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Cytokeratin 18 is an M-cell marker in porcine Peyer's patches

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Abstract

The intermediate filaments of the dome epithelium of porcine Peyer's patches were studied by immunohistochemistry. The labelling patterns of monospecific antibodies directed against cytokeratins 8, 18 and 19 differed considerably. About 40% of the dome epithelial cells were intensely labelled by three different anti-cytokeratin 18 antibodies, indicating that large amounts of cytokeratin 18 are present in these cells. In order to verify that these cytokeratin-18-immunoreactive cells were M-cells, uptake studies using fluorescein-labelled yeast particles were performed. Numerous yeast particles were found exclusively in dome epithelial cells that were highly positive for cytokeratin 18, thus representing M-cells. In contrast, the content of cytokeratin 19 in M-cells was lower than that in neighbouring enterocytes. The labelling intensity of cytokeratin 8 did not differ between M-cells and enterocytes. In addition, the absence of vimentin and desmin from the dome epithelium of porcine Peyer's patches was demonstrated. The results show (1) that porcine M-cells differ from enterocytes in the composition of their cytoskeleton, (2) that cytokeratin 18 is a useful marker for detecting porcine M-cells and (3) that this marker directly correlas with M-cell function.

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References

  • Adams JC, Watt FM (1988) An unusual strain of human keratinocytes which do not stratify or undergo terminal differentiation in culture. J Cell Biol 107:1927–1938

    Google Scholar 

  • Altmannsberger M, Osborn M, Schauer A, Weber K (1981) Antibodies to different intermediate filament proteins. Cell typespecific markers on paraffin-embedded human tissues. Lab Invest 45:427–434

    Google Scholar 

  • Amerongen HM, Weltzin R, Mack JA, Winner LS, Michetti P, Apter FM, Kraehenbuhl JP, Neutra MR (1992) M cell-mediated antigen transport and monoclonal IgA antibodies for mucosal immune protection. Ann NY Acad Sci 664:18–26

    Google Scholar 

  • Bartek J, Bartkova J, Taylor-Papadimitriou J, Rejthar A, Kovarik J, Lukas Z, Vojtesek B (1986) Differential expression of keratin 19 in normal human epithelial tissues revealed by monospecific monoclonal antibodies. Histochem J 18:565–575

    Google Scholar 

  • Binns RM, Licence ST (1985) Patterns of migration of labelled blood lymphocyte subpopulations: evidence for two types of Peyer's patch in the young pig. Adv Exp Med Biol 186:661–668

    Google Scholar 

  • Bye WA, Allan CH, Trier JS (1984) Structure, distribution, and origin of M cells in Peyer's patches of mouse ileum. Gastroenterology 86:789–801

    Google Scholar 

  • Chu RM, Glock RD, Ross RF (1979) Gut-associated lymphoid tissues of young swine with emphasis on dome epithelium of aggregated lymph nodules (Peyer's patches) of the small intestine. Am J Vet Res 40:1720–1728

    Google Scholar 

  • Demel M, Heyer G, Knochenhauer S, Schmidt MA (1992) Cytokeratin-8 as possible intracellular marker for M-cells of rat Peyer's patches (abstract). 7th International Congress of Mucosal Immunology, Prague, p 45

  • Franke WW, Schmid E, Osborn M, Weber K (1978) Different intermediate sized filaments distinguished by immunofluorescence microscopy. Proc Natl Acad Sci USA 75:5034–5038

    Google Scholar 

  • Franke WW, Appelhans B, Schmid E, Freudenstein C (1979) The organization of cytokeratin filaments in the intestinal epithelium. Eur J Cell Biol 19:255–268

    Google Scholar 

  • Franke WW, Winter S, Grund C, Schmid E, Schiller DL, Jarasch ED (1981) Isolation and characterization of desmosome-associated tonofilaments from rat intestinal brush border. J Cell Biol 90:116–127

    Google Scholar 

  • Gebert A, Hach G (1992) Vimentin antibodies stain membranous epithelial cells in the rabbit bronchus-associated lymphoid tissue (BALT). Histochemistry 98:271–273

    Google Scholar 

  • Gebert A, Hach G (1993) Differential binding of lectins to M-cells and enterocytes in the rabbit cecum. Gastroenterology 105:1350–1361

    Google Scholar 

  • Gebert A, Hach G, Bartels H (1992) Co-localization of vimentin and cytokeratins in M-cells of rabbit gut-associated lymphoid tissue (GALT). Cell Tissue Res 269:331–340

    Google Scholar 

  • Geiger B (1987) Intermediate filaments. Looking for a function. Nature 329:392–393

    Google Scholar 

  • Giaffer MH, Clark A, Holdsworth CD (1992) Antibodies to Saccharomyces cerevisiae in patients with Crohn's disease and their possible pathogenic importance. Gut 33:1071–1075

    Google Scholar 

  • Gigi-Leitner O, Geiger B, Levy R, Czernobilsky B (1986) Cytokeratin expression in squamous metaplasia of the human uterine cervix. Differentiation 31:191–205

    Google Scholar 

  • Gown AM, Vogel AM (1982) Monoclonal antibodies to intermediate filament proteins of human cells: unique and cross-reacting antibodies. J Cell Biol 95:414–424

    Google Scholar 

  • Ingber DE (1993) Cellular tensegrity: defining new rules of biological design that govern the cytoskeleton. J Cell Sci 104:613–627

    Google Scholar 

  • Inman LR, Cantey JR (1983) Specific adherence of Escherichia coli (Strain RDEC-1) to membranous (M) cells of the Peyer's patch in Escherichia coli diarrhea in the rabbit. J Clin Invest 71:1–8

