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Epstein-Barr virus infection in HIV-positive patients

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Abstract

The relationship between Epstein-Barr virus (EBV) viral load in peripheral blood and HIV infection was determined in 103 HIV-infected patients. Epstein-Barr virus was detected by polymerase chain reaction in 75 % of the patients, 21 % of whom had the more uncommon EBV subtype 2. The highest levels of EBV were found in patients with 100–400 CD4+ cells/mm3 and not in those with more profound immunosuppression. An association was identified between EBV load and HIV proviral levels (p<0.0001), an IgM response to EBV early antigens (p≤0.01) and p24 antigenemia (p<0.01 in patients with > 100 CD4+ cells), but not with other clinical or laboratory parameters. Combinations of different EBV and HIV parameters identified a subgroup of patients with a 2.2- to 4.8-fold risk of ≥ 35 % decline in CD4+ counts over six months. The association between EBV and HIV markers may reflect a significant pathogenic interaction between the two viruses.

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References

  1. MacMahon E, Glass J, Hayward S, Mann R, Becker P, Charade P, McArthur J, Ambinder R Epstein-Barr virus in AIDS-related primary central nervous system lymphoma. Lancet 1991, 338: 969–973.

    Article  PubMed  Google Scholar 

  2. Greenspan J, Greenspan D, Lennette E, Abrams D, Conant M, Petersen V, Freese U Replication of Epstein-Barr virus within the epithelial cells of oral hairy leukoplaquia, an AIDS-associated lesion. New England Journal of Medicine 1985, 313: 1564–1571.

    PubMed  Google Scholar 

  3. Koval V, Clrck C, Vaishnav M, Spector S, Spector D Human cytomegalovirus inhibits human immunodeficiency virus replication in cells productively infected by both viruses. Journal of Virology 1991, 65: 6969–6978.

    PubMed  Google Scholar 

  4. Laurence J, Astrin S Human immunodeficiency virus induction of malignant transformation in human B lymphocytes. Proceedings of the National Academy of Sciences of the USA 1991, 88: 7635–7639.

    PubMed  Google Scholar 

  5. Kenney S, Kamine J, Markovitz D, Fenrick R, Pagano J An Epstein-Barr virus immediate-early gene product trans-activates gene expression from the human immunodeficiency virus long terminal repeat. Proceedings of the National Academy of Sciences of the USA 1988, 85: 1652–1656.

    PubMed  Google Scholar 

  6. Pagano J, Kenney S, Markovitz D, Kamine J Epstein-Barr virus interactions with human retroviruses. Journal of Virological Methods 1988, 21: 229–239.

    Article  PubMed  Google Scholar 

  7. Rahman M, Kingsley L, Atchison R, Belle S, Breinig M, Ho M, Rinaldo C Reactivation of Epstein-Barr virus during early infection with human immunodeficiency virus. Journal of Clinical Microbiology 1991, 29: 1215–1220.

    PubMed  Google Scholar 

  8. Schattner A, Hanuka N, Sarov B, Sarov I, Handzel Z, Bentwich Z Sequential serological studies of homosexual men with and without HIV infection. Epstein-Barr virus activation preceding and following HN seroconversion. Clinical and Experimental Immunology 1991, 85: 209–213.

    PubMed  Google Scholar 

  9. Marèchal V, Nicolas J, Joah I, Costagliola D, Lefrère J Epstein-Barr virus transactivator antibodies in HIV infection. Lancet 1991, 338: 574.

    Article  Google Scholar 

  10. Birx D, Redfield R, Tosato G Defective regulation of Epstein-Barr virus infection in patients with acquired immunodeficiency syndrome or AIDS-related disorders. New England Journal of Medicine 1986, 314: 874–879.

    PubMed  Google Scholar 

  11. Sixbey J, Shirley P, Chesney P, Buntin D, Resnick L Detection of a second widespread strain of Epstein-Barr virus. Lancet 1989, ii: 761–765.

    Article  Google Scholar 

  12. Sculley T, Cross S, Borrow P, Cooper D Prevalence of antibodies to Epstein-Barr virus nuclear antigen 2B in persons infected with the human immunodeficiency virus. Journal of Infectious Diseases 1988, 158: 186–192.

    PubMed  Google Scholar 

  13. Kyaw M, Hurren L, Evans L, Moss D, Cooper D, Benson E, Esmore D, Sculley T Expression of B-type Epstein-Barr virus in HIV-infected patients and cardiac transplant recipients. AIDS Research and Human Retroviruses 1992, 8: 1869–1874.

