Abstract
Time-resolved tomography is performed in transillumination by using 527 nm picosecond pulses from a passively mode-locked doubled Nd/glass laser and a streak camera to select photons according to their flight time. This work reports on the increase in contrast of a time-resolved profile of a 2 mm radius opaque object embedded in a scattering medium, constituted of diluted milk in a 30 mm thick cell. For spatial analysis, the emerging photons are detected through a 6 mm slit at the outlet face of the cell. Transmission profiles obtained as a function of time show that the contrast is enhanced for the shortest flight times, while the ‘shadow’ of the object is no longer detected after about 100 ps. Moreover, improvements in contrast are studied for different configurations of the model, to analyse separately the role of collimated and scattered photons. It is expected that such a tomographic method based on time-resolved absorption could be applied to imaging for more complex biological structures in the red and near-infra-red range.
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Araki, R. andNashimoto, I. (1991) Near-infrared imaging in vivo: imaging of Hb oxygenation in living tissues. Proc. Time-Resolved Spectrosc. & Imag. of Tissues, SPIE,1431, 321–332.
Anderson-Engels, S., Berg, R., Svanberg, S. andJarlman, O. (1990) Time-resolved transillumination for medical diagnostics.Opt. Lett.,15, 1179–1181.
Arridge, S., van der Zee, P., Cope, M. andDelpy, D. (1990) New results for the development of infra-red absorption imaging. Proc. Biomed. Image Proc. Conf., Santa Clara, 12th–13th Feb. 1990, paper 1245-07.
Bartrum, R. J. andCrow, H. C. (1984) Transillumination light-scanning to diagnose breast cancer.Am. J. Radiol.,142, 409–414.
Chance, B., Leigh, J. S., Miyake, H., Smith, D. S., Nioka, S., Greenfeld, R., Finander, R., Kaufmann, K. andLevy, W. (1988) Comparison of time-resolved and-unresolved measurements of deoxyhemoglobin in brain.Proc. Nat. Acad. Sci. USA,85, 4971–4975.
Delpy, D. T., Cope, M., van der Zee, P., Arridge, S., Wray, S. andWyatt, J. (1988) Estimation of optical pathlength through tissue from direct time of flight measurement.Phys. in Med. & Biol.,33, 1433–1442.
Dilworth, D. S., Leith, E. N. andLopez, J. L. (1990) Imaging absorbing structures within thick diffusing media.Appl. Optics,29, 691–698.
Duguay, M. A. andMattick, A. T. (1971) Ultra high speed photography of picosecond light pulses and echo. —Ibid.,,10, 2162–2170.
Ghesquiere, S., Debray, S., Maarek, J. M., Fraysse, F., Besson, B., Bui-Mong-Hung andJarry, G. (1984) L'image par transillumination collimatée de tissus et d'organes de mammifères.Innov. Tech. Biol. Med.,5, 22–32.
Hebden, J. C. andKruger, R. A. (1990) Transillumination imaging performance: spatial resolution simulation studies.Med. Phys.,17, 41–47.
Hebden, J. C., Kruger, R. A. andWong, K. S. (1991) Time resolved imaging through a highly scattering medium.Appl. Optics,30, 788–794.
Jackson, P. C., Stevens, P. H., Smith, J. H., Kear, D., Key, H. andWells, P. N. (1987) The development of a system for transillumination computed tomography.Br. J. Radiol.,60, 375–380.
Jarry, G., Ghesquiere, S., Maarek, J. M., Fraysse, F., Debray, S., Bui-Mong-Hung andLaurent, D. (1984) Imaging mammalian tissues and organs using laser collimated transillumination.J. Biomed. Eng.,6, 70–73.
Jarry, G., Debray, S., Perez, J., Lebebvre, J. P., de Ficquelmont, M. andGaston, A. (1989)In vivo transillumination of the hand using near infrared laser pulses and differential spectroscopy, —Ibid.,,11, 293–299.
