Summary
Microheterogeneity of alpha-1-acid glycoprotein was studied using affinity immunoelectrophoresis with concanavalin A as a ligand in the samples of serum and synovial fluid obtained at the same time from 22 patients with rheumatoid arthritis. Individuals with intercurrent infection or other illnesses were excluded from the study. The results were expressed as reactivity coefficient (RC). Disease activity was evaluated by Mallya-Mace Activity Score, Lansbury Joint Index and laboratory tests. In most of the studied samples the glycosylation pattern was similar, composed of a nonreactive variant and 2 reactive (the first and the second) with Con A variants. In seven samples of synovial fluid an extra third peak representative of the strongly reactive one with Con A fraction was observed. It was the cause of the remarkable elevation of AGP-RC. Moreover, the level of IgM and IgA RF was higher in the synovial fluid derived from these patients — with the presence of the third peak in AFF-EP with Con A — than in those without the considered fraction.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Arnet, F.C., Edworthy, S.M., Bloch, D.A., McShane, D.J., Fries, J.F., Cooper, N.S., Healey, L.A., Kaplan, S.R., Liang, M.H., Luthra, H.S., Medsper, T.A., Mithchel, D.M., Neustadt, D.H., Pinals, R.S., Schaller, J.G., Sharp, J.T., Wilder, R.L., Hunder, G.G. The American Rheumatism Association 1987 revised criteria for the classification of rheumatoid arthritis. Arthritis Rheum 1988, 31, 315–324.
Bog-Hansen, T.C. Crossed immuno-affinoelectrophoresis. Anal Biochem, 1973, 56, 480.
Breborowicz, J., Mackiewicz, A. Affinity electrophoresis for diagnosis of cancer and inflammatory conditions. Electrophoresis 1989, 10, 568–573.
Brozik, M., Rosztoczy, I., Meretey, K., Balint, G., Gaal, M., Balogh, Z., Bart, M., Mituszova, M., Velics, V., Falus, A. Interleukin 6 level in synovial fluid of patients with different arthritides: correlation with local IgM rheumatoid factor and systemic acute phase protein production. J Rheumatol 1992, 19, 63–68.
Fassbender, K., Zimmerli, W., Kissling, R., Sobieska, M., Aeschlimann, A., Kellner, M., Muller, W. Glycosylation of alpha 1-acid glycoprotein in relation to duration of disease in acute and chronic infection and inflammation. Clin Chim Acta 1991, 203, 315–327.
Karrer, U., Aeschlimann, A., Fassbender, K., Vogt, P., Muller, W. Interleukin-6 (IL-6), loslicher Interleukin-2-Rezeptor (sIL-2R) und Mikroheterogenitat des alpha-1 sauren Glykoproteins (AGP): neue Marker der Akut-Phase-Reaktion? Schweiz Med Wochenschr 1992, 122, 233–236.
Lansbury, J., Haut, D.D. Quantitation of the manifestations of rheumatoid arthritis. Area of joint surfaces as an index to total joint inflammation and deformity. Am J Med Sci 1956, 232, 150–155.
Laurell, C.B. Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies. Scand J Clin Lab Invest 1972, 124 (suppl.), 21–23.
Mackiewicz, A., Marcinkowska-Pieta, R., Ballou, S., Mackiewicz, S., Kushner, I. Microheterogeneity of alpha-1- acid glycoprotein in the detection of intercurrent infection in systemic lupus erythematosus. Arthritis Rheum 1987, 30, 5, 513–517.
Mackiewicz, A., Pawlowski, T., Mackiewicz-Pawlowska, A., Wiktorowicz, K., Mackiewicz, S. Microheterogeneity of alpha-1-acid glycoprotein as indicator of rheumatoid arthritis activity. Clin Chim Acta 1987, 163, 185–190.
Mackiewicz, A., Khan, M.A., Reynolds, T.L., Van der Linden, S., Kushner, I. Serum IgA, acute phase proteins and glycosylation of alpha-1-acid glycoprotein in ankylosing spondylitis. Ann Rheum Dis 1989, 48, 99–103.
Mackiewicz, A., Pawlowski, T. Microheterogeneity of alpha-1-acid glycoprotein in rheumatic diseases. In: Genetics, Biochemistry, Physiological Functions and Pharmacology of Alpha-1-acid Glycoprotein. Eds.: Bauman P. et al. New York, 1989, 219–222.
Mallya, R.K., Mace, B.E.W. The assessment of disease activity in rheumatoid arthritis using a multivariate analysis. Rheumatol Rehabil 1981, 20, 14–17.
Moynier, M., Abderrazik, M., Didry, C., Sany, J., Brochier, J. The B-cell repertoire in rheumatoid arthritis. III. Preferential homing of rheumatoid factor-producing B-cells precursors in the synovial fluid. Arthritis Rheum 1992, 35, 49–54.
Pawlowski, T., Aeschlimann, A., Kahn, M.F., Vaith, P., Mackiewicz, S.H., Muller, W. Microheterogeneity of acute phase proteins in the differentation of polymyalgia rheumatica from polymyositis. J Rheumatol 1990, 17, 1187–1192.
Pawlowski, T., Mackiewicz, A. Minor microheterogeneity of alpha-1-acid glycoprotein in rheumatoid arthritis. Prog Clin Biol Res, 1989, 300, 223–226.
Pawlowski, T., Mackiewicz, S.H., Mackiewicz, A. Microheterogeneity of alpha-1-acid glycoprotein in the detection of intercurrent infection in patients with rheumatoid arthritis. Arthritis Rheum 1989, 32, 347–351.
Rowley, M.J., Williamson, D.J., Mackay, I.R. Evidence for local synthesis of antibodies to denatured collagen in the synovium in rheumatoid arthritis. Arthritis Rheum 1987, 30, 1420–1425.
Sawada, T., Hirohata, S., Inoue, T., Ito, K. Correlation between rheumatoid factor and IL-6 activity in synovial fluids from patients with rheumatoid arthritis. Clin Exp Rheumatol 1991, 9, 363–368.
Stefanini, G.F., Mazzetti, M., Piccinini, G.C., Capelli, S., Baraldini, M., Gasbarini, G. Lymphocyte membrane alpha-1-acid glycoprotein: cellular synthesis during lymphocyte activation. Biochem Int 1989, 19, 397–403.
Steinbrocker, O., Traeger, C.M., Batterman, R.C. Therapeutic criteria for rheumtoid arthritis. JAMA 1949, 140, 650–662.
Tarkowski, A., Klareskog, L., Carlsten, H., Herberts, P., Koopman, W.J. Secretion of antibodies to types I and II collagen by synovial tissue cells in patients with rheumatoid arthritis. Arthritis Rheum 1989, 32, 1087–1092.
Warnatz, H., Ruschen S., Lemm, G. Interleukin 1 in the pathogenesis of chronic polyarthritis. Med Klin 1990, 85, 302–307.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Lacki, J.K., Klama, K., Samborski, W. et al. Comparison of microheterogeneity of alpha-1-acid-glycoprotein in serum and synovial fluid from rheumatoid arthritis patients. Clin Rheumatol 13, 598–604 (1994). https://doi.org/10.1007/BF02243001
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02243001