    PubMed  Google Scholar 

  14. Swiss HIV Cohort Study Coordinating Center Documentation of the Swiss Cohort Study. Eidgenössische Drucksachen-Materialzentrale, Berne, 1991.

    Google Scholar 

  15. Kawsaki E Sample preparation from blood, cells, and other fluids. In: Innis D, Gelfand D, Sninsky J, White T (ed): PCR protocols: a guide to methods and applications. Academic Press, San Diego, 1990, p. 146–152.

    Google Scholar 

  16. Telenti A, Marshall W, Smith T Detection of Epstein-Barr virus by the polymerase chain reaction. Journal of Clinical Microbiology 1990, 28: 2187–2190.

    PubMed  Google Scholar 

  17. Telenti A Detection and typing of Epstein-Barr virus by the polymerase chain reaction. In: Persing D, Tenover F, White T, Smith T (ed): Diagnostic molecular microbiology. American Society for Microbiology Washington DC, 1993, p. 344–349.

    Google Scholar 

  18. Sample J, Young L, Martin B, Chatman T, Kieff E, Rickinson A, Kieff E Epstein-Barr virus types 1 and 2 differ in their EBNA-3A, EBNA-3B, and EBNA-3C genes. Journal of Virology 1990, 64: 4084–4092.

    PubMed  Google Scholar 

  19. Sixbey J, Shirley P, Sloas M, Raab-Traub N, Israele V A transformation-incompetent, nuclear antigen 2-deleted Epstein-Barr virus associated with replicative infection. Journal of Infectious Diseases 1991, 163: 1008–1015.

    PubMed  Google Scholar 

  20. Ou C, Kwok S, Mitchell S, Mack D, Sninsky J, Krebs J, Feorino P, Warfield D, Schochetman G DNA amplification for direct detection of HIV-1 in DNA of peripheral blood mononuclear cells. Science 1988, 239: 295–297.

    PubMed  Google Scholar 

  21. Telenti A, Imboden P, Germann D Competitive polymerase chain reaction using an internal standard: application to the quantitation of viral DNA. Journal of Virological Methods 1992, 39: 259–268.

    Article  PubMed  Google Scholar 

  22. Gorgievski-Hrisoho M, Hinderer W, Nebel-Schickel H, Horn J, Vornhagen R, Sonneborn H, Wolf H, Siegl G Serodiagnosis of infectious mononucleosis by using a recombinant Epstein-Barr virus antigens and enzyme-linked immunosorbent assay technology. Journal of Clinical Microbiology 1990, 28: 2305–2311.

    PubMed  Google Scholar 

  23. Saah A, Munoz A, Kuo V, Fox R, Kaslow R, Phair J, Rinaldo C, Detels R, Polk B Predictors of the risk of development of acquired immunodeficiency syndrome within 24 months among gay men seropositive for human immunodeficiency virus type 1: a report from the multicenter AIDS cohort study. American Journal of Epidemiology 1992, 135: 1147–1155.

    PubMed  Google Scholar 

  24. Morris J, Gardner M Calculating confidence intervals for relative risks, odds ratios, and standardised ratios and rates. In: Gardner M, Altman D (ed): Statistics with confidence. Universities Press, Belfast 1989, p. 50–63.

    Google Scholar 

  25. Magrath I Infectious mononucleosis and malignant neoplasia. In: Schlossberg D (ed): Infectious mononucleosis. Springer, New York 1989, p. 142–171.

    Google Scholar 

  26. Katz B, Raab-Traub N, Miller G Latent and replicative forms of Epstein-Barr virus DNA in lymphomas and lymphoproliferative diseases. Journal of Infectious Diseases 1989, 160: 589–598.

    PubMed  Google Scholar 

  27. Katz B, Saini U Presence of the diffuse early antigen of Epstein-Barr virus in lymphomas and lymphoproliferative disorders. American Journal of Pathology 1992, 140: 1247–1254.

    PubMed  Google Scholar 

  28. Chow KC, Nacilla N, Witzig T, Li CY Is persistent polyclonal B lymphocytosis caused by Epstein-Barr virus? A study with polymerase chain reaction and in situ hybridization. American Journal of Hematology 1992, 41: 270–275.

    PubMed  Google Scholar 

  29. Zon L, Groopman J Hematological manifestations of the human immunodeficiency virus. Seminars in Hematology 1988, 25: 208–218.