Jobsis, F. F. (1977) Non-invasive infrared monitoring of cerebral and myocardial oxygen insufficiency and circulation parameters.Science,198, 1264–1267.
Key, H., Jackson, P. C. andWells, P. N. T. (1988) New approaches to transillumination imaging.J. Biomed. Eng.,10, 113–118.
Lafreniere, R., Ashkar, F. S. andKetcham, A. S. (1986) Infrared light scanning of the breast.Am. Surg.,52, 123–128.
Lefebvre, J. P., Jarry, G. andJorand, C. (1989) Optical processing of images obtained by transillumination of heterogeneous scattering and absorbing media. Proc. Opt. Patt. Recog. II, SPIE,1134, 154–159.
Maarek, J. M., Jarry, G., DeCosnac, B., Lansiart, A. andBui-Mong-Hung (1984) A simulation method for the study of laser transillumination of biological tissues.Ann. Biomed.,12, 281–304.
Maarek, J. M., Jarry, G., Crowe, J. A., Bui-Mong-Hung andLaurent, D. (1986a) Simulation of laser tomoscopy in a heterogeneous biological medium.Med. & Biol. Eng. & Comput.,24, 407–414.
Maarek, J. M., Jarry, G., Debray, S., Deugnier, M. A., Besson, B. andBui-Mong-Hung (1986b) Simulation du comportement de la lumière dans les tissus biologiques.Innov. Tech. Biol. Med.,7, 293–308.
Martin, J. L., Lecarpentier, Y., Antonetti, A. andGrillon, G. (1980) Picosecond laser stereometry light scattering measurements on biological material.Med. & Biol. Eng. & Comput.,18, 250–252.
Oda, I., Ito, Y., Eda, H., Tamura, T., Takada, M., Abumi, R., Nagai, K., Nakagawa, H. andTamura, M. (1981) Noninvasive hemoglobin oxygenation monitor and computed tomography by NIR spectrophotometry. Proc. Time-Resolved Spectrosc. & Imag. of Tissues, SPIE,1431, 284–293.
Patterson, M. S., Chance, B. andWilson, B. C. (1989) Time resolved reflectance and transmittance for the non-invasive measurement of tissue optical properties.Appl. Optics,28, 2331–2336.
Smith, K. C. (1980) Common misconceptions about light. InLasers in photomedicine and photobiology, vol. 22.Pratesi, K. andSacchi, C. A. (Eds.), Springer-Verlag, 23–25.
Spears, K. G., Serafin, J., Abramson, N. H., Zhu, X. andBjelkhagen, H. (1989) Chrono-coherent imaging for medicine.IEEE Trans.,BME-36, 1210–1221.
Takigushi, Y., Aoshima, S. Tsuchiya, Y. andHiruma, T. (1986) Laser pulse tomography using a streak camera. Proc. Image Detection & Quality, SPIE,702, 147–150.
Toida, M., Kondo, M. andInaba, H. (1989) Optical heterodyne technique for achieving excellent image detection in highly scattering media such as biological substances and tissues. OSA Annual Meeting 1989, Tech. Digest Series, Opt. Soc. Am., Washington DC, Vol. 18, 233.
Watmough, D. J. (1982) Diaphanography-mechanism responsible for the images.Acta Radiol. Oncol.,21, 11–15.
Yamada, Y. andHasekawa, Y. (1991) Stimulation of time-resolved optical CT imaging. Proc. Time-Resolved Spectrosc. & Imag. of Tissues, SPIE,1431, 73–82.
Yamashita, Y., Suzuki, S., Miyaki, S. andHayakawa, T. (1988) The neonate brain (NIR) and breast imaging using transillumination.Photon Migration in Tissues,6.
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Jarry, G., Lefebvre, J.P., Debray, S. et al. Laser tomography of heterogeneous scattering media using spatial and temporal resolution. Med. Biol. Eng. Comput. 31, 157–164 (1993). https://doi.org/10.1007/BF02446674
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DOI: https://doi.org/10.1007/BF02446674