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Growth and nutrient uptake of sorghum cultivated with vesicular-arbuscular mycorrhiza isolates at varying pH

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Abstract

This study was conducted to determine the effects of different pH regimes on root colonization with four vesicular-arbuscular mycorrhiza (VAM) isolates, and VAM effects on host plant growth and nutrient uptake. Sorghum [Sorghum bicolor (L.) Moench] was grown at pH 4.0, 5.0, 6.0 and 7.0 (±0.1) in hydroponic sand culture with the VAM isolates Glomus etunicatum UT316 (isolate E), G. intraradices UT143 (isolate I), G. intraradices UT126 (isolate B), and an unknown Glomus isolate with no INVAM number (isolate A). Colonization of roots with the different VAM isolates varied differentially with pH. As pH increased, root colonization increased with isolates B and E, remained unchanged with isolate I, and was low at pH 4.0 and high at pH 5.0, 6.0, and 7.0 with isolate A. Isolates E and I were more effective than isolates A and B in promoting plant growth irrespective of pH. Root colonization with VAM appeared to be independent of dry matter yields or dry matter yield responsiveness (dry matter produced by VAM compared to nonmycorrhizal plants). Dry matter yield responsiveness values were higher in plants whose roots were colonized with isolates E and I than with isolates A and B. Shoot P concentrations were lower in plants colonized with isolates E and I than with isolates A and B or nonmycorrhizal plants. This was probably due to the dilution effect of the higher dry matter yields. Neither the VAM isolate nor pH had an effect on shoot Ca, Mg, Zn, Cu, and Mn concentrations, while the VAM isolate affected not only P but also S, K, and Fe concentrations. The pH x VAM interaction was significant for shoot K, Mg, and Cu concentrations.

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References

  • Bartolome HT, Schenck NC (1990) Response of selected VA mycorrhizal fungi to soil acidity and aluminium toxicity. In: Innovation and hierarchical integration. Abstracts of the 8th North American Conference on Mycorrhizae, Jackson, Wyo, 5–8 Sept 1990. San Diego State University, San Diego, Calif University of Wyoming, Laramie, Wyo, p 18

    Google Scholar 

  • Clark RB (1982) Nutrient solution growth of sorghum and corn in mineral nutrition studies. J Plant Nutr 5:1039–1057

    Google Scholar 

  • Daniels BA, Trappe JM (1980) Factors affecting spore germination of the vesicular-arbuscular mycorrhizal fungus, Glomus epigaeus. Mycologia 72:457–471

    Google Scholar 

  • Davis EA, Young JL, Linderman RG (1983) Soil lime level (pH) and VA-mycorrhiza effects on growth responses of sweetgum seedlings. Soil Sci Soc Am J 47:251–256

    Google Scholar 

  • Dissing-Nielsen J (1989) The effect of VAM on growth and uptake of nutrients in lucerne. Agric Ecosyst Environ 29:99–102

    Google Scholar 

  • El-Kherbawy M, Angle JS, Heggo A, Chaney RL (1989) Soil pH, rhizobia, and vesicular-arbuscular mycorrhizae inoculation effects on growth and heavy metal uptake of alfalfa (Medicago sativa L.). Biol Fertil Soils 8:61–65

    Google Scholar 

  • Fabig B, Moawad AM, Achtnich W (1989) Effect of VA mycorrhiza on dry weight and phosphorus content in shoots of cereal crops fertilized with rock phosphates at different soil pH and temperature lelvels. Z Pflanzenernähr Bodenkd 152:255–259

    Google Scholar 

  • Foy CD, Chaney RL, White MC (1978) The physiology of metal toxicity in plants. Annu Rev Plant Physiol 29:511–566

    Google Scholar 

  • Giovannetti M, Mosse B (1980) An evalutation of techniques for measuring vesicular-arbuscular mycorrhizal infection in roots. New Phytol 84:489–500

    Google Scholar 

  • Graham JH, Leonard RT, Menge JA (1981) Membrane-mediated decrease in root exudation responsible for phosphorus inhibition of vesicular-arbuscular mycorrhiza formation. Plant Physiol 68:548–552

    CAS  Google Scholar 

  • Green NE, Graham SO (1976) The influence of pH on the germination of vesicular-arbuscular mycorrhizal spores. Mycologia 68:929–934

    Google Scholar 

  • Hayman DS, Tavares M (1985) Plant growth responses to vesicular-arbuscular mycorrhiza XV. Influence of soil pH on the symbiotic efficiency of different endophytes. New Phytol 100:367–377

    Google Scholar 

  • Killham K (1985) Vesicular-arbuscular mycorrhizal mediation of trace and minor element uptake in perennial grasses: relation to livestock herbage. In: Fitter AH, Atkinson D, Read DJ, Usher MB (eds) Ecological interactions in soil: plants, microbes and animals. Blackwell Scientific, London, pp 225–232

    Google Scholar 

  • Knudsen D, Clark RB, Denning JL, Pier PA (1981) Plant analysis of trace elements by X-ray. J Plant Nutr 3:61–75

    Google Scholar 

  • Koide R (1991) Nutrient supply, nutrient demand and plant response to mycorrhizal infection. New Phytol 117:365–386

    Google Scholar 

  • Koske RE, Gemma JN (1989) A modified procedure for staining roots to detect VA mycorrhizas. Mycol Res 4:496–505

