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Increased oxacillin activity associated with glycopeptides in coagulase-negative staphylococci

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Abstract

Vancomycin resistance in methicillin-resistant staphylococci presents a potential therapeutic problem. In order to understand the impact of low-level vancomycin resistance in coagulase-negative Staphylococci, stepwise selection of vancomycin resistance was accomplished by growingstaphylococcus haemolyticus in culture media with increasing concentrations of vancomycin. A >40-fold increase in susceptibility to β-lactam antibiotics was observed. No obvious alterations in the growth curve, the presence of themecA gene, total DNA restriction fragment length polymorphism (RFLP), β-lactamase production, or the crude protein fraction were detected in thestaphylococcus haemolyticus-derived clones when compared to the original isolate. The proportion of the oxacillin-heteroresistant population also remained similar. A comparable phenomenon occurred with the selection ofstaphylococcus epidermidis exhibiting low-level resistance to vancomycin. Additionally, it was observed that clinical isolates of coagulase-negative Staphylococci grown in the presence of sub-minimum inhibitory concentrations of either vancomycin or teicoplanin lost their high-level resistance to oxacillin. Checkerboard tests showed that the combination of vancomycin and oxacillin was synergistic for two isolates ofStaphylococcus haemolyticus, two of four isolates ofStaphylococcus epidermidis, and one isolate ofStaphylococcus hominis.

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Authors and Affiliations

  1. Department of Pathology and Laboratory Medicine, Albany Medical Center, New Scotland Avenue, A-22, 12208, Albany, New York, USA

    B. E. Domaracki, A. Evans, K. E. Preston & R. A. Venezia

  2. Graduate Hospital, 1800 Lombard Street, 19146, Philadelphia, Pennsylvania, USA

    H. Fraimow

Authors
  1. B. E. Domaracki
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  2. A. Evans
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  3. K. E. Preston
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  4. H. Fraimow
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  5. R. A. Venezia
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Domaracki, B.E., Evans, A., Preston, K.E. et al. Increased oxacillin activity associated with glycopeptides in coagulase-negative staphylococci. Eur. J. Clin. Microbiol. Infect. Dis. 17, 143–150 (1998). https://doi.org/10.1007/BF01691109

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