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Comparison of the histochemical and contractile properties of human triceps surae

  • Biomechanics
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Abstract

The purpose of the study was to compare the contractile properties determined from an electrically stimulated twitch with histochemically determined fibre type parameters of the human triceps surae. Muscle samples were obtained from the medial head of the gastrocnemius of ten male athletes. Ages ranged from 20 to 29 years. Muscle samples from the belly of the medial gastrocnemius muscle were obtained using the needle biopsy technique. The samples were treated histochemically for myosin ATPase to classify the fibres as either slow twitch (ST) or fast twitch (FT) and to determine fibre areas. Surface electrical stimulation was used to determine muscle twitch parameters. The contractile variables of the muscle twitch were latency (L), time to peak force (TPF), peak force (PF), half-contraction time (1/2 CT) and half-relaxation time (1/2 RT). Backward elimination procedures for dependent variables were used to determine which contractile properties best represented the histochemical profile of the muscles. Prediction formulas were developed for FT and ST percentages (R2=0·98, p<0·001), relative area percentage (R2=0·87, p<0·001), and ST area (R2=0·85, p<0·01). It was concluded that the use of the electrotensiometer (ETM) protocol was a valid testing procedure when studying physiological relationships of histochemical properties in intact human skeletal muscle.

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References

  • Belanger, A. Y. andMcComas, A. J. (1985) A comparison of contractile properties in human arm and leg muscles.Eur. J. Appl. Physiol.,54, 326–330.

    Google Scholar 

  • Belanger, A. Y. andMcComas, A. J. (1989) Contractile properties of human skeletal muscle in childhood and adolescence.,58, 563–567.

    Article  Google Scholar 

  • Benton, L. A., Baker, L. L., Bowan, B. B. andWalters, R. L. (1979)Functional electrical stimulation. Rehabilitation Engineering Center, Downey, California.

    Google Scholar 

  • Bergstrom, J. (1962) Muscle electrolytes in man.Scand. J. Clin. Lab. Invest.,68, Suppl., 11–13.

    Google Scholar 

  • Blomstrand, E. andEkblom, B. (1982) The needle biopsy technique for fiber determination in human skeletal muscle—a methodological study.Acta Physiol. Scand.,116, 437–442.

    Google Scholar 

  • Costill, D. L., Daniels, J., Evans, W., Fink, W., Krahenbuhl, G. andSaltin, B. (1976) Skeletal muscle enzymes and fiber composition in male and female track athletes.J. Appl. Physiol.,40, 149–154.

    Google Scholar 

  • Davies, C. T. M., Mecrow, I. K. andWhite, M. J. (1982) Contractile properties of the human triceps surae with some observations on the effects of temperature and exercise.Eur. J. Appl. Physiol.,49, 255–269.

    Article  Google Scholar 

  • Elder, G. C. B., Bradbury, K. andRoberts, R. (1982) Variability of fiber type distributions within human muscles.J. Appl. Physiol.,53, 1473–1480.

    Google Scholar 

  • Faulkner, J. A., Jones, D. A., Round, J. M. andEdwards, R. H. T. (1980) Dynamics of energetic processes in human muscle. InExercise bioenergetics and gas exchange.Cerretelli, P. andWhipp, B. J. (Eds.), Biomedical Press, Elsevier/North Holland, 81–90.

  • Foehring, R. C., Sypert, G. W. andMunson, J. B. (1988) Relation of whole muscle contractile properties to source of innervation.Exp. Neurol.,101, 366–373.

    Article  Google Scholar 

  • Froese, E. A. andHouston, M. E. (1985) Torque-velocity characteristics and muscle fibre type in human vastus lateralis.J. Appl. Physiol.,59, 309–314.

    Google Scholar 

  • Garnett, R. A. F., O'Donovan, M. J., Stephens, J. A. andTaylor, A. (1978) Motor unit organization of human medial gastrocnemius.J. Physiol.,287, 33–43.

    Google Scholar 

  • Gillespie, M. J., Gordon, T. andMurphy, P. R. (1986) Reinnervation of the lateral gastrocnemius and soleus muscles in the rat by their common nerve.,372, 485–500.

    Google Scholar 

  • Gravel, D., Belanger, A. Y. andRichards, C. L. (1987) Study of human muscle contraction using electrically evoked twitch responses during passive shortening and lengthening movements.Eur. J. Appl. Physiol.,56, 623–627.