    Google Scholar 

  • Koslowsky SD, Boerner REJ (1989) Interactive effects of aluminum, phosphorus and mycorrhizae on growth and nutrient uptake of Panicum virgatum L. (Poacea). Environ Pollut 61:107–125

    Google Scholar 

  • Kucey RMN, Janzen HH (1987) Effects of VAM and reduced nutrient availability on growth and phosphorus and micronutrient uptake of wheat and field beans under greenhouse conditions. Plant Soil 104:71–78

    Google Scholar 

  • Lucas RE, Knezek BD (1972) Climatic and soil conditions promoting micronutrient deficiencies in plants. In: Mortvedt JJ, Giordano PM, Lidsay WL (eds) Micronutrients in agriculture. Soil Science Society of America, Madison, Wis, pp 265–288

    Google Scholar 

  • Marschner H (1986) Mineral nutrition of higher plants. Academic Press, San Diego

    Google Scholar 

  • Menge JA, Jarrell WM, Labanauskas CK, Ojala JC, Huszar C, Johnson ELV, Sibert D (1982) Predicting mycorrhizal dependency of Troyer citrange on Glomus fasciculatus in Californian citrus soils and nursery mixes. Soil Sci Soc Am J 46:762–768

    Google Scholar 

  • Mosse B (1972a) Effects of different Endogone strains on the growth of Paspalum notatum. Nature 239:221–223

    Google Scholar 

  • Mosse B (1972b) The influence of soil type and Endogone strain on the growth of mycorrhizal plants in phosphate deficient soils. Rev Ecol Biol Sol 9:529–537

    Google Scholar 

  • Pacovsky RS (1986) Micronutrient uptake and distribution in mycorrhizal or phosphorus-fertilized soybeans. Plant Soil 95:379–388

    Google Scholar 

  • Phillips JM, Hayman DS (1970) Improved procedures for clearing roots and staining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection. Trans Br Mycol Soc 55:158–161

    Google Scholar 

  • Rai R (1988) Interaction response of Glomus albidus and Cicer rhizobium strains on iron uptake and symbiotic N2 fixation in calcareous soil. J Plant Nutr 11:863–869

    Google Scholar 

  • Raju PS, Clark RB, Ellis JR, Maranville JW (1988) Effects of VA mycorrhizae on growth and mineral uptake of sorghum grown at varied levels of soil acidity. Commun Soil Sci Plant Anal 19:919–931

    Google Scholar 

  • Raju PS, Clark RB, Ellis JR, Maranville JW (1990a) Effects of species of VA-mycorrhizal fungi on growth and mineral uptake of sorghum at different temperatures. Plant Soil 121:165–170

    Google Scholar 

  • Raju PS, Clark RB, Ellis JR, Maranville JW (1990b) Mineral uptake and growth of sorghum colonized with VA mycorrhiza at varied soil phosphorus levels. J Plant Nutr 13:843–859

    Google Scholar 

  • Ratnayake M, Leonard RT, Menge JA (1978) Root exudation in relation to supply of phosphorus and its possible relevance to mycorrhizal formation. New Phytol 81:543–552

    Google Scholar 

  • SAS User's Guide (1985) Statistics version, 5th edn. SAS Institute, Cary, NC

    Google Scholar 

  • Siqueira JO, Hubbell DH, Mahmud AW (1984) Effect of liming on spore germination, germ tube growth and root colonization by vesicular-arbuscular mycorrhizal fungi. Plant Soil 76:115–124

    Google Scholar 

  • Snellgrove RC, Stribley DP, Tinker PB, Lawlor DW (1986) The effect of vesicular-arbuscular mycorrhizal infection on photosynthesis and carbon distribution in leek plants. In: Gianinazzi-Pearson V, Gianinazzi S (eds) Physiological and genetical aspects of mycorrhizae. Proceedings of the 1st European Symposium on Mycorrhizae. Institut National de la Recherche Agronomique, Paris, pp 421–424

    Google Scholar 

  • Sparling GP, Tinker PB (1978) Mycorrhizal infection in pennine grassland I. Levels of infection in the field. J Appl Ecol 15:943–950

    Google Scholar 

  • Timmer LW, Leyden RF (1978) Stunting of citrus seedlings in fumigated soils in Texas and its correction by phosphorus fertilization and inoculation with mycorrhizal fungi. J Am Soc Hort Sci 103:533–537

    Google Scholar 

  • Wang GM, Stribley DP, Tinker PB, Walker C (1985) Soil pH and vesicular-arbuscular mycorrhizas. In: Fitter AH, Atkinson D, Read DJ, Usher MB (eds) Ecological interactions in soil: plants, microbes and animals. Blackwell Scientific, London, pp 219–224

    Google Scholar 

  • Yawney WJ, Schultz RC, Kormanik PP (1982) Soil phosphorus and pH influence the growth of mycorrhizal sweetgum. Soil Sci Soc Am J 46:1315–1320

    Google Scholar 

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Medeiros, C.A.B., Clark, R.B. & Ellis, J.R. Growth and nutrient uptake of sorghum cultivated with vesicular-arbuscular mycorrhiza isolates at varying pH. Mycorrhiza 4, 185–191 (1994). https://doi.org/10.1007/BF00206778

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