    Google Scholar 

  • Jepson MA, Mason CM, Bennett MK, Simmons NL, Hirst BH (1992) Co-expression of vimentin and cytokeratins in M cells of rabbit intestinal lymphoid follicle-associated epithelium. Histochem J 24:33–39

    Google Scholar 

  • Jepson MA, Simmons NL, Savidge TC, James PS, Hirst BH (1993) Selective binding and transcytosis of latex microspheres by rabbit intestinal M cells. Cell Tissue Res 271:399–405

    Google Scholar 

  • Lazarides E (1980) Intermediate filaments as mechanical integrators of cellular space. Nature 283:249–256

    Google Scholar 

  • Levy R, Czernobilsky B, Geiger B (1988) Subtyping of epithelial cells of normal and metaplastic human uterine cervix, using polypeptide-specific cytokeratin antibodies. Differentiation 39:185–196

    Google Scholar 

  • Lunney JK (1993) Characterization of swine leukocyte differentiation antigens. Immunol Today 14:147–148

    Google Scholar 

  • Moll R, Franke WW, Schiller DL, Geiger B, Krepler R (1982) The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell 31:11–24

    Google Scholar 

  • Neutra MR, Kraehenbuhl J-P (1992) Transepithelial transport and mucosal defence I: the role of M cells. Trends Cell Biol 2:134–138

    Google Scholar 

  • Osborn M, Debus E, Weber K (1984) Monoclonal antibodies specific for vimentin. Eur J Cell Biol 34:137–143

    Google Scholar 

  • Owen RL (1977) Sequential uptake of horseradish peroxidase by lymphoid follicle epithelium of Peyer's patches in the normal unobstructed mouse intestine: an ultrastructural study. Gastroenterology 72:440–451

    Google Scholar 

  • Owen RL, Bhalla DK (1983) Cytochemical analysis of alkaline phosphatase and esterase activities and of lectin-binding and anionic sites in rat and mouse Peyer's patch M cells. Am J Anat 168:199–212

    Google Scholar 

  • Owen RL, Jones AL (1974) Epithelial cell specialization within human Peyer's patches: an ultrastructural study of intestinal lymphoid follicles. Gastroenterology 66:189–203

    Google Scholar 

  • Owen RL, Nemanic P (1978) Antigen processing structures of the mammalian tract: an SEM study of lymphoepithelial organs. Scanning Electron Microsc 11:367–378

    Google Scholar 

  • Owen RL, Pierce NF, Apple RT, Cray WC (1986) M cell transport of Vibrio cholerae from the intestinal lumen into Peyer's patches: a mechanism for antigen sampling and for microbial transepithelial migration. J Infect Dis 153:1108–1118

    Google Scholar 

  • Pabst R (1987) The anatomical basis for the immune function of the gut. Anat Embryol (Berl) 176:135–144

    Google Scholar 

  • Pappo J (1989) Generation and characterization of monoclonal antibodies recognizing follicle epithelial M cells in rabbit gut-associated lymphoid tissues. Cell Immunol 120:31–41

    Google Scholar 

  • Pappo J, Ermak TH, Steger HJ (1991) Monoclonal antibody-directed targeting of fluorescent polystyrene microspheres to Peyer's patch M cells. Immunology 73:277–280

    Google Scholar 

  • Sicinski P, Rowinski J, Warchol JB, Bem W (1986) Morphometric evidence against lymphocyte-induced differentiation of M cells from absorptive cells in mouse Peyer's patches. Gastroenterology 90:609–616

    Google Scholar 

  • Smith MW, Peacock MA (1980) “M” cell distribution in follicle-associated epithelium of mouse Peyer's patch. Am J Anat 159:167–175

    Google Scholar 

  • Sun TT, Shih C, Green H (1979) Keratin cytoskeletons in epithelial cells of internal organs. Proc Natl Acad Sci USA 76:2813–2817

    Google Scholar 

  • Tölle HG, Weber K, Osborn M (1985) Microinjection of monoclonal antibodies specific for one intermediate filament protein in cells containing multiple keratins allow insight into the composition of particular 10 nm filaments. Eur J Cell Biol 38:234–244

    Google Scholar 

  • Torres-Medina A (1981) Morphologic characteristics of the epithelial surface of aggregated lymphoid follicles (Peyer's patches) in the small intestine of newborn gnotobiotic calves and pigs. Am J Vet Res 42:232–236

    Google Scholar 

  • Walker RI, Schmauder-Chock EA, Parker JL (1988) Selective association and transport of Campylobacter jejuni through M cells of rabbit Peyer's patches. Can J Microbiol 34:1142–1147

    Google Scholar 

  • Weltzin R, Lucia-Jandris P, Michetti P, Fields BN, Kraehenbuhl JP, Neutra MR (1989) Binding and transepithelial transport of immunoglobulins by intestinal M cells: demonstration using monoclonal IgA antibodies against enteric viral proteins. J Cell Biol 108:1673–1685

    Google Scholar 

  • Wolf JL, Rubin DH, Finberg R, Kauffman RS, Sharpe AH, Trier JS, Fields BN (1981) Intestinal M-cells: a pathway for entry of reovirus into the host. Science 212:471–472

    Google Scholar 

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  1. Abteilung Anatomie 2, Medizinische Hochschule Hannover, D-30623, Hannover, Germany

    Andreas Gebert, Hermann-Josef Rothkötter & Reinhard Pabst

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  1. Andreas Gebert
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  2. Hermann-Josef Rothkötter
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  3. Reinhard Pabst
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Gebert, A., Rothkötter, HJ. & Pabst, R. Cytokeratin 18 is an M-cell marker in porcine Peyer's patches. Cell Tissue Res 276, 213–221 (1994). https://doi.org/10.1007/BF00306106

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  • DOI: https://doi.org/10.1007/BF00306106

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