    Google Scholar 

  • Henriksson-Larsen, K., Friden, J. andWretling, M. L. (1985) Distribution of fibre sizes in human skeletal muscle. An enzyme histochemical study in tibialis anterior.Acta Physiol. Scand.,123, 171–177.

    Article  Google Scholar 

  • Johnson, M. A., Polgar, J., Weightman, D. andAppleton, D. (1973) Data on the distribution of fibre types in thirty-six human muscles: an autopsy study.J. Neurol. Sci.,18, 111–129.

    Article  Google Scholar 

  • Mahon, M., Toman, A., William, P. L. T. andBagnall, K. M. (1984) Variability of histochemical and morphometric data from needle biopsy specimens of human quadriceps femoris muscle.,63, 85–100.

    Article  Google Scholar 

  • Marsh, E., Sale, D., McComas, A. J. andQuinlan, J. (1981) Influence of joint position on ankle dorsiflexion in humans.J. Appl. Physiol.,51, 160–167.

    Google Scholar 

  • McComas, A. S., Garner, S., Dantes, M., Hall, A. andQuartly, C. (1987) Three novel electrophysiologic tests for patients with muscle weakness.Birth Defects,23, 201–212.

    Google Scholar 

  • McNeal, D. R. andBaker, L. L. (1988) Effects of joint angle, electrodes and wave form on electrical stimulation of the quadriceps and hamstrings.Ann. Biomed. Eng.,16, 299–310.

    Article  Google Scholar 

  • Moulds, R. F. W., Young, A., Jones, D. A. andEdwards, R. H. T. (1977) A study of contractility, biochemistry and morphology of an isolated preparation of human skeletal muscle.Clin. Sci. Mol. Med.,52, 291–297.

    Google Scholar 

  • Petrella, R. J., Cunningham, D. A., Vandervoort, A. A. andPaterson, D. H. (1989) Comparison of twitch potentiation in the gastrocnemius of young and elderly men.Eur. J. Appl. Physiol.,58, 395–399.

    Article  Google Scholar 

  • Rice, C. L., Cunningham, D. H., Taylor, A. W. andPaterson, D. H. (1988) Comparison of the histochemical and contractile properties of human triceps surae.,58, 165–170.

    Article  Google Scholar 

  • Sale, D., Quinlan, J., Marsh, E., McComas, A. J. andBelanger, A. Y. (1982) Influence of joint position on ankle plantarflexion in humans.J. Appl. Physiol.,52, 1636–1642.

    Google Scholar 

  • Saltin, B., Henrikson, J., Nygaard, E. andAnderson, P. (1977) Fiber types and metabolic potentials of skeletal muscles in sedentary men and endurance runners.Ann. NY Acad. Sci.,302, 3–29.

    Google Scholar 

  • Saltin, B. andGollnick, P. D. (1983) Skeletal muscle adaptability. InHandbook of physiology.Peachy, L. D. (Ed.), American Physiological Society, Bethesda, 555–631.

    Google Scholar 

  • Tesch, P. andKarlsson, J. (1985) Muscle fibre types and sizes in trained and untrained muscles of elite athletes.J. Appl. Physiol.,59, 1716–1720.

    Google Scholar 

  • Thomas, D. O., Sagar, G., White, M. S. andDavies, C. T. M. (1988) Electrically evoked isometric and isokinetic properties of the triceps surae in young male subjects.Eur. J. Appl. Physiol.,58, 321–326.

    Article  Google Scholar 

  • Thomas, T. (1977) Fatty acid profile and skeletal muscle of trained and untrained men.J. Appl. Physiol.,43, 709–713.

    Google Scholar 

  • Thorstensson, A. (1976) Muscle strength, fiber types, and enzyme activities in man.Acta Physiol. Scand., Suppl. 443, 1–45.

    Google Scholar 

  • Vandervoort, A. A. andMcComas, A. J. (1983) A comparison of the contractile properties of the human gastrocnemius and soleus muscles.Eur. J. Appl. Physiol.,51, 435–440.

    Google Scholar 

  • Vandervoort, A. A. andMcComas, A. J. (1986) Contractile changes in opposing muscle of the human ankle joint with aging.J. Appl. Physiol.,61, 361–367.

    Google Scholar 

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  1. School of HPER, Bowling Green State University, 43403, Bowling Green, OH, USA

    C. L. Moss

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Moss, C.L. Comparison of the histochemical and contractile properties of human triceps surae. Med. Biol. Eng. Comput. 30, 600–604 (1992). https://doi.org/10.1007/BF02446791

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  • DOI: https://doi.org/10.1007/BF02446791

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