Abstract
Different forms of experimental allergic encephalomyelitis were obtained in 4 groups of guinea pigs: 7 adult Hartley guinea pigs (Group I), 12 adults of the same strain (Group II), 6 juvenile strain 2 guinea pigs (Group III) and 6 juvenile strain 13 animals (Group IV), by the injection of emulsions. Groups I and II received emulsions containing 250 mg and 500 mg respectively of fresh isologous spinal cord tissue, complete Freund adjuvant (CFA) and saline solution while Groups III and IV received an emulsion containing 120 mg of isologous spinal cord, CFA, saline solution and 15 mg of Mycobacterium tuberculosis. The increased antigen load induced a disease with delayed onset and prolonged progressive course (C-P-EAE) in Groups I and II, although 8 animals showed no symptoms of illness. The findings in C-P-EAE were large demyelinated plaques, perivenous fibrosis and large areas of infiltration. Demyelinated areas occurred within the spinal cord white matter only in two asymptomatic animals. C-P-EAE was obtained in 4 of the Strain 2 animals. In conclusion, the increased antigen load induced a range of lesions in Hartley guinea pigs, although not all animals were affected. C-P-EAE was induced also in strains of guinea pig other than the Heartley strain. These different reactions may have been the outcome of partial or complete inactivation of the cell-mediated response to the inoculated antigens.
Sommario
Differenti forme di encefalomielite allergica sperimentale sono state realizzate in quattro gruppi di cavie con iniezione di emulsioni: il primo gruppo era di 7 cavie di tipo Hartley, il secondo era formato da 12 adulti dello stesso ceppo, il terzo da 6 soggetti di ceppo giovanile 2 e il quarto da 6 animali del ceppo giovanile 13. I gruppi 1 e 2 ricevettero emulsioni contenenti rispettivamente 250 e 500 mg. di tessuto midollare isolago fresco con composto di Freund e soluzione salina mentre i grouppi 3 e 4 ricevettero un'emulsione con 120 mg. di midollo e gli altri componenti e in più 15 mg. di mycobacterium tubercolosis. Nel gruppo 1 e 2 si sviluppò una forma morbosa con ritardato avvio e un decorso prolungato e progressivo, benché 8 animali non abbiano presentato sintomi di malattia: si riscontrava nelle cavie malate la presenza di placche vaste di demielinizzazione con fibrosi perivenosa ed area estesa di infiltrazione. In 2 animali asintomatici si è constatata un'area di demielinizzazione nella sostanza bianca del midollo. La stessa forma a lento avvio e a decorso prolungato e progressivo si è verificata in 4 animali del ceppo 2. Risultò cosi che l'aumento degli antigeni ha prodotto importanti lesioni negli animali tipo Hartley anche se non tutti gli animali presentarono sintomi di malattia e lo stesso tipo di malattia è stato indotto anche in ceppi di cavie di tipo non Hartley. Queste reattività differenziate possono essere il risultato di una parziale o completa inattivazione della risposta mediata delle cellule agli antigeni inoculati.
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References
Ben Num A., Cohen I.R.:Spontaneous remission and acquired resistance to autoimmune encephalomyelitis (EAE) are associated with suppression of T cells reactivity: suppressed EAE effector T cells recovered as T cells lines. J. Immunol. 138: 1450–1457, 1982.
Cambi F., Lees M.B., Williams R.M., Macklin W.B.:Chronic experimental allergic encephalomyelitis produced by bovine proteolipid apoprotein. Immunologica studies in rabbits. Ann. Neurol. 13: 303–308, 1983.
Gambi D., Di Cesare N., Di Trapani G.:Modelli sperimentali delle sindromi demielinizzanti: l'encefalite sperim entale allergica. Atti del VII Corso Aggiornamento SIN Milano 19–20 Ottobre. Neurologia, Psichiatria, Scienze Umane. Pensiero Scientifico ed. 1984.
