angiotensin system and single nephron glomerular structure and function in the trout,Salmo gairdneri. In: Lofts B, Holmes WN (eds) Current trends in comparative endocrinology. Hong Kong University Press, pp 905–909
Brown JA, Oliver JA, Henderson IW, Jackson BA (1980) Angiotensin and single nephron glomerular function in the troutSalmo gairdneri. Am J Physiol 239:R509–514
Brown JA, Taylor SM, Gray CJ (1983) Glomerular ultrastructure of the trout,Salmo gairdneri. Glomerular capillary epithelium and effects of environmental salinity. Cell Tissue Res 230:205–218
Caldicott WJH, Taub KJ, Margulies SS, Hollenberg NK (1981) Angiotensin receptors in glomeruli differ from those of renal arterioles. Kidney Int 19:687–693
Capponi AM, Aguilera G, Fakunding JL, Catt KJ (1981) Angiotensin II: Receptors and mechanisms of action. In: Soffer RF (ed) Biochemical regulation of blood pressure. John Wiley and Sons, New York, pp 205–262
Carroll RG (1981) Vascular response of the dogfish and sculpin to angiotensin II. Am J Physiol 240:R139–143
Catt KJ, Aguilera G (1980) Angiotensin II receptors. In: Schulster D, Ecvitski A (eds) Cellular receptors for hormones and neurotransmitters. John Wiley and Sons, New York, pp 233–251
Christensen JA, Knutsson S, Bohle A (1982) Granular epithelioid cells in the kidney of the Atlantic salmon (Salmo salar). Kidney Int 22 [Suppl 12]:S179–184
Churchill P, Malvin R, Churchill M, Beasley D, Shier D (1985) Antidiuretic effect of Sar1 Val5 Ala8 angiotensin II inLophius americanus. J Exp Zool 233:15–20
Gray CJ, Brown JA (1985) Renal and cardiovascular effects of angiotensin II in the rainbow trout,Salmo gairdneri. Gen Comp Endocrinol 59:375–381
Gray CJ, Brown JA (1987) Glomerular ultrastructure of the trout,Salmo gairdneri: Effects of angiotensin and adaptation to seawater. Cell Tissue Res 249:437–442
Khosla MC, Bumpus FM, Nishimura H, Opdyke DF, Coviello A (1983) Synthesis of nonmammalian angiotensins and their comparative pressor properties in dogfish shark, domestic chicken, and rat. Hypertension 5 [Suppl V]:V22–28
Khosla MC, Nishimura H, Hasegawa Y, Bumpus FM (1985) Identification and synthesis of (1-Asparagine, 5-Valine, P-Glycine Angiotensin I produced from plasma of American eelAnguilla rostrata. Gen Comp Endocrinol 57:223–233
Meddis K (1984) Statistics using ranks. Blackwell, Oxford
Nishimura H, Norton VM, Bumpus FM (1978) Lack of specific inhibition of angiotensin II in eels by angiotensin antagonists. Am J Physiol 235:H95–103
Osborne MJ, Droz B, Meyer P, Morel F (1975) Angiotensin II: Renal localization in glomerular mesangial cells by autoradiography. Kidney Int 8:245–254
Perrott MN (1988) The renin-angiotensin system and osmoregulation in fish. PhD thesis, University of Manchester, UK
Phillips HJ (1973) Dye exclusion test for cell viability. In: Kruse PF, Patterson MK (eds) Tissue culture — methods and applications. Academic Press, London, pp 406–408
Salancinski PRP, McLean C, Sykes JEC, Clement-Jones VV, Lowry PJ (1981) Iodination of proteins, glycoproteins, and peptides using solid-phase oxidizing agent, 1,3,4,6-tetrachloro-3, 6-diphenyl glycoluril (iodogen). Analyt Biochem 117:136–146
Sernia C (1984) Separation of radioiodinated angiotensins by chromatofocusing in minicolumns. Anal Biochem 138:303–308
Skorecki KL, Ballerman BJ, Rennke HG, Brenner BM (1983) Angiotensin II receptor regulation in isolated renal glomeruli. Fed Proc 42:3064–3070
Sraer J, Baud L, Cosyns J-P, Verroust P, Nivez M-P, Ardaillou R (1977) High affinity binding of125I-angiotensin II to rat glomerular basement membranes. J Clin Invest 59:69–81
Soffer RL, Kiron MAR, Rosenberg E, Bandyopadhyay S (1988) Angiotensin Receptor: Properties and relevance to the development of antagonists. Kidney Int 34 [Suppl 26]:S69–72
Taugner R, Hackenthal E (1981) Angiotensin II in epithelioid (renin containing) cell of rat kidney. Histochemistry 72:499–509
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Brown, J.A., Taylor, S.M. & Gray, C.J. Glomerular binding of angiotensin II in the rainbow trout,Salmo gairdneri . Cell Tissue Res. 259, 479–482 (1990). https://doi.org/10.1007/BF01740774
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01740774