Electrochemical control of bacterial cell accumulation on submerged glass surfaces

Abstract

 Accumulation of bacterial cells on a transparent electrode was controlled by applying an alternating potential. Live and dead cells on a transparent electrode can be distinguished as blue and red cells, by staining with two fluorescent dyes, propidium iodide (PI) and 4′, 6-diamidino-   2-phenylindole dihydrochloride (DAPI) and propidium iodide (PI), respectively. Cells of the gram-negative marine bacterium, Vibrio alginolyticus attached to an indium/tin oxide (ITO) electrode were killed by applying a potential of 1.1 V versus saturated calomel electrode in seawater. By applying –0.4 V, 73% of the cells on the ITO electrode were desorbed in 10 min. Changes in pH and generation of chlorine were not observed after applying potentials in the range of –0.4∼1.2 V. After 21 h of immersion in V. alginolyticus cell suspension with the application of an alternating potential of 1.1 and –0.4 V, cells on the electrode were completely killed and the cell number decreased to 20% of that of no potential. Elution of indium and tin was not observed after 10 days application of alternating potential.