Hashim G.A.:Successfull immunization against experimental allergic encephalomyelitis with myelin basic proteir-sensitized allogenic lymphocites. Neurochem. Res. 6: 699–718, 1981.
Humphrey J.H.:Some reflections on principles and mechanisms of immunological tolerance. Br. Med. Bull. 32: 182–184, 1976.
Kies M.W., Alvord C.E.:Allergic Encephalomyelitis. Springfield, III, Thomas, 1959.
Lampert P.W.:Electron microscopic studies on ordinary and hyperacute experimental allergic encephalomyelitis. Acta Neuropathol. 9: 99–126, 1967.
Lassmann H., Wisniewski H.M.:Chronic Relapsing EAE. Time course of neurological Symptoms and Pathology. Acta Neuropathol., 43: 35–42, 1978.
Lassmann H., Wisniewski H.M.:Chronic Relapsing experimental allergic encephalomyelitis. Clinicopathological comparison with Multiple Sclerosis. Arch. Neurol., 436: 490–497, 1979.
Lassmann H.:Comparative neuropathology of Chronic Experimental allergic encephalomyelitis and Multiple Sclerosis. Springer Verlag; Berlin-Heidelberg New York, Tokyo, pp 135, 1983.
Levine S.:Hyperacute, Neutrophilic and Localized forms of experimental allergic encephalomyelitis: a review. Acta Neuropathol. (Berl) 28: 179–198, 1974.
Moore W., Traugott U., Stone S.H., Raine C.S.:Dose dependency of MBP-induced demyelination in the guinea pig. J. Neurol. Sci, 70, 197–205 1985.
Prineas J., Raine C.S., Wisniewski H.:An ultrastructural study of experimental demyelination and remyelination. III. Chronic experimental allergic encepahalomyelitis in the central nervous system. Lab. Inv. 21: 472–483.
Raine C.S.:Experimental allergic encephalomyelitis and related condition. In H.M. Zimmermann (ed), 1976.
Snyder D.M., Valsamis M.S., Stone S.M., Raine C.S.:Progressive and reparatory events in chronic experimental allergic encephalomyelitis. J. Neuropathol. Exp. Neurol. 34: 209–221, 1975.
Stone S.H., Lerner E.M.:Chronic disseminated allergic encephalomyelitis in guinea pigs. Ann. NY Acad. Sci., 122: 227–241, 1965.
Traugott U., Raine C.S.:Experimental allergic encephalomyelitis. Early T cells and TG cells are separate subpopulation. J. Neurol. Sci., 56: 17–22, 1983.
Traugott U.:Acute experimental autoimmune encephalomyelitis. Differences between T cells subsets in the blood and meningeal infiltrates in susceptible and resistant strains of guinea pigs. J. Neurol. Sci., 61: 81–91, 1983.
Yoshimura T., Kunhishita R., Sakai K., Endoh M., Namikawa T., Tabira T.:Chronic experimental allergic encephalomyelitis in guinea pigs induced by proteolipid protein. J. Neurol. Sci., 69, 47–58, 1985.
Williams R.M., Lees M.B., Cambi F., Macklin W.B.:Chronic experimental allergic encephalomyelitis induced in rabbits with bovine white matter proteolipid apoprotein. J. Neuropath. Exp. Neurol., 41: 508–521, 1982.
Wisniewski H.M., Keith A.B.:Chronic relapsing experimental encephalomyelitis. An experimental model of Multiple Sclerosis. Ann. Neurol., 1: 144–148, 1977.
Wisniewski H.M., Madrid R.E.:Chronic Progressive Experimental Allergic Encephalomyelitis (EAE) in adult guinea pigs. J. Neuropath. Exp. Neurol., 42: 243–255, 1983.
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Gambi, D., Di Cesare, N., Di Trapani, G. et al. Experimental allergic encephalomyelitis in guinea pig: variability of response to intradermal emulsion injection. Ital J Neuro Sci 10, 33–41 (1989). https://doi.org/10.1007/BF02333870
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DOI: https://doi.org/10.1007/BF02333870