    PubMed  Google Scholar 

  30. Groopman J Management of the hematological complications of the human immunodeficiency virus infection. Reviews of Infectious Diseases 1990, 12: 931–937.

    PubMed  Google Scholar 

  31. Cohen J, Wang F, Mannick J, Kieff E Epstein-Barr virus nuclear protein 2 is a key determinant of lymphocyte transformation. Proceedings of the National Academy of Science of the USA 1989, 86: 9558–9562.

    Google Scholar 

  32. Yao Q, Rowe M, Young L, Rickinson A The Epstein-Barr virus carrier state: dominance of a single growth transforming isolate in the blood and in the oropharynx of healthy virus carriers. Journal of General Virology 1991, 72: 1579–1590.

    PubMed  Google Scholar 

  33. Rickinson A, Young L, Rowe M Influence of the Epstein-Barr virus nuclear antigen EBNA 2 on the growth phenotype of virus transformed B cells. Journal of Virology 1987, 61: 1310–1317.

    PubMed  Google Scholar 

  34. Boyle M, Sewell W, Sculley T.Apolloni A, Turner J, Swanson C, Penny R, Cooper D Subtypes of Epstein-Barr virus in human immunodeficiency virus-associated non-Hodgkin lymphoma. Blood 1991, 78: 3004–3011.

    PubMed  Google Scholar 

  35. Moss D, Misko I, Burrows S, Burman K, McCarthy R, Sculley T Cytotoxic T-cell clones discriminate between A- and B-type Epstein-Barr virus transformants. Nature 1988, 331: 719–721.

    Article  PubMed  Google Scholar 

  36. Israele V, Shirley P, Sixbey J Excretion of Epstein-Barr virus from the genital tract of men. Journal of Infectious Diseases 1991, 163: 1341–1343.

    PubMed  Google Scholar 

  37. Naher H, Gissmann L, Freese U, Petzoldt D, Helfrich S Subclinical Epstein-Barr virus infection of both the male and female genital tract. Indication for sexual transmission. Journal of Investigative Dermatology 1992, 98: 791–793.

    Article  PubMed  Google Scholar 

  38. Rowe M, Young L, Crocker J, Stokes H, Henderson S, Rickinson A Epstein-Barr virus-associated lymphoproliferative disease in the SCID mouse model: implications for the pathogenesis of EBV-positive lymphomas in man. Journal of Experimental Medicine 1991, 173: 147–158.

    Article  PubMed  Google Scholar 

  39. Centers for Disease Control Opportunistic non-Hodgkin's lyphomas among severely immunocompromised HIV-infected patients surviving for prolonged periods on antiretroviral therapy — United States. Morbidity and Mortality Weekly Report 1991, 40: 591–600.

    Google Scholar 

  40. Subar M, Neri A, Inghirami G, Knowles D, Dalla-Favera R Frequentc-myc oncogen activation and infrequent presence of Epstein-Barr virus genome in AIDS-associated lymphoma. Blood 1988, 72: 667–671.

    PubMed  Google Scholar 

  41. Ballerini P, Gaidano G, Gong J, Tassi V, Saglio G, Knowles D, Dalla-Favera R Molecular pathogenesis of HIV-associated lymphomas. AIDS Research and Human Retroviruses 1992, 8: 731–735.

    PubMed  Google Scholar 

  42. Niedobitek G, Herbst H, Young L, Brooks L, Masucci M, Crocker J, Rickinson A, Stein H Patterns of Epstein-Barr virus infection in non-neoplastic lymphoid tissue. Blood 1992, 79: 2520–2526.

    PubMed  Google Scholar 

  43. Yerly S, Chamot E, Hirschel B, Perrin L Quantitation of human immunodeficiency virus provirus and circulating virus: relationship with immunological parameters. Journal of Infectious Diseases 1992, 166: 269–276.

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Institute of Medical Microbiology, University of Berne, Friedbuehlstr. 51, CH-3010, Berne, Switzerland

    A. Telenti, F. Marchesi, D. Germann & L. Matter

  2. Medical Policlinic, University of Berne, Friedbuehlstr. 51, CH-3010, Berne, Switzerland

    D. E. Uehlinger & R. Malinverni

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  1. A. Telenti
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  2. D. E. Uehlinger
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Telenti, A., Uehlinger, D.E., Marchesi, F. et al. Epstein-Barr virus infection in HIV-positive patients. Eur. J. Clin. Microbiol. Infect. Dis. 12, 601–609 (1993). https://doi.org/10.1007/BF01973638

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